| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,233,680 | T→C | intergenic (‑447/‑2227) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,233,680 | 0 | T | C | 98.0% | 161.2 / ‑6.8 | 50 | intergenic (‑447/‑2227) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (32/17); minor base A (1/0); total (33/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGG > NC_000913_3_bme_pgi/4233537‑4233824 | taCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGCTTTACACTCAACATTACGCTCACGGCACCAAAAc > 2:733702/1‑149 (MQ=255) aCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAAAATTACGCTAACGGCACCAAAAcc > 2:714398/1‑149 (MQ=255) aTTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAAGAAATGCTTCACTGCGCTAAGGGTTTAAACTCAACATTCCGCTGACGGGACCGAAAcact > 1:300268/1‑146 (MQ=255) aTTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATACACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACACAAACCAt > 2:479738/1‑149 (MQ=255) aTTTAGAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAAACAt < 2:377985/149‑1 (MQ=255) ttATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCGAAACCATca > 2:103315/1‑149 (MQ=255) gggTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCtgt > 1:367599/1‑149 (MQ=255) cgCGGGGCGGTTTTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTg < 1:799148/148‑1 (MQ=255) ggTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTGCa > 1:255841/1‑149 (MQ=255) tCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCtt > 2:775416/1‑149 (MQ=255) cAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTc < 2:537910/149‑1 (MQ=255) gATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGAGCTAAGGGTTTATACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCaaa < 2:721904/149‑1 (MQ=255) tGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGt > 2:62383/1‑149 (MQ=255) gggTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTca < 1:450412/149‑1 (MQ=255) gTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAACGTcaca > 1:128341/1‑149 (MQ=255) gTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTcaca > 1:131229/1‑149 (MQ=255) aTGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGATCGGGGTGTACACTCAACATTACGCTAACGGCACCAAAACCAGCACATTTTTCTGTGACTGGTGCTACAAGCTTCCAAAGTCACAAtg > 1:491768/1‑148 (MQ=255) cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtctc > 2:793215/1‑149 (MQ=255) tttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTAACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTCCAATCTTGCAAAGTCACAATTCTCaaaa > 1:764409/1‑149 (MQ=255) ttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAAt < 1:62383/149‑1 (MQ=255) ttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa < 1:501090/149‑1 (MQ=255) tCCACTACTGGCGGTCACTAGTTCAGCTAATAAATGCTTCACTTCGCTAAGGGTTTACACTCAACATGATGCTAACGGCACCAAAACCATCACGTTTTTCTGTGACTGGTGCTAGAATCTTCCAAAGTGACAAGTCTCAAAAGCagaag > 2:686454/1‑149 (MQ=255) ccACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaaga < 1:219055/149‑1 (MQ=255) ccACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaaga < 1:392832/149‑1 (MQ=255) aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCAAAATTCTCAAAATCAGAAGAGt > 1:472361/1‑149 (MQ=255) cTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTg > 2:145453/1‑149 (MQ=255) tGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGAATTGc > 2:527178/1‑149 (MQ=255) gCGGTCAGTAGTTCAGATAATAAATGCTTCACTGCGTTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa < 1:670667/149‑1 (MQ=255) cAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGAcg > 2:563935/1‑149 (MQ=255) cgCTCATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGAcgc < 1:11790/148‑1 (MQ=255) cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga > 1:437988/1‑149 (MQ=255) tGCTTCACTGCGATAAGGATTTACAATCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTCTAATCTTCCAAAGTCACAATTCTCAAAACTGGAGTAGTATTACTAATGGCAAGAGACGCGACGAAGCtt < 2:142712/149‑1 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa < 1:810203/149‑1 (MQ=255) gcTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAaga < 1:287976/123‑1 (MQ=255) gcTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAaga > 2:287976/1‑123 (MQ=255) gcTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGt > 2:364191/1‑149 (MQ=255) tAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGtag > 1:336518/1‑147 (MQ=255) gggTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCACAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACAGTAGCaa > 2:232030/1‑149 (MQ=255) gTTTACACTCAACATTACGCTAACGGCCCCCACTCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTACTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAg > 2:391846/1‑149 (MQ=255) gTTTACACTCAACATGACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAg < 2:524610/149‑1 (MQ=255) tttACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGACGCTTGAAGCAACGGTAGCaagcc > 2:205867/1‑146 (MQ=255) cacTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGaa < 2:540508/149‑1 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga < 2:526336/115‑1 (MQ=255) tCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga > 1:526336/1‑115 (MQ=255) gCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCGTGAAGCAACGATAGCAAAGCTGAAAAAGCATTGGGCg > 2:36610/1‑149 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAAt > 1:82942/1‑149 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAAt > 2:785882/1‑149 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGAGACTGGTGCCACCATCGTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGTCGAAGCTTGAAGCAACGGTAGCATAGCTGAACCAGCATTGGGCGGagt > 1:32544/1‑147 (MQ=255) ggCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCCACGCAGCTTGAAGCAACGATCGCAACGCTGAAAAAGCATGTGCCGGAATCg > 2:227051/1‑149 (MQ=255) gCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCgg > 1:149871/1‑149 (MQ=255) | TACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGG > NC_000913_3_bme_pgi/4233537‑4233824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |