Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,027,191	A→C	9.8%	H252Q (CAT→CAG)	vraS ←	Sensor protein VraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,027,191	0	A	C	9.8%	173.5 / 6.0	69	H252Q (CAT→CAG)	vraS	Sensor protein VraS
Reads supporting (aligned to +/- strand):  ref base A (33/28);  new base C (4/3);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCTTA  >  USA300TCH1516_ALE/2027051‑2027327                                                                                                                                             |                                                                                                                                           tcatcaACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACaaa                                                                                                                                            <  2:591102/141‑1 (MQ=255)  catcaACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAAt                                                                                                                                           >  1:496877/1‑141 (MQ=255)     aaaCATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGAt                                                                                                                                        <  2:194468/140‑1 (MQ=255)      aaCATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATc                                                                                                                                       <  1:254614/141‑1 (MQ=255)        cATTAAAACCTTTACCATTATGTTGAATTCTCAACAATAAATACTCGTCTTTATTAAACAATTCTACTGTCACCTGTGTACTGTTTGTATGTCGCAATGTATTCGTAATTGCTTCCTGTTTGATTCTTAGCAAATGATCtt                                                                                                                                     >  1:478929/1‑141 (MQ=255)        cATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCtt                                                                                                                                     <  1:151534/141‑1 (MQ=255)            aaaaCCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGGACAAATGTTCTTCAAt                                                                                                                                 >  2:83699/1‑141 (MQ=255)            aaaaCCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAAt                                                                                                                                 >  2:369268/1‑141 (MQ=255)                ccTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCt                                                                                                                             >  2:374284/1‑141 (MQ=255)                   ttACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTa                                                                                                                          >  1:435840/1‑141 (MQ=255)                        aTTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCAc                                                                                                                     <  1:497375/141‑1 (MQ=255)                          tATCCTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACtt                                                                                                                   <  1:139588/141‑1 (MQ=255)                           aTCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACttt                                                                                                                  <  1:94935/141‑1 (MQ=255)                               tGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCACTTTaaaa                                                                                                              >  1:373514/1‑141 (MQ=255)                                    tctcAACAATAAATAATCGTCTTGATTAAACAATTCTACTGTCACTTGTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTGAGGCACTTTAAAATCTTg                                                                                                         <  2:340055/141‑1 (MQ=255)                                     ctcAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTGAATACCTTTAGGCACTTTAAAATCTTGt                                                                                                        >  1:119706/1‑141 (MQ=255)                                     ctcAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGt                                                                                                        <  2:424702/141‑1 (MQ=255)                                       caacaaTAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTAt                                                                                                      <  2:372231/141‑1 (MQ=255)                                            ataaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATGTTGTTTTTcct                                                                                                 >  2:229347/1‑139 (MQ=255)                                             taaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTAACGTTTGAATGAAGCAATGTATTAGAAATTGATACCTGCGTATTTCTGAACAAATGAACATCAATACCTCTATGCACGTTAAAATCTTGTATTTCATg                                                                                                >  2:52620/1‑141 (MQ=255)                                              aaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTACATTCTTGTATTTCATGc                                                                                               <  2:461878/141‑1 (MQ=255)                                              aaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGc                                                                                               >  2:451930/1‑141 (MQ=255)                                              aaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGc                                                                                               <  2:48122/141‑1 (MQ=255)                                               aataatCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGca                                                                                              >  1:409008/1‑141 (MQ=255)                                                        cTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTAATGAACACCTTTCa                                                                                     >  1:342850/1‑141 (MQ=255)                                                        cTTTATCAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCa                                                                                     >  2:337443/1‑141 (MQ=255)                                                           tattaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGCCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTg                                                                                  >  2:424327/1‑141 (MQ=255)                                                           tattaAACAATTCTACCGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTg                                                                                  >  2:122619/1‑141 (MQ=255)                                                            attaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGCATTTCATGCACAACTTTCATTgg                                                                                 >  2:96612/1‑141 (MQ=255)                                                             ttaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGc                                                                                >  2:391111/1‑141 (MQ=255)                                                             ttaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGc                                                                                >  2:263436/1‑141 (MQ=255)                                                              taAACAATTCTACTGTCACTTTTGTACCGTTTGAATGCCGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCa                                                                               <  1:263436/141‑1 (MQ=255)                                                                  cAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTGCATTGGCA‑Cttt                                                                          >  2:579182/1‑141 (MQ=255)                                                                  cAATTCTAATGTCACTTTCGCACCCTCTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAACGATCTTCAATACCTTTCGGCACTTTAAACTCTTGTATTTCATGCACAACTTTCATTGGCA‑Cttt                                                                          <  1:326942/141‑1 (MQ=255)                                                                    tttCTACTGGCACTTTTTTACCGTTTGAATCACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑Cttttt                                                                        <  1:321293/140‑1 (MQ=255)                                                                       cTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCGCAACTTTCATTGGCC‑Ctttttttt                                                                     >  2:7504/1‑141 (MQ=255)                                                                       cTACTGTCAATTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTACTGTGTAATTCTAAACAAATGATCTTCAATACCTTTAGGCGCTTTAAAATCTTGTGTTTCATGCGCAACTTTCATTGGCC‑Ctttttctt                                                                     >  2:109517/1‑141 (MQ=255)                                                                        tACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTg                                                                    >  1:566154/1‑141 (MQ=255)                                                                           tGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTCAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTaa                                                                 <  2:438076/141‑1 (MQ=255)                                                                            ttCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTaaa                                                                <  2:484556/140‑1 (MQ=255)                                                                            gTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTaaa                                                                <  1:575626/141‑1 (MQ=255)                                                                               ccTTTTGTACCGTTTGAATGACGCTATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCa                                                             <  2:561678/140‑1 (MQ=255)                                                                                     gTACCGGTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGC‑‑CCTTTTTTTGTAAATCAATAACTa                                                      >  1:103332/1‑141 (MQ=255)                                                                                        ccGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAACCTTGTCGCGCAGTCACAAATTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTaaa                                                    >  1:441432/1‑141 (MQ=255)                                                                                           tttGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCt                                                 <  2:294744/141‑1 (MQ=255)                                                                                              gAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTa                                              >  2:542692/1‑141 (MQ=255)                                                                                                  gACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATAc                                         <  2:344804/141‑1 (MQ=255)                                                                                                   aCGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTTTTTTTCAATAACTAAAACTTTA‑AAAcc                                        >  2:174746/1‑141 (MQ=255)                                                                                                      cAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTTTTATTTTTTAACTAAATCTTTA‑ATACCCTc                                     >  2:544991/1‑141 (MQ=255)                                                                                                       aaTGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCa                                    >  1:193380/1‑141 (MQ=255)                                                                                                         tgtgTTCGAATTTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CCTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCAcc                                  <  2:43416/141‑1 (MQ=255)                                                                                                            aTTCGAAATTGCTTCCTGTGTAATTCTGAACAGATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTaa                               >  1:437459/1‑141 (MQ=255)                                                                                                            aTTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATACCTACATCTTTA‑ATACCCTCACCTaa                               >  2:609724/1‑141 (MQ=255)                                                                                                            aTTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTaa                               <  2:374119/141‑1 (MQ=255)                                                                                                            aTTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTaa                               >  1:108555/1‑141 (MQ=255)                                                                                                                gAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGAt                           <  1:187133/141‑1 (MQ=255)                                                                                                                    ttGCTTCCTGTTTAATTCTCATCAAATGAGCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTGCATGGACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCGCCTAAAGATTTGt                       <  2:155371/141‑1 (MQ=255)                                                                                                                      gCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATCCCCTCACCTAAAGATTTGTCt                     >  2:498364/1‑141 (MQ=255)                                                                                                                      gCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAACGATTTGTCt                     >  1:108282/1‑141 (MQ=255)                                                                                                                        ttCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCttt                   >  1:211066/1‑141 (MQ=255)                                                                                                                          ccTGTGTAAGTCTTCACAATTGATCTTCAATACCTTTAGGCACTTAAACATCTAATATATCGTTCACAGCTTTCATTGTCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGACTTGTCTTTTa                 <  2:489621/141‑1 (MQ=255)                                                                                                                           cTGTGTACTTCCGAACCACTGCTCTTTAATCCCTTTAGGCACTTTAAAATCTTGGATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTaa                <  2:476190/141‑1 (MQ=255)                                                                                                                           cTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑C‑TTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTaaa               <  2:192744/141‑1 (MQ=255)                                                                                                                                 taTTCTGAATAAAACATTTTTAACTCCTTTAGGCAATTTAAAATCTTGTATTTAATGAACAACTTTCATTGGCACCTTTTTTTTGTAAATCAATAACTAAATCTTTACATTCCGT‑ACCTACAGATTTGTCTTTTAAACCaa           <  2:102841/140‑1 (MQ=255)                                                                                                                                  aTTCTGAACAACTGATCGTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACCACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCCAAAGCTTTGTCTTTTAAACCAAGc         <  1:314345/141‑1 (MQ=255)                                                                                                                                    tCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCgg       >  2:567873/1‑141 (MQ=255)                                                                                                                                    tCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCgg       <  1:243125/141‑1 (MQ=255)                                                                                                                                      tGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCCTTTAAACCAAGCGGTc     <  1:305092/141‑1 (MQ=255)                                                                                                                                       aaacaacTGATCTTCAATACCTTTAGCCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCt    <  2:119706/140‑1 (MQ=255)                                                                                                                                         aCAAATTATCCTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGAACACCTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCTTa  <  1:201222/141‑1 (MQ=255)                                                                                                                                         aCAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTTTTTTTTTTTATCTAAATCTTTA‑ATACCCTCACCTAAAGTTTTGTTTTTTAAACTAAGCAGTCTTa  >  2:518982/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                           TCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCA‑CTTTTTTTTGTAAATCAATAACTAAATCTTTA‑ATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCTTA  >  USA300TCH1516_ALE/2027051‑2027327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑