New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 4456647	40 (0.900)	4 (0.090)	4/272	5.9	9.4%	coding (862/1374 nt)	mpl	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
	?	NC_000913_3_bme_pgi	= 4456657	39 (0.920)					coding (872/1374 nt)	mpl	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTGGCAA  >  NC_000913_3_bme_pgi/4456510‑4456803                                                                                                                                                    |                                                                                                                                                   gtgAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATCTGCACAATGGCCTGATGGCGATTGAAGCGGcc                                                                                                                                                   >  2:77065/1‑148 (MQ=255)    aGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGGAATGGTCGCTGGTAGGCGACCATAATATGCACAATGGCCTGGTGGCGATTGCGGCGGCTCgg                                                                                                                                                >  1:234513/1‑148 (MQ=255)         ggTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCAtct                                                                                                                                           >  2:174852/1‑147 (MQ=255)           tCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTg                                                                                                                                         <  1:326370/149‑1 (MQ=255)               tGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGgtgt                                                                                                                                     >  2:420174/1‑149 (MQ=255)                   aGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCG‑AAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAgcgc                                                                                                                                >  2:249099/1‑149 (MQ=255)                                  ccaccGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGCTGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCCAATCCCGCt                                                                                                                  >  1:545990/1‑149 (MQ=255)                                    accGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTaa                                                                                                                >  2:547439/1‑149 (MQ=255)                                      cGATGCTTCCGAATGGGAAGTTTTGGTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTGCGCGAACATAATATGCATAATGGCCTGATGGAGATTGCAGCGGCTCGGCATGTTGGTGTAGCGCCGGCAGATGCCGCTAAcg                                                                                                              >  1:698855/1‑149 (MQ=255)                                            ttCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATCATATGCACAATGGACTGATTGCGGTTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGAAGATGGCGCTAACGGGCTgg                                                                                                        >  1:567908/1‑149 (MQ=255)                                            ttCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGTCGATTACAGCGGCTCGCCATGTTGGTGTGGCGCCGGCAGATGCCGCTAAGGCGCTgg                                                                                                        >  2:208663/1‑149 (MQ=255)                                                    gggAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTAGACGGTAGGCGAAAATACTATGCACACTGGTTTGATGGCGATTGTGGCGGTTCGCCCTACCGGTTTCCCGCCGGCACATGCCGCGAACGCGCTGGTGTCGttt                                                                                                >  2:646360/1‑149 (MQ=255)                                                     ggAAGTTTTGCTGGATGGCG‑AAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTttat                                                                                              <  1:249099/149‑1 (MQ=255)                                                          ttttGCTGGATGGCGAAAAAGTGGGCGAAGCGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTTGTTTATTAAt                                                                                          <  1:675733/149‑1 (MQ=255)                                                                 ggATTGCGAAAAAGTGGGCGAAGACAAATGGTCGGTGGTAGGGGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGTCATGTTGGTGTAGCGCCGGCAGATGGCGCTAACGCGCTGGGTTCGTTTATTAATGCtcgtc                                                                                   <  1:88691/149‑1 (MQ=255)                                                                        gAAAATGTGGGCGAAGGGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCt                                                                            <  2:314955/149‑1 (MQ=255)                                                                        gAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCt                                                                            <  1:137486/149‑1 (MQ=255)                                                                        gAAAAAGTGGCCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATACCGCTAACGCGCTTGGTTCGTTTATTAATGCTGGTCGCCGTCt                                                                            >  2:429254/1‑149 (MQ=255)                                                                                     aaGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGgt                                                                                                                                       <  1:312439/77‑1 (MQ=255)                                                                                     aaGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGgt                                                                                                                                       >  2:312439/1‑77 (MQ=255)                                                                                      aGTGAAAGGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCgtggtg                                                              <  2:656540/149‑1 (MQ=255)                                                                                             tGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGaa                                                       <  1:218815/149‑1 (MQ=255)                                                                                              ggTCGCTGTTAAGCGAACATAATACGCACAACACCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGCCACATGCCGCTAACGCGCTCGGTTCGTTTATTAACGCTCCGCGCCGTCTGGAGTTGCGTGGTGAAGCGAAt                                                      <  2:234513/149‑1 (MQ=255)                                                                                                     ggTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCAGGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGGTCGTTTAGTAATGCTCGTCGCCGTCTGGAGTTGCGTTGTGAAGCGAATGGGGtag                                               >  1:367151/1‑147 (MQ=255)                                                                                                      gTAGTCGAACATAATATGGACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTTCGTGGTGAAGCGAATGGCGTCAc                                              <  1:77065/149‑1 (MQ=255)                                                                                                      gTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCAc                                              <  1:420174/149‑1 (MQ=255)                                                                                                        aGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACgg                                            >  2:250363/1‑149 (MQ=255)                                                                                                                    tatGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGGCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATAGGACGAtt                                >  2:756512/1‑149 (MQ=255)                                                                                                                    gacgCACAATGGCCTGATGGCGATTGCGGCGGATCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGAtt                                <  1:174852/146‑1 (MQ=255)                                                                                                                        cacaATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGc                            >  1:33759/1‑149 (MQ=255)                                                                                                                          caATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCg                                                                               >  2:443187/1‑96 (MQ=255)                                                                                                                          caATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCg                                                                               <  1:443187/96‑1 (MQ=255)                                                                                                                          caATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGccc                          <  2:35613/149‑1 (MQ=255)                                                                                                                               gCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACg                                   <  2:365143/135‑1 (MQ=255)                                                                                                                               gCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACg                                   >  1:365143/1‑135 (MQ=255)                                                                                                                                 cgtaTGGCGATTGCAGCGGGTCTCCATGTTGGTGTAGCGCCGGCAGATTCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCAccc                   <  1:641763/146‑1 (MQ=255)                                                                                                                                     tGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGCAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATGACTCGACg               <  2:366179/149‑1 (MQ=255)                                                                                                                                       gCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATTGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGc             >  1:531380/1‑149 (MQ=255)                                                                                                                                              cAGCGGCTCGACATGTTGGTGTAGTGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTg      <  2:461296/149‑1 (MQ=255)                                                                                                                                               aGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTgg     >  2:50840/1‑149 (MQ=255)                                                                                                                                               aGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTgg     >  1:475550/1‑149 (MQ=255)                                                                                                                                                  ggCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTGGCaa  <  1:733498/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATGTTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAAGCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTGGCAA  >  NC_000913_3_bme_pgi/4456510‑4456803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.