| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,182,820 | C→T | H526Y (CAC→TAC) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,182,820 | 0 | C | T | 97.9% | 146.8 / NA | 47 | H526Y (CAC→TAC) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (25/21); minor base A (1/0); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAT > NC_000913_3_bme_pgi/4182679‑4182959 | ctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACACCCCGCTGTCTAAGATTACGTACAAAcg > 2:183682/1‑149 (MQ=255) tctGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGACCAAACGTCGTa > 1:7551/1‑149 (MQ=255) ggCGATCTGGATACGCTGATGCCACAGTTTCTGGTCACGGCCCAGCCGATTTCCG‑CAGCAGTGTAAGACATCTTCGGTGCAAGCCAGCGGGCTGAGTTTGTGTACCAGAACAACCCGCTGTCTGAGTTTACGTACAAACGTCGTAtatc > 2:343060/1‑149 (MQ=255) gATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGc > 1:612303/1‑149 (MQ=255) gATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGAATCTCCGc > 1:329435/1‑149 (MQ=255) tCTGGATACCCTGAGGCCACAGGAGATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCAc > 1:552699/1‑149 (MQ=255) tACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGcc > 2:89003/1‑149 (MQ=255) ccTGATGCCACAGGATATGATCAACGCCAGGAC‑ATTTCCGCCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCCCACTCGGCC‑CAg > 1:474976/1‑149 (MQ=255) cTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGGGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAgg > 2:343130/1‑149 (MQ=255) cTGATGCCACAGGATATGATCAACGCCAAGCAAATTTCCT‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTTTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGACATTACGTACAAACGTCGTATCTACGCACTCGGCC‑CAgg > 2:300856/1‑149 (MQ=255) gCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTc < 1:89003/149‑1 (MQ=255) cacaGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTg > 2:308527/1‑149 (MQ=255) cacaGGATATGATCAACGCCAAGCCGAGTTCCG‑CAGCCGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTCTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGCCC‑CAGGCgcgctg > 2:329716/1‑144 (MQ=255) tatGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTg > 1:130582/1‑149 (MQ=255) gATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCTCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAg < 1:506188/149‑1 (MQ=255) ttCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTc < 1:29233/149‑1 (MQ=255) ttCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTc < 2:130582/149‑1 (MQ=255) ccG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATCACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCga < 1:89306/149‑1 (MQ=255) g‑cagcaGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGGTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCgaga < 1:567691/149‑1 (MQ=255) gAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAAAGCGCAGGCCGCGAAGTTCGAGCCGTACAcc > 2:265214/1‑149 (MQ=255) aaaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAccc > 1:192271/1‑149 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACt > 1:509517/1‑149 (MQ=255) ttcttgGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGCACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCa < 1:59280/149‑1 (MQ=255) ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGaa > 1:27844/1‑126 (MQ=255) ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGaa < 2:27844/126‑1 (MQ=255) cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTCCACCCGACTCACt > 2:449342/1‑149 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTa < 1:324830/149‑1 (MQ=255) ccagccagCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTcgc < 1:5763/149‑1 (MQ=255) tagcgagcTGTCTGAGTTTATGGACCAGAACAACCCGCTTTCTGAGATTACGTATAAACGTCGTATCTCCGCACTCGGCC‑CTGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTcgcg < 2:570029/148‑1 (MQ=255) agccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt > 1:323147/1‑149 (MQ=255) gcTGTCTCACTTTACGGCCCAGAACTACCCGTTCTCTGTGATTTCGTACCCCCGTCGTATCTCCGC‑CCCGGCCGGAGGCAGTCTTGGATGGCGACTTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTc < 1:627020/149‑1 (MQ=255) gTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCaa < 2:323147/149‑1 (MQ=255) ctcAGTTTATGGAACAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGCGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc < 1:183682/149‑1 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGCCTCACTACGGTCGCGTATGTCCACTCGAAAccccggg > 1:12239/1‑146 (MQ=255) aCCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa > 1:559194/1‑149 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTAGCGTATGTCCAATCGAACCTCCTTAAg > 2:67437/1‑149 (MQ=255) aGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt < 1:253010/149‑1 (MQ=255) gAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTGCCGTATGTCCAATCGAAACCCCTGAAGGTc > 1:250295/1‑149 (MQ=255) gAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGGAGGTc > 2:385455/1‑149 (MQ=255) accaacCCGCTGTGTGGGATTACGTACAAACGTCGGATGCCCGCACTCGGCC‑AAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGAGTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc < 2:107314/147‑1 (MQ=255) acaacCCGCTGGCTGAGATTACGTACACACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg < 2:393161/149‑1 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGAc > 2:318534/1‑101 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGAc < 1:318534/101‑1 (MQ=255) cGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc > 2:350554/1‑149 (MQ=255) tGTCTGAGATTACGTACAAACGTCGTATCCCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAATGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt < 1:343060/149‑1 (MQ=255) gagaTTACGTACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt < 1:295273/149‑1 (MQ=255) gagaTTACGTACAAACGTCGTAACTCCGCCCTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTTCCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt < 2:164693/149‑1 (MQ=255) | CTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCG‑CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCC‑CAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAT > NC_000913_3_bme_pgi/4182679‑4182959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |