New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 4280509 =30 (0.840)5 (0.150) 5/268 4.1 15.8% coding (529/1650 nt) yjcE putative cation/proton antiporter
?NC_000913_3_bme_pgi 4280529 = 25 (0.730)coding (549/1650 nt) yjcE putative cation/proton antiporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCT  >  NC_000913_3_bme_pgi/4280364‑4280654
                                                                                                                                                 |                                                                                                                                                 
tgtgGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCg                                                                                                                                                >  1:388969/1‑149 (MQ=255)
tgtgGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCg                                                                                                                                                >  1:389007/1‑149 (MQ=255)
          tcCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGc                                                                                                                                      >  1:77326/1‑149 (MQ=255)
            cGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTACAGTTTGCCGTAGCCGTGGCGCTGGGGACGATGATCTTCACCGTCGGCGGTGCGa                                                                                                                                    >  2:173867/1‑149 (MQ=255)
            agggATTGAAGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGa                                                                                                                                    >  2:6584/2‑149 (MQ=255)
                    ttgGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGaa                                                                                                                            <  2:332177/149‑1 (MQ=255)
                        cGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTa                                                                                                                        <  2:176211/149‑1 (MQ=255)
                              gcgcATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATATTCACCGTCGGCGGTGCGACGGTCGAATTTAGGaagg                                                                                                                  >  2:312881/1‑147 (MQ=255)
                               cgcATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGt                                                                                                                 <  1:237784/149‑1 (MQ=255)
                                      cGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCAtt                                                                                                          >  1:79779/1‑149 (MQ=255)
                                      cGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGAGGAACTTCACTGTCGTTGGTGCGACGGTCGAATTTATGAAAGTAGCCAtt                                                                                                          >  2:611128/1‑149 (MQ=255)
                                                    ggCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGc                                                                                            >  1:208571/1‑149 (MQ=255)
                                                                ggCCAAGCGTTGATGAACAACGCCCCCGGCCTGGACTCCTTGAAGTTTGCCGGAGCAGTGGCGATGGGGACGAAGAGCCTCACCCGCGTCGGTGCAACGCTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCCGTTTTgtggt                                                                                <  2:273608/149‑1 (MQ=255)
                                                                       cGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTgtggtgggatga                                                                         >  2:55702/1‑143 (MQ=255)
                                                                          tgatgaATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAgctggctg                                                                      >  1:20190/1‑149 (MQ=255)
                                                                             tgaatgaCGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTAc                                                                   <  1:285393/149‑1 (MQ=255)
                                                                                 tgaCGGTTCCGGGTTGGGGTCGTTGAAGTGTGCGGTAGCAGTGGCGATGGGGATGATGATCTTGACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTTGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGtc                                                               <  2:374361/149‑1 (MQ=255)
                                                                                            gTCTGGTGTCGTTGACGTTTGCCGTAGCAGTGGCGATGGGGAAGCTGATATTCATCTTCTGCTTATCGACGGGCGAATTCATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGa                                                    <  2:314573/149‑1 (MQ=255)
                                                                                                 gtgtCGTCGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGTAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTATGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCt                                               >  1:589000/1‑149 (MQ=255)
                                                                                                                gCCGTAGCAGTGGCGAGGGGGACGCGGATCTTCGCCGTGGGCGGTGTGACGGTCGAATTTATGAAAGTGGCCATTGGCGGTATTCTCGCCGGGTTTGTGGTGGGCGGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCgg                                <  2:399286/149‑1 (MQ=255)
                                                                                                                 ccGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGGGCGACGGTCGAATGTATGAATGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGt                               <  2:233073/149‑1 (MQ=255)
                                                                                                                   gTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGtga                             <  1:6584/149‑1 (MQ=255)
                                                                                                                               aTGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGAc                 >  1:400317/1‑149 (MQ=255)
                                                                                                                                 ggggACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGc               <  1:59001/149‑1 (MQ=255)
                                                                                                                                    gACGATGATCTTCACCGTCGGTGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGa            <  1:81081/149‑1 (MQ=255)
                                                                                                                                      cGATTATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTTGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATc          <  1:70733/149‑1 (MQ=255)
                                                                                                                                       gatgatCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCg         <  2:79779/149‑1 (MQ=255)
                                                                                                                                              ttCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGtc                                                               >  2:605794/1‑88 (MQ=255)
                                                                                                                                              ttCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGtc                                                               <  1:605794/88‑1 (MQ=255)
                                                                                                                                              ttCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGACCGTCctgct  >  2:591601/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCT  >  NC_000913_3_bme_pgi/4280364‑4280654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.