Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,235,408 Δ1 bp intergenic (‑2175/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,235,4070A.99.5% 896.2 / ‑1.8 193intergenic (‑2174/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (87/105);  minor base C (1/0);  total (88/105)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 201 total aligned reads displayed.

GAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGT  >  NC_000913_3_bme_pgi/4235309‑4235502
                                                                                                  |                                                                                               
gAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCTAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                 >  2:4076168/1‑99 (MQ=255)
gAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGat                                                                                                <  1:2666893/100‑2 (MQ=255)
  aCTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatca                                                                                              <  1:3450273/100‑4 (MQ=255)
    ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatcatc                                                                                           >  1:1608464/1‑100 (MQ=255)
     tttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcg                                                                                          >  2:4601570/1‑100 (MQ=255)
      ttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcg                                                                                          <  2:2293098/99‑1 (MQ=255)
      ttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcg                                                                                          <  1:2376500/99‑1 (MQ=255)
      ttGCCGGTTGTATCGGGCAAGGAAGAGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcg                                                                                          <  1:4406831/99‑1 (MQ=255)
        gCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtc                                                                                        >  2:712791/1‑99 (MQ=255)
        gCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtc                                                                                        >  2:4014093/1‑99 (MQ=255)
        gCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtc                                                                                        >  1:2655385/1‑99 (MQ=255)
        gCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtc                                                                                        >  2:1698111/1‑99 (MQ=255)
        gCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAACTTGATCCtcgtc                                                                                        >  2:3756360/1‑99 (MQ=255)
         ccGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGa                                                                                      <  1:972812/100‑1 (MQ=255)
          cGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGat                                                                                     <  2:2761749/100‑1 (MQ=255)
           ggTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGATGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGata                                                                                    <  2:610675/100‑1 (MQ=255)
           ggTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGata                                                                                    >  1:4264113/1‑100 (MQ=255)
               gTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGt                                                                                 <  1:1880631/99‑1 (MQ=255)
                tATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGatat                                                                                   >  2:3906575/1‑96 (MQ=255)
                tATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAg                                                                               >  2:2751832/1‑100 (MQ=255)
                tATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAg                                                                               >  2:2064692/1‑100 (MQ=255)
                 aTCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATg                                                                                  <  1:3910304/96‑1 (MQ=255)
                 aTCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAgg                                                                              >  1:3295814/1‑100 (MQ=255)
                  tCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAgg                                                                              <  2:2557031/99‑1 (MQ=255)
                    gggCAAGGACGGGGCGAGTGGTCGCGACGCTTGCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGTAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                           <  1:4440601/100‑1 (MQ=255)
                    gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGcc                                                                            <  1:2858824/99‑1 (MQ=255)
                     ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                           >  1:4625360/1‑99 (MQ=255)
                      gCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGa                                                                         >  1:493219/1‑100 (MQ=255)
                          ggaaggGGCGAGTGGTCGCGACGCTTCCATGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAg                                                                     <  1:3759810/100‑1 (MQ=255)
                            aaggGGCGGGTGGTCGCGACGGTTCCACGCAAGGGCTTGTCGCGCAGCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                   <  2:4754122/100‑1 (MQ=255)
                             aggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                  >  1:2461999/1‑100 (MQ=255)
                              ggggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                 >  2:1678701/1‑100 (MQ=255)
                                ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCTCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                               <  2:2861318/100‑1 (MQ=255)
                                ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                >  2:3170889/1‑99 (MQ=255)
                                ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                >  1:207286/1‑99 (MQ=255)
                                ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                               <  2:3266648/100‑1 (MQ=255)
                                ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                               <  1:900991/100‑1 (MQ=255)
                                  cGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                             <  1:287126/100‑1 (MQ=255)
                                  cGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCAGCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                               <  2:393701/98‑1 (MQ=255)
                                     gTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                          <  1:1577300/100‑1 (MQ=255)
                                     gTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                          >  1:3087476/1‑100 (MQ=255)
                                      tGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAtgcc                                                          <  1:1133753/99‑1 (MQ=255)
                                       ggTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccg                                                         >  2:3655437/1‑99 (MQ=255)
                                       ggTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccg                                                         >  2:1647570/1‑99 (MQ=255)
                                        gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                       >  1:1481106/1‑100 (MQ=255)
                                        gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                       <  2:4298968/100‑1 (MQ=255)
                                        gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                       >  2:355655/1‑100 (MQ=255)
                                          cgcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                     >  1:1994194/1‑100 (MQ=255)
                                          cgcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                     >  2:451649/1‑100 (MQ=255)
                                          cgcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                     >  1:253555/1‑100 (MQ=255)
                                           gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                    >  1:2973049/1‑100 (MQ=255)
                                           gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                    >  1:3056202/1‑100 (MQ=255)
                                            cgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                   <  2:1219636/100‑1 (MQ=255)
                                             gACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCAgg                                                  <  1:4188396/100‑1 (MQ=255)
                                                 cTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                              <  2:1044745/100‑1 (MQ=255)
                                                  ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                             >  1:1201372/1‑100 (MQ=255)
                                                   tCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                             >  2:2702988/1‑99 (MQ=255)
                                                   tCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                             >  1:4720886/1‑99 (MQ=255)
                                                     cACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg                                            <  2:2572768/98‑1 (MQ=255)
                                                     cACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                           <  2:4840261/99‑1 (MQ=255)
                                                      aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACCCGATg                                         >  1:3857045/1‑100 (MQ=255)
                                                        gCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                       <  2:305645/100‑1 (MQ=255)
                                                           aGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                     <  2:492987/99‑1 (MQ=255)
                                                             ggCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                   <  2:1142499/99‑1 (MQ=255)
                                                             ggCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                  <  2:2786869/100‑1 (MQ=255)
                                                              gCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                 >  1:3121957/1‑100 (MQ=255)
                                                              gCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                 <  1:2061449/100‑1 (MQ=255)
                                                                  gTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                 <  1:326678/96‑1 (MQ=255)
                                                                   tCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                            >  1:4647253/1‑100 (MQ=255)
                                                                   tCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                            <  1:3341998/100‑1 (MQ=255)
                                                                    cgcgCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                               >  2:2453638/1‑96 (MQ=255)
                                                                    cgcgCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                           >  1:2508650/1‑100 (MQ=255)
                                                                    cgcgCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                           >  2:297214/1‑100 (MQ=255)
                                                                    cgcgCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                           >  2:147262/1‑100 (MQ=255)
                                                                     gcgcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg                          <  1:1817557/100‑1 (MQ=255)
                                                                      cgcATGTGCATGCGCGAAGGAAAGTTTGATCATCGTCGTTATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg                          <  1:4554217/99‑1 (MQ=25)
                                                                       gcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                         >  1:4289378/1‑99 (MQ=255)
                                                                       gcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                        <  1:1269372/100‑1 (MQ=255)
                                                                            tGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                   >  2:4411353/1‑100 (MQ=255)
                                                                              cATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                  <  1:4018043/99‑1 (MQ=255)
                                                                              cATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                  <  1:4668527/99‑1 (MQ=255)
                                                                              cATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                    <  1:3988003/97‑1 (MQ=255)
                                                                                tGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt               >  1:2931828/1‑100 (MQ=255)
                                                                                 gcgcGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt               >  1:698953/1‑99 (MQ=255)
                                                                                  cgcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa             <  2:3337702/100‑1 (MQ=255)
                                                                                  cgcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGATCGCGTCTTATc              <  1:1503964/99‑1 (MQ=255)
                                                                                    cgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg           <  1:3837690/100‑1 (MQ=255)
                                                                                    cgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg            <  2:1542416/99‑1 (MQ=255)
                                                                                      aaGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc         <  1:3401666/100‑1 (MQ=255)
                                                                                      aaGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGca         <  1:1174461/100‑2 (MQ=18)
                                                                                      aaGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGc          <  1:2117962/99‑1 (MQ=18)
                                                                                       aGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGcat        <  1:3658702/100‑3 (MQ=18)
                                                                                        ggAAAGCTTGATCGTCGTCGATATGTGGGCCGGATAAGGCGTTCAGGCCGCGTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc         <  2:2929414/98‑1 (MQ=18)
                                                                                         gAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa       >  2:4218392/1‑99 (MQ=255)
                                                                                         gAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa       >  1:4600082/1‑99 (MQ=255)
                                                                                         gAAAGCTTGATCATCGGCGATATGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCGACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgn           <  2:1376302/95‑2 (MQ=18)
                                                                                          aaaGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa     <  2:4259722/100‑1 (MQ=255)
                                                                                          aaaGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa     <  2:2624484/100‑1 (MQ=255)
                                                                                           aaGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg    >  2:1274535/1‑100 (MQ=255)
                                                                                             gCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt  >  1:3220751/1‑100 (MQ=255)
                                                                                                  |                                                                                               
GAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGT  >  NC_000913_3_bme_pgi/4235309‑4235502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: