| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,233,680 | T→C | intergenic (‑447/‑2227) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,233,680 | 0 | T | C | 98.3% | 190.8 / ‑5.2 | 60 | intergenic (‑447/‑2227) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (34/25); minor base G (0/1); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
GTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCG > NC_000913_3_bme_pgi/4233536‑4233823 | gtaCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAGCAGTACGCTAAGGGCGCCaaaa > 1:443229/1‑149 (MQ=255) aCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAAcc < 1:297447/149‑1 (MQ=255) cATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCa < 1:897660/149‑1 (MQ=255) aTTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCAt < 2:613736/149‑1 (MQ=255) aTTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCAt < 2:169444/149‑1 (MQ=255) aTTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCACCGTGAATTATGATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCAt < 1:534605/149‑1 (MQ=255) tttATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATc < 1:50402/149‑1 (MQ=255) tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCCCCAAAACCATcac > 1:54193/1‑149 (MQ=255) tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATccc > 1:17231/1‑147 (MQ=255) tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATcac < 1:525026/149‑1 (MQ=255) gggTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCtgt < 2:351856/149‑1 (MQ=255) gggTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAACAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCtgt < 2:283652/149‑1 (MQ=255) ggggCGGTTGTCAACGATGGGGTCATGCGGAGTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCACAACCATCACATTTGTCTTTGACgggtg > 2:318410/1‑147 (MQ=255) cAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTc < 2:865981/149‑1 (MQ=255) aaCGATGGGGTCATGCGGATTTTTCATCCGCTCCTGGCGGTCAGAAGTTCCGCTGATTAATGCTTCACTGTGCTAAGGGTTTACACACAACATTACGCTAACGACACCAAAACCATCACATTTTTATGTGACTGGTGCTACAATGTTcc > 1:845396/1‑149 (MQ=255) aaCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGCCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCCACAATCTTcc > 2:858098/1‑149 (MQ=255) gATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCaaa < 1:681660/149‑1 (MQ=255) aTGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAACTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAg < 1:996369/149‑1 (MQ=255) tGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGt > 2:557109/1‑149 (MQ=255) tCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACaa > 2:959535/1‑149 (MQ=255) cGGATTTTTCATCCACTCCTGGCGGACAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTCCAATCTTCCAGATTAACAATtcta > 2:302967/1‑148 (MQ=255) aTTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaa < 1:488669/149‑1 (MQ=255) tttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaaa > 2:46183/1‑149 (MQ=255) ttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa > 2:428084/1‑149 (MQ=255) aTCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATc < 2:539683/92‑1 (MQ=255) aTCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATc > 1:539683/1‑92 (MQ=255) cACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACCTTTATTGAATACCTGTGTGACAATCTTCCAAGAACGCAATTCTCAAAATCAGAagag > 1:976229/1‑149 (MQ=255) cACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCAACAATCTTCCAAAGTCACAAATCTCAAAAACAGAagag > 1:309844/1‑149 (MQ=255) tGGCGGTCGGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATAAGAAGAGTATTGc > 2:27825/1‑149 (MQ=255) tGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGc > 2:658065/1‑149 (MQ=255) gTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATg < 2:165802/149‑1 (MQ=255) atCAGTAGGTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATg < 1:221222/148‑1 (MQ=255) tagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAag > 1:630181/1‑149 (MQ=255) tagtTCAGCTAATAAATGCTGCACTGCGCTAAGGTTTTACACTCAACATTACGCTAACGGCACCACAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCATAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAag < 2:443229/149‑1 (MQ=255) gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAagag > 1:702211/1‑149 (MQ=255) ttCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAgaga > 1:848708/1‑149 (MQ=255) gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGAcgcg > 2:867417/1‑149 (MQ=255) ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGCCGccacaa > 2:996933/1‑147 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGc < 1:959535/149‑1 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAGATCAGAAGAGTATTGTCA‑‑ATGGCAAGAGATGCGACGAAGCTTGaa < 2:181782/149‑1 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGaa > 2:630179/1‑149 (MQ=255) tGCGCTAAGGGTTTACACTGAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAAc > 2:319882/1‑149 (MQ=255) gggTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTTCTA‑‑ATGGCAAGATACGCGACGAAGCTTGAAGCAACGGTCGCaa > 2:491993/1‑149 (MQ=255) ggTTGACACTCAACATTACGTTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCaaa < 2:378441/149‑1 (MQ=255) tttACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAACGACACCAATCTCAACAT‑‑GAATGGCAGTACCAGCAGGGGATGAGAGGCGACGAAGCTTGACGCAACGGTCGCagatc > 1:444572/1‑145 (MQ=255) cacTCAACATTACGCTATCGGCTCGAAGACCATCACATTTTTCTGTGACTGGTGGTTCAATCTTCCAAAGTCGCAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCTAAGCTGaa < 2:845396/149‑1 (MQ=255) acTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc < 2:198987/144‑1 (MQ=255) acTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc > 1:198987/1‑144 (MQ=255) cttaaCATTACGCTAACGGCACCAAAACCATCACATGTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAACCCCAAAAAGCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaa < 2:418431/146‑1 (MQ=255) tCAACATTTCGCTAACGGCACCAAACCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa < 1:179536/149‑1 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa > 1:804889/1‑149 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGATTGAACCAACGGTCGCAAAGCTGaaaaa > 2:578824/1‑149 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTATCAAAATCAGAGGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGACGAAACGGTCGCAAAGATggcaga > 2:23733/1‑144 (MQ=255) aaCATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc > 2:448676/1‑149 (MQ=255) ttACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAg > 2:135550/1‑114 (MQ=255) ttACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAg < 1:135550/114‑1 (MQ=255) gCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCGAAGCTGAAAAAGCATTGGGCg > 1:764003/1‑149 (MQ=255) tAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTACAAAGTCACAAGTCTAAAAATTAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGAAACGCTGAAAAAGCAttagcggga > 2:166051/1‑142 (MQ=255) cGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc < 1:587199/149‑1 (MQ=255) ggCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg > 1:617681/1‑149 (MQ=255) | GTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCG > NC_000913_3_bme_pgi/4233536‑4233823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |