| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,279,705 | G→A | 100% | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,279,705 | 0 | G | A | 99.8% | 221.7 / ‑7.1 | 76 | A943V (GCA→GTA) | ebh_3 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (26/47); total (27/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT > USA300TCH1516_ALE/2279567‑2279841 | gTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTgat > 1:267466/1‑139 (MQ=255) ttctgCCGTTATTAGTTGATTCTACTTCTGGTGTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTATAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg < 2:344531/138‑1 (MQ=255) ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTGATATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg > 2:556346/1‑141 (MQ=255) gtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgtt > 2:568800/1‑141 (MQ=255) gCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttgt < 1:14139/141‑1 (MQ=255) gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCA‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc < 2:391563/141‑1 (MQ=255) ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCGATTGTCGGACTGTTTGCTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGGTGTTACTGTTGTTTCa > 2:37206/1‑141 (MQ=255) aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACGGTTGTTTCACCTGt < 1:338460/141‑1 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt > 1:586297/1‑141 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt < 2:226749/141‑1 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt < 1:188909/141‑1 (MQ=255) gTTCATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt < 2:340709/141‑1 (MQ=255) ttGATTCTACTTCTGGTTTTCTAGTTACACCTTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTTTTATTAGCT‑TGCGGTTTATCATTCGCATCACCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 2:304298/141‑1 (MQ=255) tGATTCTACTTCCGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc < 1:39041/141‑1 (MQ=255) ttCTACTTCTTGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc < 2:129819/141‑1 (MQ=255) aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATcc > 2:148535/1‑140 (MQ=255) cTTCTGGTTTACTAGTTACATTGTTACCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAAATGTTACTAGCT‑TGCGGCTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCTCCTGTTGCCGCATCAc < 1:446249/141‑1 (MQ=255) tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtt > 1:223786/1‑140 (MQ=255) cTGGTTTACTAGTTACTGCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat < 2:30934/141‑1 (MQ=255) tttATTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTattatt < 2:565872/141‑1 (MQ=255) ttACTTGTTACATCGTTATCCGTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt < 2:222843/141‑1 (MQ=255) ttAGTTACATCGCTGTCCATTTGCGGACTGTTTGTTGATGCATCTACCCTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt < 2:550369/140‑1 (MQ=255) cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt < 1:218043/141‑1 (MQ=255) cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt < 1:544367/141‑1 (MQ=255) tgTTACATTGTTTTCTCTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtgt < 2:47916/140‑1 (MQ=255) gTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGtgtt > 1:486442/1‑141 (MQ=255) gtACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttg < 1:584822/140‑1 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTACCTCTGC‑TATGATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag < 1:443872/141‑1 (MQ=255) gTTATTCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga < 2:1959/141‑1 (MQ=255) gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTGTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga < 1:411471/141‑1 (MQ=255) tATCCATTGTCGGACTGTTTGATGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATAATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTAAGCTGTTGCCGCCTCACTATCTTTTGGTGGTGTCGGAGAAg > 2:420055/1‑141 (MQ=255) ttCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGGATGGTTATTAGCT‑TGCGGTTTATCATGTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc < 2:163382/140‑1 (MQ=255) aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGGGAAGc > 1:82765/1‑141 (MQ=255) aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAgg > 1:179200/1‑140 (MQ=255) cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTGTCGTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAGCACGATTATTTGGTGTTGTCGGAGAAGCggt > 1:90190/1‑139 (MQ=255) cgACTGTNTGTTGGTGCATCTACACTAGAATTGTTATNAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGNATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg < 2:434743/140‑1 (MQ=255) cTGCTTGTAGATGCATCTACACTACAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa < 1:6029/141‑1 (MQ=255) tgtttgttGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAt > 1:346321/1‑141 (MQ=255) tgttgATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 2:424945/141‑1 (MQ=255) tgttgATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 1:190280/141‑1 (MQ=255) gttgATGCATCTTCAATAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt < 2:288471/141‑1 (MQ=255) ttgATGCATCTACACTACAATAGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTTTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTg > 1:146833/1‑141 (MQ=255) tgATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgt > 2:604433/1‑141 (MQ=255) gCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTCGTCGGCGATAGGTCTGCTTGGCCATTAGCtgttgtt > 2:398093/1‑140 (MQ=255) cATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct > 2:74226/1‑141 (MQ=255) aacTACTCTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc < 1:266623/139‑1 (MQ=255) tctaaccTAGAATTGTTATTAGCT‑TGCGGTTTATCTTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa < 2:377646/135‑1 (MQ=255) tcggctcTATAATTGTTATTAGCT‑TGCGGTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa < 2:301137/135‑1 (MQ=255) cTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg < 1:556346/141‑1 (MQ=255) tACACTAGAATTGTTATTACCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGGCGCATCACTATTATTTGGCGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa < 1:37206/141‑1 (MQ=255) tAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 1:41511/141‑1 (MQ=255) tAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTACTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 1:408123/141‑1 (MQ=255) aTTGTTATTAGCT‑TGCGGTTTATCATTTGCATTGTGAGTTGGTGTTGTTACTTTTATTTCACCTATTTCTTCATCACTATTATTTCCTGTTTTCGGATAATCGTCTTCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg < 2:447199/141‑1 (MQ=255) gTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg > 2:480727/1‑141 (MQ=255) gTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTAGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg > 2:340731/1‑141 (MQ=255) ttaGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGTTGTccc > 2:423837/1‑140 (MQ=255) ttaGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCa < 1:88743/141‑1 (MQ=255) tagttggCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg < 2:609221/135‑1 (MQ=255) t‑tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt < 2:380734/141‑1 (MQ=255) tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt > 1:571716/1‑141 (MQ=255) tgttttATCATTTTCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc < 2:115467/138‑1 (MQ=255) cGGTTTATCATTTGCATCCTAAGGGCCCGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc > 2:588085/1‑141 (MQ=255) tGCATCATCAGTTGCTGATGTTACTGTAGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGTTGTTGCAtt > 2:130702/1‑141 (MQ=255) gCATCATCCGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa < 2:64866/141‑1 (MQ=255) gCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGTTGTTGCATTa > 1:135030/1‑141 (MQ=255) tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa > 2:133105/1‑141 (MQ=255) atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt > 2:318084/1‑141 (MQ=255) caGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGGGTTGCATTAGCATTTg > 1:494070/1‑141 (MQ=255) aGTGGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa < 1:275588/141‑1 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCGTTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt < 2:298385/141‑1 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt < 1:148535/141‑1 (MQ=255) cTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCGCGCGTGGCATTAGCATTTGAATTTg < 1:604433/141‑1 (MQ=255) tGATGTCTCTGTTTTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc < 1:123022/141‑1 (MQ=255) ttGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg < 2:363245/140‑1 (MQ=255) tggTTGCTGTTTCTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg < 2:363350/139‑1 (MQ=255) tGTTACTGTTGTTTCACCTGTTGGCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt < 2:75494/141‑1 (MQ=255) tGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt > 1:539593/1‑141 (MQ=255) | GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT > USA300TCH1516_ALE/2279567‑2279841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |