Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913_3_bme_pgi | 4,233,708 | C→T | intergenic (‑475/‑2199) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913_3_bme_pgi | 4,233,708 | 0 | C | T | 98.0% | 146.9 / NA | 49 | intergenic (‑475/‑2199) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (29/19); minor base G (0/1); total (29/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCA > NC_000913_3_bme_pgi/4233564‑4233847 | ccGGTTGTCAACGGTGGGGTCAAGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTCCACTCAACATTACGCAAACGACACCAAAACTATCACATTTTTCTGTTACTGgtgcaa > 2:195827/2‑147 (MQ=255) aTGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACAGTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAAtt < 1:73684/149‑1 (MQ=255) cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTCATACATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtctc < 1:20496/149‑1 (MQ=255) gATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaa > 1:390179/1‑149 (MQ=255) tttttCACCCAGTCCTCGCGGTCAGGAGTTCAGCTAATAAAGGCTTCACTGCGCTAAGGGTTTACACTCAACATGACGGTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAGGTCACAATTCTCaaat < 2:195576/149‑2 (MQ=255) tCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaag > 2:611321/1‑149 (MQ=255) ccACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaaga > 1:124880/1‑149 (MQ=255) aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGt > 1:711408/1‑149 (MQ=255) ccTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTAtt < 2:350619/149‑1 (MQ=255) ggcAGTAGTTCATCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGGCACAATTCTCAAAATCAGAAGAGTATTGCTAATg < 2:359051/147‑1 (MQ=255) gTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATg > 1:508730/1‑149 (MQ=255) tCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTAAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATgg > 1:764675/1‑149 (MQ=255) cAGTAGTTCAGCAAATAAAAGCTGCACCGCTCTAAGGGTTTACACTCAACATCACGCTAACGGCACCAAAACCAACACCTTTTACTGTGACTGGTGCTACAATCTTCCAAAGTCACAATCCTCAAAATCAGAAGAGTATTGCTAATGGc < 2:6656/149‑1 (MQ=255) gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATGCTCAAAATCAGAAGAGTATTGCTAATGGCaa < 2:599675/149‑1 (MQ=255) ggtgtTCAGCTAATAAATGCTTCACTGCGCGAAGGGTTTACACTCTACATTACGCTAACGGCACCAAAACCATCCCAGTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCaa < 2:357181/146‑1 (MQ=255) gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTTCTAATGGCAagag > 1:159310/1‑149 (MQ=255) aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGCCTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATACGAAAAGTATTGCTACCGGCAAAAGAcgc > 2:679358/1‑149 (MQ=255) aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGAcgc > 1:151997/1‑149 (MQ=255) cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGa < 2:52018/149‑1 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCt > 1:648145/1‑83 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCt < 2:648145/83‑1 (MQ=255) agcTACGCGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACgg < 1:346260/148‑1 (MQ=255) gggTTTACACTCAACATTACGCTAACGGCCCCACCATCATCACATTTTTGTCTGCCTGGTCATCAACTCTTCAACAGTTACAACTCTGAAAATCAGAAGAGTTGTGCTAATTGCCAGAGACGCGGCTAAGCGTGATGCAACGGTCGCaa > 2:678168/1‑149 (MQ=255) tACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATAGCAAGAGACGCGACGACGCTTGAAGCAACGGTCGCAAAGCTg > 1:85632/1‑149 (MQ=255) acTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaa > 1:153308/1‑149 (MQ=255) cTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATTGCAAGAGACGCGACGAAACTTGAAGCAACGGTCGCAAAGCTGaaaa > 2:61111/1‑149 (MQ=255) cTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAAGGAAAGAGACGCGACGAAGCTTGAAGCAACGGGCGCAAAGCTGaaaa > 2:589168/1‑149 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa > 1:499433/1‑149 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa > 1:483466/1‑149 (MQ=255) cAACATAATCCTAATGGCACCAAAACCATCACATTTATCTTTGAGCAAGACTACAATCTTCCAAAGTCACAATTCGCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAATCTTGAAGCAACGGTCGCAAAGCTGAAAAAg < 2:85632/149‑1 (MQ=255) aaCATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc > 1:206949/1‑149 (MQ=255) aaCATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTCAAGCAACGGTCGCAACGCAGAAAAAGc > 2:464837/1‑149 (MQ=255) aCATTACGCCAACGGCACCAAAACCATCACATTTTGCTGTTACTGGTGCTAAAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTAGTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCa < 2:328988/149‑1 (MQ=255) ttACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAACCATTg > 2:646251/1‑149 (MQ=255) taagatAACGGTACCAAAACCATGACATTTTTCTGTGAATGGTGATAAAATAATCCAAGGTGAAAATGCTCAAAACCAGTAGAGTATTGCTAATGGCAAGAGACGCGACGAGGCTTTTTGCAACGGTAAAAAAAAAAAAAAAGCATTgg < 2:114674/144‑1 (MQ=255) aCGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAAGCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAGCGGTCGCAAAGCTGAAAAAGCATTggg < 1:195827/149‑1 (MQ=255) gCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATATTCCAAAGTCACAAATCTAAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGCCGCAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTTGGCg > 1:711904/1‑149 (MQ=255) aaCGGCACCAAAACCATCACATATTAATAAAAATGGTGCTACAATCTGCCAAAGTCACAAATATCAAAACCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACAAAAAAAAAAAAAAAAAAGCATTGGGCGGaa < 2:711408/149‑1 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGTGACTAGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCGACGAAGATTGAAGCACAGGTCGCAAAGCTGAAAAAGCATTGGGCGAAAt > 2:22291/1‑149 (MQ=255) ggCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCAAAAGTCACAATTCTAAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCAACGAAGCTTGAAGCAACGGTAGCAACACTGACAACGACTTAGGCGGAATCg > 2:320611/1‑149 (MQ=255) aaaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAACAGTGCGCGGAATCGGCCCcg > 2:808793/1‑149 (MQ=255) aaaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTACAACAGACTTGGGCGGAATCGGCCCcg > 2:808165/1‑149 (MQ=255) cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa < 1:454548/149‑1 (MQ=255) tCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata > 1:443200/1‑149 (MQ=255) cacaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGatat < 2:483466/149‑1 (MQ=255) acaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAACGCATTGGGCGGAATCGGCCCCGCGCGATATg > 1:48777/1‑149 (MQ=255) caTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc > 1:692342/1‑149 (MQ=255) caTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATga > 2:108408/1‑148 (MQ=255) tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc < 1:528554/149‑1 (MQ=255) tgtgACTGGTGCTACAATCTACCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa < 1:209678/149‑1 (MQ=255) | GCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCA > NC_000913_3_bme_pgi/4233564‑4233847 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |