New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	3746905 =	60 (1.350)	4 (0.090)	3/272	6.5	7.0%	coding (812/987 nt)	yiaO	2,3‑diketo‑L‑gulonate‑binding periplasmic protein
	?	NC_000913_3_bme_pgi	3746922 =	50 (1.180)					coding (829/987 nt)	yiaO	2,3‑diketo‑L‑gulonate‑binding periplasmic protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTGACGCACCACGCCTATTCCCCGCTTCTGGTGGTGATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCG  >  NC_000913_3_bme_pgi/3746774‑3747063                                                                                                                                                     |                                                                                                                                              tctGACGCACCACGCCTATTCCCCGCTTCTGGTGGGGATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCaaa                                                                                                                                               >  2:242961/1‑149 (MQ=255)  ctGACGCACCACGCCTATTCCCCGCTTCTGGTGGTGATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCTCAAACTGGTTGCTGAAGATCAGCaaaa                                                                                                                                              <  2:39297/149‑1 (MQ=255)             cGCCTATCCCCCGCGTCTGGTGGTGATCAACAAAGCGAAGGTTGATTGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACg                                                                                                                                   <  1:144533/149‑1 (MQ=255)               ccTATTCACCGCTTCTGGTGGTGATCAACAAAGCGAAGTTTGATGGCTTAAGTCAGGAGTTCCAGACAGCGCTAGTTTCATCTGCACAAGAACAGGGTCACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAACATCATCGACGGc                                                                                                                                 >  2:73923/1‑149 (MQ=255)                          cTTCTGGTGGTGATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGc                                                                                                                      >  1:289213/1‑149 (MQ=255)                                 tggtgATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTg                                                                                                               >  1:648939/1‑149 (MQ=255)                                 tggtgATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCAACGGCCTGAAAGAAGCGGGCGTg                                                                                                               >  2:437685/1‑149 (MQ=255)                                    tgATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGCCGGCATGAAAGAAGCGGGCGTCGac                                                                                                            <  2:299647/149‑2 (MQ=255)                                    tgATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGaa                                                                                                            <  1:264041/149‑1 (MQ=255)                                    tgATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGaa                                                                                                            <  1:264385/149‑1 (MQ=255)                                    tgATCAACAAAGCGAAGTTTGATGGCGTAAGTCCGGAAGTCCAGGAGGCGCGAGTTTCATCTGCACAAGAAGCGAGTAACTATCAGCGCAAACTGGTTGCTGAAGAAAAAAAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGaa                                                                                                            <  2:34700/149‑1 (MQ=255)                                     gATCAACAAAGCGAAGTTTGATTGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAg                                                                                                           <  2:766437/149‑1 (MQ=255)                                      aTCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGt                                                                                                          >  2:608897/1‑149 (MQ=255)                                      aTCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATAATCGACGGCATGAAAGAAGAGGGCGTGGAAGt                                                                                                          >  2:568277/1‑149 (MQ=255)                                      aTCAACAAAGCGAAGTGTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGt                                                                                                          >  1:673848/1‑149 (MQ=255)                                        caacaaAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGtca                                                                                                        >  2:563389/1‑149 (MQ=255)                                         aacaaAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGtcat                                                                                                       >  2:626356/1‑149 (MQ=255)                                                    gTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCCAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCg                                                                                            <  1:511520/149‑1 (MQ=255)                                                      ttGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGAc                                                                                          <  1:36155/149‑1 (MQ=255)                                                      ttGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGAc                                                                                          <  1:608897/149‑1 (MQ=255)                                                            gCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCaaa                                                                                    >  2:237549/1‑149 (MQ=255)                                                              ttAAGTCCGGACTTACAGCAGGACCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAATCTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGc                                                                                  <  2:559399/149‑1 (MQ=255)                                                                aaGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCt                                                                                >  1:705553/1‑149 (MQ=255)                                                                   tCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTa                                                                             >  1:441363/1‑149 (MQ=255)                                                                          ttCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGc                                                                      >  1:241444/1‑149 (MQ=255)                                                                            ccAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACAGCAAAGCGTTTAGCGACGCAc                                                                    >  1:572139/1‑149 (MQ=255)                                                                                cagGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTggg                                                                <  1:237549/149‑1 (MQ=255)                                                                                     gcTAGTTTCCTCGGCAAAACAAGCGGTGAACTGTGAGCGCAACCTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATc                                                           <  2:232566/149‑1 (MQ=255)                                                                                      cTAGTGTTACCTGGAGAAGAAGCGGAGAAGTAGCAGCTCAAACTGGTTGCTGAAGATCAGCAAAAAATTATCGACGGCATGAAAGAAGCGGGCGTGTAAGTCATCATCGATCTCGACCGCAAAGCCTTTGGCGACGCACTGGGGAATCa                                                          <  2:43227/149‑1 (MQ=255)                                                                                       tAGTTTCATCCGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCAGACATTCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAg                                                         <  1:756553/149‑1 (MQ=255)                                                                                              aTCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAAAAAGGTTggcg                                                  >  2:118635/1‑149 (MQ=255)                                                                                               tCTGCACAAGAAGCGGGTAACTATCAGCGCAAACGGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGa                                                 <  1:626356/149‑1 (MQ=255)                                                                                                      gagcggCGGATAACTATCATCGCAAACTGGTTGCTGAATATCAGCAAAAAATCATCGACTGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAACGCCCTTAGCGACCCACTGGGGAATCAGGTTCGCGACAtgttt                                          >  2:71537/6‑149 (MQ=255)                                                                                                      aaggagCGGGTAACTATTGGAGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGACGTCATAACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACAtgttt                                          <  2:128219/149‑1 (MQ=255)                                                                                                            cGGGTACCTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTaaa                                    <  1:78086/149‑1 (MQ=255)                                                                                                               gtcaCTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGACATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGAt                                 <  1:35748/146‑1 (MQ=255)                                                                                                               gTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGAACGCAACGCCTTTAGAGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGAt                                 >  1:723812/1‑149 (MQ=255)                                                                                                                tAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGaa                                                                                                            <  1:100301/73‑1 (MQ=255)                                                                                                                tAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGaa                                                                                                            >  2:100301/1‑73 (MQ=255)                                                                                                                   cTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGc                             <  2:289213/149‑1 (MQ=255)                                                                                                                    tgccAGCGTAAACTGGTTGCTGAACATCAGCAAAAAATCATCCACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCTCGACATGTTTGTTAAAGATGTGcc                            <  1:769911/146‑1 (MQ=255)                                                                                                                    tATCCGCCCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCTCCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGcc                            <  2:371866/149‑1 (MQ=255)                                                                                                                         gcgcAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAgg                       >  1:744461/1‑149 (MQ=255)                                                                                                                              aaCTTGTTTCTGGAGATCAGCAAAAAATCATCGACGGCCTGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCt                  <  2:499823/149‑1 (MQ=255)                                                                                                                                 tGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAGGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATCTTTGTTAACGATGTGCCGCAGGGAGCTGAt               >  1:308499/1‑149 (MQ=255)                                                                                                                                 tGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGAt               <  2:441363/149‑1 (MQ=255)                                                                                                                                    ttGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGTCGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATctg            <  2:673848/149‑1 (MQ=255)                                                                                                                                     tGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTcgc                                                   <  1:283651/109‑1 (MQ=255)                                                                                                                                     tGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTcgc                                                   >  2:283651/1‑109 (MQ=255)                                                                                                                                         gAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGAGACGCAATGGGTAATCAAGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGCGCTGATCTGCTGaa       >  1:757751/1‑149 (MQ=255)                                                                                                                                              tCAGAAAAAAATCACCGACGGCATGAAATAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTATCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCg  <  1:437685/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                              TCTGACGCACCACGCCTATTCCCCGCTTCTGGTGGTGATCAACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCAGCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCGACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCG  >  NC_000913_3_bme_pgi/3746774‑3747063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.