New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	2612816 =	57 (1.330)	6 (0.150)	6/272	4.8	9.7%	coding (917/2013 nt)	hyfR	hydrogenase‑4 transcriptional activator
	?	NC_000913_3_bme_pgi	2612847 =	57 (1.390)					coding (948/2013 nt)	hyfR	hydrogenase‑4 transcriptional activator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAAT  >  NC_000913_3_bme_pgi/2612672‑2612960                                                                                                                                                 |                                                                                                                                                 tACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATCCCTCTTCCACTCTCTTTAGTGAGTAAAACTGCCAGCTACTACAACACATAGCCGatcgcatc                                                                                                                                              <  2:274750/149‑1 (MQ=255)  aCTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGatcgcatcg                                                                                                                                             >  1:229006/1‑149 (MQ=255)    ttGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCt                                                                                                                                           >  1:506454/1‑149 (MQ=255)        aTCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTg                                                                                                                                       >  1:184955/1‑149 (MQ=255)         tCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCGGCTACTACAAAACATAGCCGATCGCATCGCTATTGc                                                                                                                                      >  1:464319/1‑149 (MQ=255)         tCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACCTGGCCGATCGCATCGCTATTGc                                                                                                                                      >  2:722002/1‑149 (MQ=255)          cTAACGGCTGCGAATCTGCCCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTAATACAACACATAGCCGATCGCATCGCTATTGcc                                                                                                                                     <  1:556620/149‑1 (MQ=255)                         cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGAt                                                                                                                      <  1:463035/149‑1 (MQ=255)                           gcgcTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGc                                                                                                                    >  1:21089/1‑149 (MQ=255)                              cTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTg                                                                                                                 <  1:257579/149‑1 (MQ=255)                                    ataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTAATGGCGCATACCTCTTCCACGCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGACGATGCCTGGCGTAg                                                                                                           >  1:482140/1‑149 (MQ=255)                                    ataCCACTTACCTGTGGCAACCCTACACTGGCTGCATTGTTGTTTGTTTTTTACTCATCCACCCTCCTCCGTGATCAAAACTGCCTGCTACTCCATCTCCTAGCCCATCGCACCGCTATTGCCGTTGGCAATGCCTATGCCTGGCGTAg                                                                                                           <  2:214779/149‑1 (MQ=255)                                     tatcGCTTACCTTTGCGAACCATACACCCGGTGCAGTGTTTCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGTCAATGCCGATGCCTGGCGTAGc                                                                                                          <  2:290956/146‑1 (MQ=255)                                          cTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAc                                                                                                     <  2:110121/149‑1 (MQ=255)                                           ttAGCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAcc                                                                                                    <  2:666994/149‑1 (MQ=255)                                           ttACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTCCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAcc                                                                                                    <  1:212986/149‑1 (MQ=255)                                               cTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                >  1:577307/1‑149 (MQ=255)                                               cTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCGCGAATATGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                <  1:730906/149‑1 (MQ=255)                                                        ccATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACCCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaaa                                                                                       <  1:43407/149‑1 (MQ=255)                                                                cGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgcag                                                                               >  2:507129/1‑149 (MQ=255)                                                                  ggTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCt                                                                                                                  >  1:236337/1‑112 (MQ=255)                                                                  ggTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCt                                                                                                                  <  2:236337/112‑1 (MQ=255)                                                                       aTTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaa                                                                        >  1:215741/1‑149 (MQ=255)                                                                       aTTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaa                                                                        >  1:628603/1‑149 (MQ=255)                                                                          gttgCTGGCGCATCCCTCTTCCACCCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAacc                                                                     <  1:293569/149‑1 (MQ=255)                                                                            ggCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAaccac                                                                   <  2:506454/148‑1 (MQ=255)                                                                              cTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTAGAACACATAGCCGATCGAATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAAcaacca                                                                 >  1:312420/1‑149 (MQ=255)                                                                                   gcATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGTCTGGCGTAGCATGACCGATTTGCAGGAAAGTGTGCAGCAAGAAAACCACCAGCTTa                                                            <  2:122226/149‑1 (MQ=255)                                                                                     aTACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGc                                                          <  2:215741/149‑1 (MQ=255)                                                                                         ctctTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGagc                                                      >  2:477681/1‑149 (MQ=255)                                                                                               cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAaccac                                                                   >  1:648263/1‑130 (MQ=255)                                                                                               cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAaccac                                                                   <  2:648263/130‑1 (MQ=255)                                                                                                 ctctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCtt                                              <  1:97996/149‑1 (MQ=255)                                                                                                 ctctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCtt                                              >  2:50806/1‑149 (MQ=255)                                                                                                 ctctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCAGATGACTGGCGTAGCCTGCCCGCTTTGCAGGAAAGTTTGCAGCAAGAAATCCACCAGCTTAGCGAGCAGCTCCtt                                              <  2:443436/149‑1 (MQ=255)                                                                                                 cgctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTACTGCCGTTCGAAATGCCGATGCCTGGCCAAGCACGAACGCTTTCCAGCAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCtt                                              <  2:443348/147‑1 (MQ=255)                                                                                                  tctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCttt                                             <  1:50806/149‑1 (MQ=255)                                                                                                  tctctGTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCttt                                             <  2:59625/149‑1 (MQ=255)                                                                                                   ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTc                                            <  2:577307/149‑1 (MQ=255)                                                                                                   ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTc                                            <  2:628228/149‑1 (MQ=255)                                                                                                      tttAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCCCCAGCATAGCGGTCGGCCCCTTTCGaa                                         >  1:358371/1‑149 (MQ=255)                                                                                                         aGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCt                                      <  1:296069/149‑1 (MQ=255)                                                                                                           tGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTgg                                    <  1:250675/149‑1 (MQ=255)                                                                                                            gAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTggg                                   >  2:372425/1‑149 (MQ=255)                                                                                                             aGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATACCGCTGCCTGGCGTAGCATGCCCGATTTGCAGGCAAGTTTGCAGCAAAAAAACCCCCAGCTCCGCGAACAGGCACTTTCGGACATGGGc                                  >  2:398202/1‑149 (MQ=255)                                                                                                                  aaCTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCgg                             <  2:229006/149‑1 (MQ=255)                                                                                                                     tGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGa                          >  2:566062/1‑149 (MQ=255)                                                                                                                           ctactaCAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATtat                    <  1:133651/149‑1 (MQ=255)                                                                                                                                 caacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa              >  1:232594/1‑149 (MQ=255)                                                                                                                                  aacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCaa             >  1:312181/1‑149 (MQ=255)                                                                                                                                   acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCaaa            <  2:312181/149‑1 (MQ=255)                                                                                                                                       aTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCa        <  1:477681/149‑1 (MQ=255)                                                                                                                                          gCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc     >  1:67894/1‑149 (MQ=255)                                                                                                                                           ccGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCa    >  2:72473/1‑149 (MQ=255)                                                                                                                                           ccGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCCCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCa    >  2:11469/1‑149 (MQ=255)                                                                                                                                            ctatcgcatcgcTATTTCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCaa   <  2:312420/147‑1 (MQ=255)                                                                                                                                             gATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAAt  >  1:10282/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAAT  >  NC_000913_3_bme_pgi/2612672‑2612960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.