Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_bme_pgi	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_bme_pgi	1299499–1300697	1300697	1–1199	79 [0]	[0] 80	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 1299498	0 (0.000)	73 (1.250)	60/290	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_bme_pgi	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_bme_pgi/1299350‑1299646                                                                                                                                                     |                                                                                                                                                     cttctCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                                     >  2:262517‑M1/1‑149 (MQ=255)   tctcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACCTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTGTGGTAGAGGAAAGTGCTAAata                                                                                                                                                   >  2:688054‑M1/1‑147 (MQ=255)      cAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                                >  2:585378‑M1/1‑144 (MQ=255)      cAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTAACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAATAATAATTAAAAAGATATTATTAATTCCAATGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                                >  2:711500‑M1/1‑144 (MQ=255)             cAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                         >  2:503575‑M1/1‑137 (MQ=255)              agttagttATAAGGATTTGCTTAACTTCTTCTCCTGACCATCATGTTATTTTGGCCACATCATAATCCTTGGCTTGCTGAAGAATAATTGAAATGATTTTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                                                                                                                        <  2:848278‑M1/150‑15 (MQ=255)              agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                                                                                                                        <  1:486703‑M1/150‑15 (MQ=255)               gttagttaTTAGGATTTCCTTAACTGCTTCTCCTCTCCTTCATGTTATTTTCGCCACATCATAATCCTTGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATAAataataatcaattgt                                                                                                                                       <  2:276568‑M1/150‑16 (MQ=255)                       taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGATGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                               >  2:908434‑M1/1‑127 (MQ=255)                        aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCAACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATAATATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                              >  2:200100‑M1/1‑126 (MQ=255)                         aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGTCTTGCTGAAGAATAATTGAAATGATATTTTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAttaataatcaattgttaaattattt                                                                                                                             >  1:651362‑M1/1‑125 (MQ=255)                         aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattg                                                                                                                             <  1:687510‑M1/150‑26 (MQ=255)                          ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCAAATAATAATCATGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAATTGATAAataataatcatttcttaaattatttt                                                                                                                            >  1:395370‑M1/1‑124 (MQ=255)                            ttttcCTTAACTGCTTCTCATCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                                                                          <  1:176483‑M1/149‑29 (MQ=255)                            aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                                                                          <  1:556963‑M1/150‑29 (MQ=255)                             tttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTTAAATGATATTATTAATTCCACTGCCTTTGGGAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                         <  1:801938‑M1/150‑30 (MQ=255)                                 cTTAACTGCTTCCCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattta                                                                                                                     >  1:624150‑M1/1‑117 (MQ=255)                                   tAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                                                                                                                   >  1:62068‑M1/1‑115 (MQ=255)                                    aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact                                                                                                                  >  2:836091‑M1/1‑114 (MQ=255)                                              cctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgttcatttcactactggaactt                                                                                                        >  1:405105‑M1/1‑104 (MQ=255)                                              cctcACCATCATGTTATTTTCGCCACATCATAATACTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                                        >  1:549464‑M1/1‑104 (MQ=255)                                               ctcACTATCATGTTATTTTAGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGACTTTGGTAGAGGAAAGTGCTAAataataatcaattgttatattattgtgcatttcactactggaactgt                                                                                                       <  2:782526‑M1/150‑48 (MQ=255)                                               ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                       <  2:899022‑M1/150‑48 (MQ=255)                                               ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataattatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                       >  2:48155‑M1/1‑103 (MQ=255)                                                tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgta                                                                                                      >  2:775673‑M1/1‑102 (MQ=255)                                                tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgta                                                                                                      >  1:690329‑M1/1‑102 (MQ=255)                                                tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgta                                                                                                      <  1:131353‑M1/150‑49 (MQ=255)                                                              ttttCTCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                        <  1:160110‑M1/150‑63 (MQ=255)                                                              ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                        <  1:348904‑M1/150‑63 (MQ=255)                                                              ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                        <  1:852531‑M1/150‑63 (MQ=255)                                                              ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgcaactgtaatcagaaaagatag                                                                                        <  1:32312‑M1/150‑63 (MQ=255)                                                               tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATTATTGAATTGATATTTTTAATTCCACTGCCTTTGGGAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                       >  2:287309‑M1/1‑87 (MQ=255)                                                               tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGATATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                       <  1:116576‑M1/150‑64 (MQ=255)                                                               tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                       >  2:152578‑M1/1‑87 (MQ=255)                                                                ttCGCCACATCATAATCCTTGGCTTGCGGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagac                                                                                      <  2:960791‑M1/150‑65 (MQ=255)                                                                ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCGTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaatactggaaatgtaatcagaaaagatagac                                                                                      >  1:847117‑M1/1‑86 (MQ=255)                                                                 tctccacaTCATTATCGTTGTCTTGTTGAAGAATAATTTAAATTTTATTATTAATTCCCATGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgtaactgtaatcagaaaagatagaca                                                                                     <  2:547531‑M1/147‑66 (MQ=255)                                                                       catcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                               >  2:457690‑M1/1‑79 (MQ=255)                                                                        atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaatatagacatgcttag                                                                              >  1:514957‑M1/1‑78 (MQ=255)                                                                        atcatAATCCTGGGCTTGATGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGATGAAAGTGCTAAataataatcaattgataaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                              <  1:192457‑M1/150‑73 (MQ=255)                                                                         tcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                             <  1:454569‑M1/150‑74 (MQ=255)                                                                          catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcc                                                                            >  2:848366‑M1/1‑76 (MQ=255)                                                                          catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcc                                                                            <  1:262517‑M1/150‑75 (MQ=255)                                                                             aaTCCTGGGCTTGCTGAAGAATAATTGATATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaat                                                                         >  1:629711‑M1/1‑73 (MQ=255)                                                                                     gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                                 <  2:551639‑M1/150‑86 (MQ=255)                                                                                         gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                             <  1:292991‑M1/150‑90 (MQ=255)                                                                                                  taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                    <  1:847636‑M1/150‑99 (MQ=255)                                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttatccaatctctatttgattgaattgaaag                                                   >  2:626677‑M1/1‑51 (MQ=255)                                                                                                   aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaag                                                   >  1:13402‑M1/1‑51 (MQ=255)                                                                                                         aaTGATATTATTAATTCCACTTCATTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                             <  1:200100‑M1/150‑106 (MQ=255)                                                                                                            gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcaaatctctatttgattgaattgaaagatgtttgtt                                          >  1:617499‑M1/1‑42 (MQ=255)                                                                                                             atatTATTAATTAAACTTCCTTTTGTAGATGAAAGTGCTAAataataatcaattgttaaatttttgtgcatttcactactggaactgatatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtta                                         <  2:311427‑M1/150‑110 (MQ=255)                                                                                                               attattAATTCGACTGCCTTTGGTAGTGGAATGTGCTAAataataatgaatttttaaattattgtgcatttctctactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                       <  1:711500‑M1/150‑112 (MQ=255)                                                                                                               attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                       <  1:585378‑M1/150‑112 (MQ=255)                                                                                                               attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataaacaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatatctatttgattgaattgaaagatgtttgttaag                                       >  1:796718‑M1/1‑39 (MQ=255)                                                                                                                ttattaATTCCACTGCCTTTGGTAGATGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                      <  2:196782‑M1/150‑113 (MQ=255)                                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAattataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaaatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                                    <  2:200266‑M1/150‑115 (MQ=255)                                                                                                                  attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                                    >  2:253519‑M1/1‑36 (MQ=255)                                                                                                                   ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaatttattgtgcatttcactactggaactgtaatctgaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                                   >  1:707568‑M1/1‑35 (MQ=255)                                                                                                                    taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatg                                  <  2:356205‑M1/150‑117 (MQ=255)                                                                                                                      aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaatagatagacatgcttagccaatctctatttgattgatttgaaagatgtttgttaaggcatgga                                >  2:299786‑M1/1‑32 (MQ=255)                                                                                                                          cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                            <  2:624150‑M1/150‑123 (MQ=255)                                                                                                                            cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagataagatagacatgcttagcaaatctctatttgattgaattgaaagatgtttgttaagtcatggatgcaag                          >  1:176207‑M1/1‑26 (MQ=255)                                                                                                                                 tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata                     <  1:503575‑M1/150‑130 (MQ=255)                                                                                                                                  ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                        <  2:206395‑M1/114‑95 (MQ=255)                                                                                                                                  ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                        >  1:206395‑M1/1‑20 (MQ=255)                                                                                                                                          ggAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgattagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat            >  1:443443‑M1/1‑12 (MQ=255)                                                                                                                                            aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatac          <  1:848366‑M1/150‑141 (MQ=255)                                                                                                                                            aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatac          >  2:303137‑M1/1‑10 (MQ=255)                                                                                                                                             aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg         <  1:152578‑M1/150‑142 (MQ=255)                                                                                                                                             aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg         <  1:287309‑M1/150‑142 (MQ=255)                                                                                                                                                tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtc      <  1:908434‑M1/150‑145 (MQ=255)                                                                                                                                                 gCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggaatggatgcaagctatagattctgatacggtca     >  1:801384‑M1/1‑5 (MQ=255)                                                                                                                                                  cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa    >  2:408134‑M1/1‑4 (MQ=255)                                                                                                                                                  cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa    >  2:488040‑M1/1‑4 (MQ=255)                                                                                                                                                   tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaat   >  1:860508‑M1/1‑3 (MQ=255)                                                                                                                                                   tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaat   >  2:344423‑M1/1‑3 (MQ=255)                                                                                                                                                   tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaat   >  2:236745‑M1/1‑3 (MQ=255)                                                                                                                                                    aaataataatcaattgttaaataattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaata  <  2:125578‑M1/150‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG  >  NC_000913_3_bme_pgi/1299350‑1299646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑