Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,7960CG36.2% 135.3 / 114.0 105A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (28/39);  new base G (20/18);  total (48/57)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 107 total aligned reads displayed.

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCG  >  NC_000913_3_bme_pgi/526649‑526940
                                                                                                                                                   |                                                                                                                                                 
ttGCGCAGGGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                  <  2:1293534/149‑1 (MQ=255)
   cgcAGCGCGGGCGGACGGTGGAGCAACGGGCCAGCCAGGTGGAGCCGGAATACACCCCGGCGCGAAAAGCTCATCTTTATCACTGCGGCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGag                                                                                                                                               <  2:797832/149‑2 (MQ=11)
    gcAGTGCGGGCTGACGGTGGAGCAACTGGCGAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCAACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAAGACGGCGTGGAGCGCGGAat                                                                                                                                              >  2:416537/1‑149 (MQ=255)
    gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAat                                                                                                                                              >  1:958815/1‑149 (MQ=255)
      aGTGCGGGGTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                            <  2:252798/149‑1 (MQ=255)
      aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                            >  1:1014435/1‑149 (MQ=255)
      aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                            >  1:1246750/1‑149 (MQ=255)
        tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                          <  1:1210178/149‑1 (MQ=255)
        tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGACGGCAGCTCTTCTCAGCGAAGACGGCACTACGGCGTGGAGCGCGGAATgtga                                                                                                                                          >  2:139826/1‑149 (MQ=17)
        ggCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                          <  1:60418/148‑1 (MQ=255)
             gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTAtgatgagt                                                                                                                                     >  1:571993/1‑147 (MQ=255)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                   <  1:518451/149‑1 (MQ=255)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                   <  2:344600/149‑1 (MQ=255)
               tGACGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                   <  1:415760/149‑1 (MQ=11)
                taCGGTTGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                  <  1:745023/148‑1 (MQ=11)
                ggcGGGGGAGCGAGTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                  <  2:253363/147‑1 (MQ=11)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGTGGCTTCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                  <  1:705629/149‑1 (MQ=11)
                 aCGGTGGAGCAACTGGCCAGAAAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                 <  1:1163754/149‑1 (MQ=255)
                     tGGAGCAACTGGCCAGACAGGTGGAGCCGTAATACACACCGGCGTGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                             <  1:747125/149‑1 (MQ=255)
                     gcgAGCAACTGGCCAGACAGCTGGAGCCGTACTACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACTGGGGTCTGCCGCTGTCGCTTATCTGCGAAGACGGCAATACGGCGTGGTGCGCGGAATATGCTGAATGGGGCAAc                                                                                                                             <  1:1087601/147‑1 (MQ=14)
                       gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACGCCGGCGCGAAAAGCTCATCTTTATCACTACGACCACCGGGGACTGCCGCTGGCGCCTATCCGCGAAGACGGCAATACGGAGGGGCGAGGGGAGCATGATGACTGGGGCAAACa                                                                                                                           >  2:849206/1‑149 (MQ=11)
                       gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                           >  1:571595/1‑149 (MQ=255)
                         gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                         >  1:398939/1‑149 (MQ=255)
                         gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCACCGGATCCGGGAATACGGCGTGGCGGGGGGGGTATGATGAATGGGGCAGCCAga                                                                                                                         >  1:251438/1‑148 (MQ=11)
                           aaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCtt                                                                                                                       >  1:970449/1‑149 (MQ=255)
                             cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGGGAAAAGATAATATTTATGACTGCGACCAAAGGGGACTGCAGCAGGAGCTTATCAGCGAAGACGGCAATAAGGCGTGGAGGGCGGAATATGATGAATGGGGGAAGCAgactaa                                                                                                                     >  2:1284789/1‑144 (MQ=14)
                             cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                     <  1:479862/149‑1 (MQ=255)
                             cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                                     >  1:1247181/1‑149 (MQ=255)
                             cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                                     <  1:823654/149‑1 (MQ=255)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCGGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGTCAACCAGCTTAATga                                                                                                                  >  1:113616/1‑149 (MQ=11)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                  >  1:105077/1‑149 (MQ=255)
                                 ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                 <  1:1062377/149‑1 (MQ=255)
                                 ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                 <  1:1116028/149‑1 (MQ=255)
                                 ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                 <  2:989505/149‑1 (MQ=255)
                                  cAGATAGGTGGAGCCGGAATACACACCGGCGGGAAGGGCGCATGTGTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                <  1:33137/149‑1 (MQ=1)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                >  1:540311/1‑149 (MQ=255)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                >  2:715163/1‑149 (MQ=255)
                                   aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCCACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGAGAAGCGAGGGGAGGATGAGGAATGGGGCAACCAGCTTAATgaggc                                                                                                               >  1:245533/1‑148 (MQ=11)
                                     aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCCGGCGTGTCGCGGGGAGTATGATGAATGGGGCAACCAGCTTCAGGAGggga                                                                                                             >  1:512514/1‑149 (MQ=11)
                                       aGGTGGAGCCGGAATACACACCGGCGCGCAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCACACCCTTCCATACGGCGTGGCGCGGGGAGGATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                           >  1:1300868/1‑149 (MQ=11)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGTACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                         >  2:702906/1‑149 (MQ=11)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                         <  1:441950/149‑1 (MQ=255)
                                          tGGAGCCGGAATAGCCAGCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTCGCGCTTATCAGCGAAGACGTCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                        <  1:86565/149‑1 (MQ=17)
                                          tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                        <  2:571595/149‑1 (MQ=255)
                                          tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                        <  1:1204326/149‑1 (MQ=255)
                                            gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAACAGCTTAATGAGGAGAACCCGca                                                                                                      >  1:333330/1‑149 (MQ=255)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCAGTGCGACCACAGGGGACTGCCGCCGGCTCTTATCAGCGAAGACGGCACTACGGCGTGCCGCGCGGAGTATGATGCATGAGGCAACCAGGTTCATGAGGAGAACCCAcatc                                                                                                    >  2:816645/1‑149 (MQ=14)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                    >  1:147574/1‑149 (MQ=255)
                                               ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                             <  2:214073/123‑1 (MQ=255)
                                               ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                             >  1:214073/1‑123 (MQ=255)
                                                cGGAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                                  >  1:1203644/1‑148 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                  <  2:9492/115‑1 (MQ=11)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                  >  1:9492/1‑115 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                <  2:970449/149‑1 (MQ=255)
                                                     tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgca                                                                                             <  1:788027/149‑7 (MQ=11)
                                                     tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTa                                                                                             <  1:918581/149‑1 (MQ=255)
                                                      acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                            <  2:958815/149‑1 (MQ=255)
                                                       cacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGTAGGCGGCAATACGGCGTGGAGCGGGAGATATGATGTATGGGGCAGCCAGCGTAATGGAGAGAACCCGCACCATGTTTAta                                                                                           >  2:1035034/1‑148 (MQ=255)
                                                        ccacCGGCCCGAAAAGCTCATCTTTATCACTGCGACAACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                          <  2:249065/148‑1 (MQ=255)
                                                             ggCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGt                                                                                     >  1:805541/1‑149 (MQ=255)
                                                              gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                  >  2:996219/1‑119 (MQ=255)
                                                                  caaaaGGTCGTCTTTAGCCCGGCGAACACCGGGACCTGCCGCTGGCGCCGATCAGCGAAGACGGCAATAAGGCGTGGAGGTCTGGGTGTGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGt                                                                                <  2:1208434/148‑1 (MQ=14)
                                                                   aaaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGCCGGCAGTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGGCGTACCGTc                                                                               >  1:780696/1‑149 (MQ=255)
                                                                    aaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                              <  1:211953/149‑1 (MQ=255)
                                                                     aaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTg                                                                             >  1:556896/1‑149 (MQ=255)
                                                                      tgCTCATCTTTATCACGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCTTCATGTGTATCAGCCGTACCGTCTGc                                                                            <  2:225706/148‑1 (MQ=255)
                                                                        cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                  >  2:57265/1‑125 (MQ=255)
                                                                        cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                  <  1:57265/125‑1 (MQ=255)
                                                                        cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACGGTCTGCCa                                                                          >  1:180445/1‑149 (MQ=255)
                                                                         tCATGTTTATCACTGCGCCCGCCGGGGACTGCCGCTGGCGATTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                         <  2:562994/149‑1 (MQ=255)
                                                                             cTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                         <  2:56032/81‑1 (MQ=11)
                                                                             cTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                         >  1:56032/1‑81 (MQ=11)
                                                                              tttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                            <  1:866339/77‑1 (MQ=255)
                                                                              tttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                            >  2:866339/1‑77 (MQ=255)
                                                                               ttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGag                                                                                                                                               <  1:644394/73‑2 (MQ=11)
                                                                               ttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGag                                                                                                                                               >  2:644394/1‑72 (MQ=11)
                                                                                    aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGGCGGCAATACGGCGTGGAGCGGGGAGTCTGGTGAATGGGGCGACCAGCTTAAAGAGGAGAACCCGCATCCCCTGCGCCAGCCGTACGGGCTGCGCGGgcagcagact                                                              >  2:183353/1‑146 (MQ=255)
                                                                                    aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑Cat                                                             <  1:1309571/149‑1 (MQ=255)
                                                                                     cTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCAGTACCGTCTGCCAGGGCAGCAG‑Catg                                                            >  1:1115360/1‑149 (MQ=255)
                                                                                         gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CAtgatga                                                        <  2:1014435/149‑1 (MQ=255)
                                                                                         gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CAtgatga                                                        <  1:1134810/149‑1 (MQ=255)
                                                                                              ccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAAt                                                   <  1:139826/149‑1 (MQ=255)
                                                                                                tgggTCTGCCGGTGGCGGTTATCAGGGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCa                                                 <  2:589119/148‑1 (MQ=255)
                                                                                                 gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAg                                                >  2:1015361/1‑149 (MQ=255)
                                                                                                  ggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAga                                               >  2:856886/1‑148 (MQ=255)
                                                                                                   gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAggg                                              <  1:999526/149‑1 (MQ=255)
                                                                                                   gACGGCCGCTGGCGGTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAggg                                              <  1:1231058/149‑1 (MQ=255)
                                                                                                      tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAGTCAGGGCTg                                           >  2:124109/1‑149 (MQ=255)
                                                                                                            tGGCGCGTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACtat                                     <  1:588906/149‑1 (MQ=255)
                                                                                                             ggCGCTTAGCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACtata                                    <  1:1093980/149‑1 (MQ=255)
                                                                                                              gcgcTTATCAGCGAAGACGGCAAGACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATaa                                   <  2:344076/149‑1 (MQ=255)
                                                                                                                gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCCGGAG‑CATGATGAGGCAGCAGGGCTGTACTATAAcc                                 >  1:2070/1‑149 (MQ=255)
                                                                                                                gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAC‑CATGATGAGGAATCAGGGCTGTACTATAAcc                                 >  2:954286/1‑149 (MQ=255)
                                                                                                                      cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGTAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGGCGCCATCCT‑TGGAGTCAGGGCTGTACTATAACCGTCAcc                           <  2:1280940/149‑1 (MQ=17)
                                                                                                                       aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                          <  2:1161093/149‑1 (MQ=255)
                                                                                                                        gCGAGGACGGAAATAGTGCGTGGATCGCGTAATAGGAAGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAGGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGCCACCgg                         <  2:541793/149‑1 (MQ=17)
                                                                                                                                  cAATATGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc               >  2:741317/1‑149 (MQ=255)
                                                                                                                                         gcttGGAGCGCGGAATATGATGATTGGTGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCa        <  1:336954/146‑1 (MQ=255)
                                                                                                                                          cGTGGAGCGCGGAATATGATGAAGTGGGCAACCAGCTTAATGAGGAGACCCTGCTCCCCCGCTATTAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAg       <  1:640808/149‑1 (MQ=14)
                                                                                                                                               agggcgGAATAGGAGGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCg  <  2:4615/146‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAG‑CATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCG  >  NC_000913_3_bme_pgi/526649‑526940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑