New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 2662483	49 (1.090)	8 (0.270) +CTACAGTGAACAGAACCGTAGGTCGGATAAGGCGTTCACGCCGCATCCG	4/188	4.3	20.0%	noncoding (175/266 nt)	REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences	REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences
	?	NC_000913_3_bme_pgi	= 2662583	NA (NA)					coding (741/741 nt)	trmJ	tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCA  >  NC_000913_3_bme_pgi/2662340‑2662626                                                                                                                                                 |                                                                                                                                                tGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACgg                                                                                                                                            <  2:82176/149‑1 (MQ=35)    ggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTg                                                                                                                                         >  1:91701/1‑149 (MQ=32)       tgtGTAGACTGTAAGCCTGATAAGACGCATTACGCGTGGCAGCAGGCAACTGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCg                                                                                                                                      <  2:38148/147‑1 (MQ=11)          gTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                       >  2:251700/1‑145 (MQ=32)          gTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                       <  1:251700/145‑1 (MQ=32)                tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑ATGCGCATTACGCGTCGCATGAGGCAACGGCTGTCGGATggggcg                                                                                                                             >  1:657704/1‑149 (MQ=255)                 gTAGGCCTGATTAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGTTGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt                                                                                                                            <  1:138106/149‑1 (MQ=255)                     gCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGAGA‑AGACGCATTACGCGTCGCATCAGGCAAGGGCTGTCGCACGCCGCGtacac                                                                                                                        >  1:430879/1‑145 (MQ=11)                        tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGTTGCGGCGGGAACgtc                                                                                                                     >  1:642559/1‑147 (MQ=255)                         gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCt                                                                                                                    <  1:634196/149‑1 (MQ=255)                          aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCCCTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTGGGATGCAGCGTGTACGCCtt                                                                                                                   >  1:505987/1‑149 (MQ=255)                          aTAAGACGCATTACGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCtt                                                                                                                   <  1:171915/149‑1 (MQ=255)                           tAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGACCGCCTTa                                                                                                                  >  2:67199/1‑149 (MQ=255)                            aaGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTAt                                                                                                                 <  1:132939/149‑1 (MQ=255)                                         cgTCGCATCAGGCACCGGTTGTCGGATGCGGCGCGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTTATA‑AGTCGCAGTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGtt                                                                                                    <  2:755024/149‑1 (MQ=17)                                                tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCa                                                                                             <  2:455744/149‑1 (MQ=255)                                                gtaGGCACCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCa                                                                                             <  2:146537/147‑1 (MQ=255)                                                          gCTGTCGGGTGCGGCGTGAACGCCTTATCCGTCCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCt                                                                                   <  1:695318/149‑1 (MQ=255)                                                             gTCGGATGCGGCGTGAACGTCTTATCCGTCCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCGGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGAt                                                                                <  1:336163/149‑1 (MQ=255)                                                                ggATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATAACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAg                                                                             <  2:630847/149‑1 (MQ=255)                                                                       gcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCAGTACGCGTCGCATCAGGCAACGGCTGTCGGCTGCGGCGTGAACGCCTTATCCGACGTACGGTTCTGTTCACTGTAGGCCTGATAAgtctcagt                                                                      >  1:424092/1‑142 (MQ=14)                                                                            aaCGCCTTATCCGACCTATGGTTCTGTGCACTGTAGGCC‑AATATTGAGTCATTACGAGTAGGTCGTGATTACGTCTGTCGGATGGCGCGTGAACGCCTTATCCGACCTACGGTTGTGGTCACGTTGGCCCCGGTAAGACGCAtttacgc                                                                 >  2:366852/1‑144 (MQ=2)                                                                            aaCGCCTTATCCCACCTACGGTTCTGTTCACTGT‑GGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGt                                                                <  1:435800/149‑1 (MQ=255)                                                                              cGCCTTATCCGCCCCACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGACGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTc                                                               <  1:624391/149‑1 (MQ=255)                                                                               gCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTAACCGACCTACGGTTCTGTTACCTGTACGCCAGATAACACGCATTAcccgcac                                                              >  2:443421/1‑146 (MQ=14)                                                                                tcTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCTTTAACGCCTTATCCGACCTCCGGTTCTGTTCACTGTAGGCCTGATAATACGCATTTCGCGTCGc                                                             >  2:43024/2‑149 (MQ=14)                                                                                 cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCa                                                            >  2:434496/1‑149 (MQ=255)                                                                                           cTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGAAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGGATAAACCGGCGCCTCAGGCAACg                                                  >  1:108854/1‑149 (MQ=11)                                                                                              cGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTCCGTGTCAGAGCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCt                                               <  2:478275/149‑1 (MQ=11)                                                                                              cGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCt                                               <  1:359861/149‑1 (MQ=32)                                                                                                     gTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTc                                        >  1:509143/1‑149 (MQ=35)                                                                                                      ttCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGGTGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCg                                       <  1:654269/149‑1 (MQ=25)                                                                                                        cACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGc                                     <  2:414372/149‑1 (MQ=35)                                                                                                         aCTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGcc                                    >  1:236421/1‑149 (MQ=37)                                                                                                             tAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGCCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACCCGTCCTATCAGGCCACGGCTGTTAATCGGCCttcg                                >  2:341896/1‑147 (MQ=11)                                                                                                               ggCCTGATA‑AGACGCATTACGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTa                              <  1:726841/149‑1 (MQ=255)                                                                                                                 ccTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTAcc                            <  1:336500/149‑1 (MQ=255)                                                                                                                   tGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCtt                          >  1:147284/1‑149 (MQ=255)                                                                                                                     aTA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAAGCCTGATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCtttg                        >  1:353764/1‑148 (MQ=255)                                                                                                                     aTA‑AGACGCATTACGCCTCGCATCAGGCAACGGCTGCCGGATGCGGCATCACTCCCTTATCCGACCTACGGTTCTGTTCCCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTa                        <  1:220788/149‑1 (MQ=14)                                                                                                                           aCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                   >  1:627745/1‑149 (MQ=255)                                                                                                                             gCATTACGCGTCGCATCAGGCAACGGCTGTCAGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgct                 >  1:565046/1‑149 (MQ=255)                                                                                                                              cATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgctg                <  1:667581/149‑1 (MQ=255)                                                                                                                               aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtgctgc               >  2:647826/1‑149 (MQ=255)                                                                                                                                     cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTAACTGATCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa         <  1:541442/149‑1 (MQ=255)                                                                                                                                        cgccgacggCAACGGCCGGCGGGTGCGGGGTGAACGCCTTACCCGACCTATGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa      <  2:36578/142‑1 (MQ=18)                                                                                                                                           aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc   >  1:145000/1‑149 (MQ=255)                                                                                                                                            tCAGGCAATGGCTGGCGGATGCGGCGTGGACGCCTTATCCGTCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa  <  2:333043/149‑1 (MQ=255)                                                                                                                                            tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa  >  2:456367/1‑149 (MQ=255)                                                                                                                                                 cAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa      >  1:250790/1‑140 (MQ=255)                                                                                                                                                 cAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa      <  2:250790/140‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                TGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATA‑AGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCA  >  NC_000913_3_bme_pgi/2662340‑2662626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.