Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,486,574	C→A	A152E (GCA→GAA)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,486,574	0	C	A	96.5%	80.5 / NA	29	A152E (GCA→GAA)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (18/10);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACAC  >  NC_000913_3_bme_pgi/3486430‑3486719                                                                                                                                                 |                                                                                                                                                  gCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCCCGGACTCTGCTGCATCTTGAaaaa                                                                                                                                               >  2:182394/1‑149 (MQ=255)     agtGATTCATGGAAACGCGGACATTCGGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaacaac                                                                                                                                           <  1:304702/147‑1 (MQ=255)      ttGATTCAGTTAAACCCGCACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAAcc                                                                                                                                          <  1:90661/149‑1 (MQ=255)          ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGAc                                                                                                                                      >  2:280204/1‑149 (MQ=255)                       ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGCGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAACCTGGAAAAACAACCCGACGCCATGACTCAcc                                                                                                                         >  1:127647/1‑149 (MQ=255)                                      tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCcaa                                                                                                          >  1:335606/1‑149 (MQ=255)                                       ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGACTGCAAAt                                                                                                         >  1:273805/1‑149 (MQ=255)                                          tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGAAGATCaa                                                                                                      >  1:514995/1‑149 (MQ=255)                                                gaggtGGCGGGTCGACTGCAAGTCACTTCAGACAAAGCGGGCAACCTGGCCTTCCTCGACCTCACGGGCCGCCTCGCACAGTCCCTGCTCAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAc                                                                                                <  2:342708/145‑1 (MQ=255)                                                    tGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTCCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGt                                                                                            <  2:476531/149‑1 (MQ=255)                                                      gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                          >  1:389582/1‑149 (MQ=255)                                                      gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                          >  1:478080/1‑149 (MQ=255)                                                      gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                          >  2:147600/1‑149 (MQ=255)                                                        gcgTCGTCTGCGCGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCTCATTGCACAGACTCTGCTGAAGCTGAAAAAACAACCAGACGCTATGCCTCACCCGGCCGGGATGCAAATCAACATATCCCGTCAgt                                                                                        >  2:35498/1‑148 (MQ=255)                                                              tcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                  <  1:305220/149‑1 (MQ=255)                                                              tcTGCAAGTCAAGTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGGGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGTCGGTATGCAAATCAACATTACCCGTCAGGAAATTg                                                                                  <  2:454718/149‑1 (MQ=255)                                                                tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCAGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATAACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                >  2:528255/1‑149 (MQ=255)                                                                   aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGTGTCACCCATTCGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                             >  1:524978/1‑149 (MQ=255)                                                                       cACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAAGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTg                                                                         <  1:437913/149‑1 (MQ=255)                                                                        aCTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATGTGGCAAAACAACCAGACGCTATAAGTCACCCGGTCGGTATGCAAATCAAAATTACCCGTCAGGGAATTGGGCCGATTGt                                                                        >  1:149777/1‑149 (MQ=255)                                                                             agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCCAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                   <  2:128450/149‑1 (MQ=255)                                                                             agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                   <  1:280204/149‑1 (MQ=255)                                                                                        gCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAACCAACCAGACGCTATGACTCACCCGGCCGGTATGCCAATCAAAATTACCCGTCAGGAAATTGCTCAGATTATCGCCTGTTTTCGTGaa                                                        >  1:262195/1‑149 (MQ=255)                                                                                                         gACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                       >  2:106834/1‑149 (MQ=255)                                                                                                             tGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAg                                   >  2:36547/1‑149 (MQ=255)                                                                                                                 gggACGGATTGCACAGTGTCTGCCGTATGGTGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGc                               <  2:268583/149‑1 (MQ=255)                                                                                                                                     tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGACAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGCTCATAACCTGAta           >  2:445584/1‑148 (MQ=255)                                                                                                                                        tgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc        >  1:223228/1‑149 (MQ=255)                                                                                                                                              tGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAGATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTGTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCacac  >  2:340797/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACAC  >  NC_000913_3_bme_pgi/3486430‑3486719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑