Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,8000AG22.5% 77.3 / 16.9 42E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (15/16);  new base G (3/6);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTAT  >  NC_000913_3_bme_pgi/526658‑526944
                                                                                                                                              |                                                                                                                                                 
gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATa‑tgat                                                                                                                                            <  2:253215/149‑1 (MQ=255)
    gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAACAGCTCATCTTTATAAATAAGACCACCGGGGACTGCCGCTGGCGCTTGTCAGCGAAGACGGCAGTACGGCGTGGAGCGCGGCAtg‑tgctatat                                                                                                                                        >  2:148696/1‑143 (MQ=14)
    gCTGACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAAt                                                                                                                                        <  1:255320/149‑1 (MQ=11)
      tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGATGAAGATTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATgg                                                                                                                                      <  1:368673/149‑1 (MQ=11)
      tGACGGCGGAGCAACTGGCCAGACACGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTGTATCACTGCGACCACCACGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATACGGGGTGGCGCGGGGAGTA‑TGATGAATgg                                                                                                                                      <  1:477749/149‑1 (MQ=11)
      cgACGGTGGAGGAACGGGCCTGACAGGGGGGGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATgg                                                                                                                                      <  2:280531/148‑1 (MQ=17)
       gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATggg                                                                                                                                     <  1:180740/149‑1 (MQ=255)
       gACGGCGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAGAGCTCATCTTTATCAACGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGACAATACGGCGTGGCGCGGGGAGTA‑TGATGAATggg                                                                                                                                     <  2:69540/149‑1 (MQ=11)
                gaaaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATgg                                                                                                                                      >  1:304010/3‑139 (MQ=255)
                gaaaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATgg                                                                                                                                      <  2:304010/137‑1 (MQ=255)
                  aaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCtt                                                                                                                          >  2:460373/1‑149 (MQ=255)
                   aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGTGGCAACCAGCTTa                                                                                                                         >  1:219290/1‑149 (MQ=255)
                    cTGGCCAGACAGGTGGAGACGGAATACACACCGGCGCGAATAGCTCATTATTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTaa                                                                                                                        <  2:55789/149‑1 (MQ=255)
                       gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATGGGGCAACCAGCTTAATga                                                                                                                     >  1:228745/1‑149 (MQ=255)
                         cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATga                                                                                                                     >  2:3762/1‑147 (MQ=255)
                         cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATga                                                                                                                     <  1:3762/147‑1 (MQ=255)
                          aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                  >  1:362007/1‑149 (MQ=255)
                          aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                  >  2:415063/1‑149 (MQ=255)
                           gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGAATCCCCGCGGCCCCTCTCAGAGACGGAGGCAATCCGTCCTGACGCG‑GGAGGACTGCTGAATGGGGCAACCAGCGTGGTgaggga                                                                                                                 >  2:85540/1‑147 (MQ=2)
                              aGGTGTAGCCAGAATACATACTCGCGCGAAAAGCTAATCTATATAACTGCGAACAGCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGTCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                              <  2:192789/149‑1 (MQ=17)
                                       cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                     <  1:280965/149‑1 (MQ=255)
                                         gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGCA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcaacacca                                                                                                   >  2:432369/1‑146 (MQ=11)
                                            tACACACCGGCGCGAAAAGCTCATCTTGATCACGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgca                                                                                                <  2:353834/149‑7 (MQ=1)
                                             gcacacCGGCGCGAAAACCTTATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                               <  2:333792/148‑1 (MQ=255)
                                              cacccCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                              <  2:142039/149‑1 (MQ=255)
                                              cacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                              <  1:415063/149‑1 (MQ=255)
                                               accccGGCGGGAGAAGCTCATCTTTATCATTGCGACCACCGGGGACTGGCGCTGGCGGTTATCAGCGAAGACGGCAATACGTCGTGGAGCTCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                             <  2:305426/146‑1 (MQ=17)
                                                 acCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTTTATCGGc                                                                                           >  1:17764/1‑149 (MQ=255)
                                                                tCATCTTTACCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                            <  1:121645/149‑1 (MQ=255)
                                                                  aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                          >  1:151650/1‑149 (MQ=255)
                                                                          cACTGCGACCACCGGGGACTCCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGCGGACTA‑TGATGAATCGGGCAACCAGCTTAATGAGGAGCACCCGCATCATGTGTATCCGCCTGACCGCCTCCCCGGccagcatca                                                                  >  2:315856/1‑149 (MQ=14)
                                                                                   caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                         >  1:218964/1‑149 (MQ=255)
                                                                                        gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                                    <  1:288743/149‑1 (MQ=255)
                                                                                          gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                  <  1:460373/149‑1 (MQ=255)
                                                                                             tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGACAA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                               >  1:498333/1‑149 (MQ=255)
                                                                                               ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                             >  1:351908/1‑149 (MQ=255)
                                                                                                     gcgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                       >  2:340924/1‑149 (MQ=255)
                                                                                                            tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                                >  2:394546/1‑149 (MQ=255)
                                                                                                              aGCGAAGACGGCAATACGGCGTGGAGCGCTGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                              <  2:219290/149‑1 (MQ=255)
                                                                                                                         cAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                   <  2:218964/149‑1 (MQ=255)
                                                                                                                                       gcgcGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCgg     >  2:311264/1‑149 (MQ=255)
                                                                                                                                          ctgAATA‑TGATGACTGGGGCAAAAAGCTTGATAAGGAGGACCCGCATCTTGTGTATCAGCGGATCCTTCTGCAAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGtat  >  2:369665/3‑149 (MQ=17)
                                                                                                                                              |                                                                                                                                                 
GCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTAT  >  NC_000913_3_bme_pgi/526658‑526944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑