New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	781318 =	51 (1.290)	5 (0.130)	5/270	4.8	9.0%	intergenic (+96/‑51)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
	?	NC_000913_3_bme_pgi	781335 =	53 (1.420)					intergenic (+113/‑34)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAG  >  NC_000913_3_bme_pgi/781192‑781477                                                                                                                                                  |                                                                                                                                               gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                                                                                                           <  1:442941/149‑1 (MQ=255)  tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCCCCAATCTTAAAGATTGGCCCCGAg                                                                                                                                          >  1:572614/1‑149 (MQ=255)        gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCCATCTTAAAGATTGGCCCCGAGaaaaaa                                                                                                                                    >  1:605465/1‑149 (MQ=255)        gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaaa                                                                                                                                    <  1:98371/149‑1 (MQ=255)               tCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCTATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                                                                                             <  2:547104/149‑1 (MQ=255)               tCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                                                                                             <  2:670111/149‑1 (MQ=255)                ccTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACTCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAg                                                                                                                            <  2:288605/149‑1 (MQ=14)                  tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                                                                                          >  1:182125/1‑149 (MQ=255)                          ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCg                                                                                                                  >  1:22754/1‑149 (MQ=255)                            accaGTTTTAACATCAAACTCAGATGTTAAGCGTGACGGATAACGTTGCGCCAGCAACGGCCCGTCGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCACCCAGTAAAAAACTTACCGGTAACACACGTa                                                                                                                >  2:117724/1‑149 (MQ=21)                            accaGTTTGAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa                                                                                                                <  2:306955/149‑1 (MQ=255)                             caggTTTTAGGATCAAACTCGGATGGTAAGCGTGAAGGATAACGTTGCGTCATCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                                                                                                               <  2:157613/146‑1 (MQ=25)                                gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                                                                                                            >  2:597973/1‑149 (MQ=255)                                gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                                                                                                            <  1:443437/149‑1 (MQ=255)                                 ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACGCACCAAGCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                                                                                                           >  1:306503/1‑149 (MQ=255)                                  tttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                                                                                          >  2:337157/1‑149 (MQ=255)                                    tAACAGCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAACTCTTTCAGGTAACACCCGTATGGGTCGt                                                                                                        <  1:361406/149‑1 (MQ=255)                                       cATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCAATCGACCGGAAATAAGTGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg                                                                                                     >  2:493055/1‑149 (MQ=21)                                         tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt                                                                                                   <  1:197145/149‑1 (MQ=255)                                         tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt                                                                                                   >  1:169246/1‑149 (MQ=255)                                           aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAGTCTTAAAGGTTGGCGCAGCGTAGAGAACTTTCCGGTAGTACCCGGATGGTTCGtttacaac                                                                                                 >  2:478628/1‑143 (MQ=17)                                                cAGATGTTAAGCGGGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                                                                                            <  2:530254/149‑1 (MQ=255)                                                  gATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                                                                                          <  1:321094/149‑1 (MQ=255)                                                       tAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                     <  2:668627/149‑1 (MQ=255)                                                          gCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                  <  1:522431/149‑1 (MQ=255)                                                               aaGTATAACGGTGCGCGGGCACCGGCCCGGAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCATCACTCTTAAAGATTGGCCCCGAGGAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                             <  2:84457/149‑1 (MQ=37)                                                                  gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTAATGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTa                                                                          <  1:482241/149‑1 (MQ=255)                                                                   aTAACTTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                         <  1:387685/149‑1 (MQ=255)                                                                   aTAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                         <  1:447514/149‑1 (MQ=255)                                                                      aCGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGGGCAGTTGACTTTTAATCa                                                                      >  1:247250/1‑149 (MQ=255)                                                                               cAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc                                                             >  1:104991/1‑149 (MQ=255)                                                                                          cGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATc                                                  <  1:106561/149‑1 (MQ=255)                                                                                           gaaGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                                                                                                             >  2:33130/3‑59 (MQ=11)                                                                                           gaaGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                                                                                                             <  1:33130/57‑1 (MQ=11)                                                                                               ggCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCa                                             <  2:169246/149‑1 (MQ=255)                                                                                                        aag‑cgaGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccac                                     <  1:337157/149‑1 (MQ=255)                                                                                                                     cTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAggt                         >  1:301381/1‑149 (MQ=255)                                                                                                                       gCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAggtgg                       <  1:146547/149‑1 (MQ=255)                                                                                                                           gACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCTCTACCCACCAATTTAAAGGTGGTTAc                   >  1:5198/1‑149 (MQ=255)                                                                                                                           gACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTaaa                            >  2:455653/1‑140 (MQ=255)                                                                                                                           gACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTaaa                            <  1:455653/140‑1 (MQ=255)                                                                                                                           gACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGATCGCAGGTTCGAATCCTGCACGACCCACCAATTTAACGCTGGTTAc                   >  2:417704/1‑149 (MQ=255)                                                                                                                            cggggccaATCTTTAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACACCCCAATTTAAAGGTGGTTACt                  >  2:188619/6‑149 (MQ=25)                                                                                                                             cccACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                        <  2:9207/94‑1 (MQ=255)                                                                                                                             cccACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                        >  1:9207/1‑94 (MQ=255)                                                                                                                                gccaATCTTTAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAg                           >  1:78150/2‑136 (MQ=255)                                                                                                                                gccaATCTTTAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAg                           <  2:78150/135‑1 (MQ=255)                                                                                                                                acgaaTATTAAAGATTGGACCCGAGTAAAAATCTTCGAGATAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTa              <  2:385347/146‑1 (MQ=21)                                                                                                                                accaATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTCCTgtca              >  2:167232/1‑146 (MQ=255)                                                                                                                                accaATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTgcaa              >  2:167184/1‑146 (MQ=255)                                                                                                                                 ccaATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTag             <  2:306503/149‑1 (MQ=255)                                                                                                                                  caATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCAACCAATTTAAAGGTGGTTACTGGTaga            >  1:323769/1‑149 (MQ=255)                                                                                                                                   aaTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTagag           >  2:577603/1‑149 (MQ=255)                                                                                                                                      ctaaaaGATGCGCCCCGAATAAAAATCTTACACGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAc        <  1:197511/146‑1 (MQ=21)                                                                                                                                       ttAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACg       <  2:301381/149‑1 (MQ=255)                                                                                                                                         aaaGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTACAGGTGGTTACTGGTAGAGAACGTg     <  1:597973/149‑1 (MQ=255)                                                                                                                                            gATTGGCCCCGGTTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAg  <  2:182125/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               GTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAG  >  NC_000913_3_bme_pgi/781192‑781477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.