Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,746 A→G intergenic (‑513/‑2161) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7460AG99.5% 756.7 / NA 207intergenic (‑513/‑2161)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (103/103);  total (104/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCG  >  NC_000913_3_bme_pgi/4233649‑4233842
                                                                                                 |                                                                                                
    ttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCACAATCAGAGGAGTAt                                                                                            >  2:2129042/1‑100 (MQ=255)
       cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGc                                                                                         >  1:5707847/1‑100 (MQ=255)
       cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCCTCCAAACTCACAATTCTCAAAACCAgaggag                                                                                               >  2:2020060/1‑94 (MQ=255)
        acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGc                                                                                         >  1:3868696/1‑99 (MQ=255)
         cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCt                                                                                        >  2:1262818/1‑99 (MQ=255)
         cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTa                                                                                       <  1:3158165/100‑1 (MQ=255)
            aaCATTACGCTAGCGGCACTAAAACCATCACATTTTTCTGTGACTGGCTCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAAt                                                                                     <  1:5057906/99‑1 (MQ=255)
              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATgg                                                                                   >  1:5347126/1‑99 (MQ=255)
              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATgg                                                                                   >  1:4433972/1‑99 (MQ=255)
              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATgg                                                                                   >  1:382662/1‑99 (MQ=255)
              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATgg                                                                                   >  1:3754751/1‑99 (MQ=255)
              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATgg                                                                                   >  2:5292492/1‑99 (MQ=255)
              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGc                                                                                  <  1:4700690/100‑1 (MQ=255)
               aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCa                                                                                 <  1:3524588/100‑1 (MQ=255)
                ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGc                                                                                  >  2:648478/1‑98 (MQ=255)
                ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGc                                                                                  >  1:3330384/1‑98 (MQ=255)
                  aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag                                                                               <  1:3560828/99‑1 (MQ=255)
                  aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag                                                                               <  2:4308140/99‑1 (MQ=255)
                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCaa                                                                                >  2:78933/1‑97 (MQ=255)
                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCaa                                                                                >  1:1609954/1‑97 (MQ=255)
                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCaa                                                                                >  2:46489/1‑97 (MQ=255)
                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAagag                                                                             <  2:504670/100‑1 (MQ=255)
                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAagag                                                                             <  2:3857707/100‑1 (MQ=255)
                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAagag                                                                             <  1:4463184/100‑1 (MQ=255)
                    gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag                                                                               <  1:5157729/97‑1 (MQ=255)
                    gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAgaga                                                                            >  2:2825131/1‑100 (MQ=255)
                     cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAc                                                                           >  1:793617/1‑100 (MQ=255)
                         cGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGcga                                                                       <  1:5467919/100‑1 (MQ=255)
                             aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGaa                                                                   <  2:4372366/100‑1 (MQ=255)
                              cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGCGTATTGCTAATGGCAAGAGACGCGACGTAg                                                                  >  1:5635595/1‑100 (MQ=255)
                              cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGTGACGAAg                                                                  <  2:5960664/100‑1 (MQ=255)
                              cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAg                                                                  <  2:1189199/100‑1 (MQ=255)
                              cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAg                                                                  >  1:1332642/1‑100 (MQ=255)
                                  aaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTg                                                              <  1:720423/100‑1 (MQ=255)
                                   aCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGa                                                             <  1:3257628/100‑1 (MQ=255)
                                     cATCACATTTTTCTTTTTTTTTCTCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaat                                                           >  2:376904/1‑99 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTTCTAATGGCAAGAGACGCGACGAAGCTTg                                                              >  2:3930220/1‑97 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGGGACGCGACGAAGCTTGa                                                             <  2:1333898/98‑1 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                            <  1:5932306/99‑1 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                           <  1:1098321/100‑1 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                           <  1:1926547/100‑1 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                           <  2:5637656/100‑1 (MQ=255)
                                     cATCACATTTTTCTGTGACTGGCGCTACAATCGTCCAAAGTCACAATTGTCACAATCAGGGGAGTGTTGTTAGTGGCAAGGGACGCGACGAAGCTTGAAg                                                           <  2:4663572/100‑1 (MQ=255)
                                      aTCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGc                                                          <  2:5106467/100‑1 (MQ=255)
                                       tCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGc                                                          >  1:5680139/1‑99 (MQ=255)
                                           aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACgg                                                     <  2:5942213/100‑1 (MQ=255)
                                            tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGt                                                    >  1:894714/1‑100 (MQ=255)
                                              tttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCg                                                  >  1:2678190/1‑100 (MQ=255)
                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAg                                             <  2:3198082/99‑1 (MQ=255)
                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAg                                             <  2:5676424/99‑1 (MQ=255)
                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                            <  1:237643/100‑1 (MQ=255)
                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                            <  1:4203833/100‑1 (MQ=255)
                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                            >  2:2879375/1‑100 (MQ=255)
                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                            <  2:1070849/100‑1 (MQ=255)
                                                     gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCt                                           >  2:461562/1‑100 (MQ=255)
                                                      aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCt                                           <  2:4284402/99‑1 (MQ=255)
                                                          gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaa                                        <  1:4676341/98‑1 (MQ=255)
                                                            gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    >  1:4971112/1‑100 (MQ=255)
                                                            gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    >  2:5263384/1‑100 (MQ=255)
                                                            gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    <  2:4019164/100‑1 (MQ=255)
                                                             cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    >  1:1801995/1‑99 (MQ=255)
                                                              tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    >  2:2109761/1‑98 (MQ=255)
                                                                cactctcCCAACGTCACAATACTCAAAATCAGAGGAGTAGTACTAATCGCAAGGGACGCGGCGAAGCTTGAAGCAACGGTCGCAAAGGTGAAAAAGCAtt                                <  2:3334392/97‑1 (MQ=255)
                                                                cAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCa                                  <  1:4885802/98‑1 (MQ=255)
                                                                cAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                >  1:4002630/1‑100 (MQ=255)
                                                                cAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAt                                 <  1:1477515/99‑1 (MQ=255)
                                                                 aaTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                               >  2:557795/1‑100 (MQ=255)
                                                                 aaTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                               >  1:2070953/1‑100 (MQ=255)
                                                                  aTCTTCCAAAGTCACAATTCTCACAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                              <  1:5762228/100‑1 (MQ=255)
                                                                  aTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                              >  2:3567748/1‑100 (MQ=255)
                                                                  aTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                              >  2:577025/1‑100 (MQ=255)
                                                                    cTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTggg                             >  2:2509199/1‑99 (MQ=255)
                                                                       ncAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                          >  2:4862983/2‑99 (MQ=255)
                                                                       ccAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGc                            <  1:1272335/97‑1 (MQ=255)
                                                                        cAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                          >  2:116329/1‑98 (MQ=255)
                                                                          aaGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAGCATTGGGCGGAATCg                     >  2:1011835/1‑100 (MQ=255)
                                                                           aGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                     <  2:2186144/100‑1 (MQ=255)
                                                                           aGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                     <  2:1661011/100‑1 (MQ=255)
                                                                           aGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                     <  1:2911326/100‑1 (MQ=255)
                                                                            gTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCAAAAAGATGAAAAAGCATTGGGCGGAATCgg                    <  2:869024/100‑1 (MQ=255)
                                                                             tCACGATTCTAAAAAACAGAGGAATATTGCTAATGGCAAGAGAAGCGACGAAGCTTGAAGCAACGGTAAAAAAAAAAAAAAAGCATTGGGCGGAATCGGc                   <  2:1452934/100‑1 (MQ=255)
                                                                             tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGAAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGc                   <  2:2944700/100‑1 (MQ=255)
                                                                               acaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                  <  1:3942210/99‑1 (MQ=255)
                                                                                caATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGATGAAAAAGCATTGGGCGGAATCGGccc                 <  2:3126846/99‑1 (MQ=255)
                                                                                  aTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgc              >  1:2409840/1‑100 (MQ=255)
                                                                                  aTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgc              >  2:623731/1‑100 (MQ=255)
                                                                                    tctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  1:1222570/1‑100 (MQ=255)
                                                                                    tctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  1:5597844/1‑100 (MQ=255)
                                                                                     ctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  1:216645/1‑99 (MQ=255)
                                                                                     ctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  1:531400/1‑99 (MQ=255)
                                                                                     ctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  2:4052417/1‑99 (MQ=255)
                                                                                     ctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            <  1:4028506/99‑1 (MQ=255)
                                                                                      tcAAAATCAGAGGAGTATTGCTAATGGCAAGGGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  1:2953542/1‑100 (MQ=255)
                                                                                      tcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  1:4834532/1‑100 (MQ=255)
                                                                                       cAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  2:3166605/1‑99 (MQ=255)
                                                                                        aaaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgcg           <  1:347064/97‑1 (MQ=255)
                                                                                        aaaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgcg           <  1:754180/97‑1 (MQ=255)
                                                                                        aaaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat         <  2:5317181/99‑1 (MQ=255)
                                                                                             cAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc   >  1:4792756/1‑100 (MQ=255)
                                                                                              agaggaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg  <  1:999640/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCG  >  NC_000913_3_bme_pgi/4233649‑4233842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: