| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,233,708 | C→T | intergenic (‑475/‑2199) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,233,708 | 0 | C | T | 98.3% | 184.5 / ‑4.3 | 59 | intergenic (‑475/‑2199) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (37/21); total (37/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
CAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGC > NC_000913_3_bme_pgi/4233572‑4233853 | cAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTc < 2:865981/149‑1 (MQ=255) aaCGATGGGGTCATGCGGATTTTTCATCCGCTCCTGGCGGTCAGAAGTTCCGCTGATTAATGCTTCACTGTGCTAAGGGTTTACACACAACATTACGCTAACGACACCAAAACCATCACATTTTTATGTGACTGGTGCTACAATGTTcc > 1:845396/1‑149 (MQ=255) aaCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGCCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCCACAATCTTcc > 2:858098/1‑149 (MQ=255) gATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCaaa < 1:681660/149‑1 (MQ=255) aTGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAACTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAg < 1:996369/149‑1 (MQ=255) tGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGt > 2:557109/1‑149 (MQ=255) tCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACaa > 2:959535/1‑149 (MQ=255) cGGATTTTTCATCCACTCCTGGCGGACAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTCCAATCTTCCAGATTAACAATtcta > 2:302967/1‑148 (MQ=255) aTTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaa < 1:488669/149‑1 (MQ=255) tttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaaa > 2:46183/1‑149 (MQ=255) ttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa > 2:428084/1‑149 (MQ=255) cACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACCTTTATTGAATACCTGTGTGACAATCTTCCAAGAACGCAATTCTCAAAATCAGAagag > 1:976229/1‑149 (MQ=255) cACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCAACAATCTTCCAAAGTCACAAATCTCAAAAACAGAagag > 1:309844/1‑149 (MQ=255) tGGCGGTCGGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATAAGAAGAGTATTGc > 2:27825/1‑149 (MQ=255) tGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGc > 2:658065/1‑149 (MQ=255) gTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATg < 2:165802/149‑1 (MQ=255) atCAGTAGGTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATg < 1:221222/148‑1 (MQ=255) tagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAag > 1:630181/1‑149 (MQ=255) tagtTCAGCTAATAAATGCTGCACTGCGCTAAGGTTTTACACTCAACATTACGCTAACGGCACCACAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCATAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAag < 2:443229/149‑1 (MQ=255) gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAagag > 1:702211/1‑149 (MQ=255) ttCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAgaga > 1:848708/1‑149 (MQ=255) gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGAcgcg > 2:867417/1‑149 (MQ=255) ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGCCGccacaa > 2:996933/1‑147 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGc < 1:959535/149‑1 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAGATCAGAAGAGTATTGTCA‑‑ATGGCAAGAGATGCGACGAAGCTTGaa < 2:181782/149‑1 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGaa > 2:630179/1‑149 (MQ=255) tGCGCTAAGGGTTTACACTGAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAAc > 2:319882/1‑149 (MQ=255) gggTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTTCTA‑‑ATGGCAAGATACGCGACGAAGCTTGAAGCAACGGTCGCaa > 2:491993/1‑149 (MQ=255) ggTTGACACTCAACATTACGTTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCaaa < 2:378441/149‑1 (MQ=255) tttACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAACGACACCAATCTCAACAT‑‑GAATGGCAGTACCAGCAGGGGATGAGAGGCGACGAAGCTTGACGCAACGGTCGCagatc > 1:444572/1‑145 (MQ=255) cacTCAACATTACGCTATCGGCTCGAAGACCATCACATTTTTCTGTGACTGGTGGTTCAATCTTCCAAAGTCGCAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCTAAGCTGaa < 2:845396/149‑1 (MQ=255) acTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc > 1:198987/1‑144 (MQ=255) acTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc < 2:198987/144‑1 (MQ=255) cttaaCATTACGCTAACGGCACCAAAACCATCACATGTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAACCCCAAAAAGCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaa < 2:418431/146‑1 (MQ=255) tCAACATTTCGCTAACGGCACCAAACCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa < 1:179536/149‑1 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa > 1:804889/1‑149 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGATTGAACCAACGGTCGCAAAGCTGaaaaa > 2:578824/1‑149 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTATCAAAATCAGAGGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGACGAAACGGTCGCAAAGATggcaga > 2:23733/1‑144 (MQ=255) aaCATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc > 2:448676/1‑149 (MQ=255) ttACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAg < 1:135550/114‑1 (MQ=255) ttACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAg > 2:135550/1‑114 (MQ=255) gCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCGAAGCTGAAAAAGCATTGGGCg > 1:764003/1‑149 (MQ=255) tAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTACAAAGTCACAAGTCTAAAAATTAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGAAACGCTGAAAAAGCAttagcggga > 2:166051/1‑142 (MQ=255) cGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc < 1:587199/149‑1 (MQ=255) ggCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg > 1:617681/1‑149 (MQ=255) accaaaaCCATCACATTTTTTTTTTTTTTTTGCTACAATCTTCCAAAGTCACAATTCTAAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGATACGCGACGAAGCTTGAAGCAACGGTCTTATAGCTGTAAATTCATTGGGCGGAATCGGcc > 2:418960/4‑149 (MQ=255) aaaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACTAAGCACAAAGCAACGGTCGCAAGGCTGAAAAAGCATTGGGCGGAATCGGccccc > 1:693107/1‑148 (MQ=255) aaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGACCCcgc > 2:211890/1‑149 (MQ=255) cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa < 1:106156/149‑1 (MQ=255) cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa > 1:180973/1‑149 (MQ=255) cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGCATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa < 2:804889/149‑1 (MQ=255) aTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTGTTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat > 1:868997/1‑149 (MQ=255) aTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat < 2:17231/149‑1 (MQ=255) tCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAATATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCCGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata < 2:947209/149‑1 (MQ=255) tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATAAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc < 2:54193/149‑1 (MQ=255) ttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGcgcgcg < 1:46183/149‑1 (MQ=255) cTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGc > 2:33135/1‑149 (MQ=255) tGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgc > 2:574728/1‑149 (MQ=255) tGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAACTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgc > 1:264107/1‑149 (MQ=255) | CAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑ATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGC > NC_000913_3_bme_pgi/4233572‑4233853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |