New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 407659730 (0.990)4 (0.140) 4/280 3.9 12.3% coding (452/873 nt) yihY BrkB family putative transporter, inner membrane protein
?NC_000913_3_bme_pgi = 4076609 28 (0.950)coding (464/873 nt) yihY BrkB family putative transporter, inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTG  >  NC_000913_3_bme_pgi/4076460‑4076743
                                                                                                                                         |                                                                                                                                                  
ggCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAg                                                                                                                                         >  1:88519/1‑149 (MQ=255)
    tGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGc                                                                                                                                     <  1:462465/149‑1 (MQ=255)
           tGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTg                                                                                                                              >  2:157825/1‑149 (MQ=255)
                gTCACCGGGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGAt                                                                                                                         <  2:480341/149‑1 (MQ=255)
                     gcttttATTGTTGATGTATTCTCTCTATAGCGAGTTGAATCGTATTTGGCGTAGTAAACGAGGGCGAGCATAAATTTACTCGTTCGCCCGGTACTGGATGATTTTAACGCAGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGtt                                                                                                                    <  2:251369/145‑1 (MQ=255)
                     cgcgTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGtt                                                                                                                    >  2:219104/1‑149 (MQ=255)
                      gcgTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAACCGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTc                                                                                                                   >  1:76308/1‑149 (MQ=255)
                                      aCTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCCCTccccg                                                                                                   >  2:46577/1‑147 (MQ=255)
                                            tCGATAGCGCGTTGAATACCATCTGGCCCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTgg                                                                                             <  1:244723/149‑1 (MQ=255)
                                                          aaTACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCaa                                                                               <  1:359159/149‑1 (MQ=255)
                                                              ccATCTGGCGCAGTAAACTGGCGCGATCAAAAATTTACTCGTTCGCCGTTTACTGGATGATTTTAACGCTGGGGCCGCCGCTGTCAGGGGCCAGTCTGGCGATCATTTCCACATCATGCGCCCGGTGCTGGGCGAGCGATCTCAATACt                                                                           <  2:250270/149‑1 (MQ=255)
                                                              ccATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACt                                                                           <  1:219104/149‑1 (MQ=255)
                                                               cATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGCGCGATCTCAATACTg                                                                          >  2:364577/1‑149 (MQ=255)
                                                                  cTGGCGCAGTAAACGAGCGCGACCAAATATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCa                                                                       <  2:279630/149‑1 (MQ=255)
                                                                           tAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACg                                                              <  1:157825/149‑1 (MQ=255)
                                                                               cGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGtgct                                                          <  1:16953/149‑1 (MQ=255)
                                                                                           aaaaTTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTcc                                              <  1:73659/149‑1 (MQ=255)
                                                                                           aaaaTTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTcc                                              <  1:432398/149‑1 (MQ=255)
                                                                                           aaaaTTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTcc                                              <  1:427171/149‑1 (MQ=255)
                                                                                             aaTTTACTCGTTCGCCGTTTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGt                                            <  2:400127/149‑1 (MQ=255)
                                                                                              atctACTCGTGCGCCGTGTACTGGATGATTTTACCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGtt                                           <  1:265688/146‑1 (MQ=255)
                                                                                                    tCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACTTGATGCGTATTTTTCCGTTGGtgtt                                     >  2:413419/1‑149 (MQ=255)
                                                                                                    cggggcGCCGGGTACTGGATGATGTTAACCCGGGGGCCGCTGCTGGCAGGGTCCAGTGGGGGCATCCGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCtgtt                                     <  2:298122/144‑1 (MQ=255)
                                                                                                             gtgtACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAt                            >  1:462966/1‑149 (MQ=255)
                                                                                                                       tgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTTGCGATCAGTTCCTATTTGCTCTGCCTGCGCTGGGCGAGCGAGCTCAATACTCTTATCGCCACCGAGCTGCGTATTTGTCCGTTCCTGTTGTCTGGGAGCTGCTTCttg                  >  2:407206/1‑147 (MQ=255)
                                                                                                                               aCGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCAGCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTa          <  1:110286/149‑1 (MQ=255)
                                                                                                                                    ggggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCTTGCGCTGGGCGAGCGATGTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCa     <  1:495058/149‑1 (MQ=255)
                                                                                                                                    ggggCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCa     <  1:165963/149‑1 (MQ=255)
                                                                                                                                      ggCCGCCGCTGGCAGCGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCAtt   <  1:118144/149‑1 (MQ=255)
                                                                                                                                       gCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTg  >  2:517424/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
GGCGTGCGGGCTGATCGTCACCGCGTTATTGTTGATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTG  >  NC_000913_3_bme_pgi/4076460‑4076743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.