New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 864990	54 (0.920)	3 (0.050)	3/288	7.8	5.4%	coding (140/750 nt)	moeB	molybdopterin synthase sulfurylase
	?	NC_000913_3_bme_pgi	= 865073	52 (0.920)					coding (57/750 nt)	moeB	molybdopterin synthase sulfurylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCT  >  NC_000913_3_bme_pgi/864841‑865138                                                                                                                                                      |                                                                                                                                                     tCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGCTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCg                                                                                                                                                      <  1:92787/150‑1 (MQ=255)      gTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCt                                                                                                                                                 <  2:254227/150‑1 (MQ=255)       gcAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTg                                                                                                                                                <  1:459495/149‑1 (MQ=255)         cgggCGGCACGGGCGGATTCCACGTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGGCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGca                                                                                                                                              <  2:302765/149‑1 (MQ=255)          gggCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGcac                                                                                                                                             <  1:38822/150‑1 (MQ=255)           ggCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGcaca                                                                                                                                            <  1:602521/150‑1 (MQ=255)                    ggCGGATTCCACCTTCGGTTGCGCGCCCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGGCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCAGAGCCGAGgcc                                                                                                                                   <  2:210146/150‑1 (MQ=255)                        gATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGCGGCCGccc                                                                                                                               >  2:360551/1‑150 (MQ=255)                         aatCCACCTTCGGTTTCCCGAACGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTTCCAGATACTGAGAGGCTGCACAGCCGAGGCCGCCCa                                                                                                                              <  2:631226/148‑1 (MQ=255)                             cACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGcc                                                                                                                          <  2:126153/150‑1 (MQ=255)                               ccTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCa                                                                                                                        >  1:330662/1‑150 (MQ=255)                                 ttCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACt                                                                                                                      <  1:595107/150‑1 (MQ=255)                                    ggTTGCCCGACCGTGGCATCACTGTGGAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTGCCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGACGCCGCCCAGGCCCCCTATc                                                                                                                   >  2:233906/1‑150 (MQ=255)                                     gTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTACACAGCCGAGGCCGCCCAGGCCCACTATCa                                                                                                                  <  1:379040/150‑1 (MQ=255)                                        gCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCa                                                                                                               <  1:38020/150‑1 (MQ=255)                                         cccGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCa                                                                                                                        <  2:814440/140‑1 (MQ=255)                                         cccGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCa                                                                                                                        >  1:814440/1‑140 (MQ=255)                                          ccGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTAACATCAcg                                                                                                             >  2:393731/1‑150 (MQ=255)                                             aCCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCga                                                                                                          <  1:264964/150‑1 (MQ=255)                                              ccGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGTCCACTATCAGCACGCgag                                                                                                         >  1:196702/1‑150 (MQ=255)                                              ccGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCgag                                                                                                         <  2:927865/150‑1 (MQ=255)                                                 tGGCATCACTGTGCAGTGGCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAAt                                                                                                      <  2:758039/150‑1 (MQ=255)                                                 gggCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGGCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAAt                                                                                                      <  2:762822/149‑1 (MQ=255)                                                   gCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGATCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCt                                                                                                    <  2:793818/150‑1 (MQ=255)                                                      gcACTGTGCAGTGGCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGGCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTc                                                                                                 <  1:51600/149‑1 (MQ=255)                                                          tgtgCAGTGTCTGGCGTTGCAGATTCGCGAGCGAAACCGTGTCGAAGGTGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAgcg                                                                                             <  1:309334/150‑1 (MQ=255)                                                             gCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGcct                                                                                          >  2:137707/1‑150 (MQ=255)                                                               aGTGTCTGGCGGTGCAGATTCGAGAGCGAAACCGTGTCGAAGGCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGTGcctcc                                                                                        <  2:222288/150‑1 (MQ=255)                                                                     tGGCGTTGCCGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTAGCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCg                                                                                  <  1:180877/150‑1 (MQ=255)                                                                       gCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTc                                                                                <  2:330662/150‑1 (MQ=255)                                                                            gCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAAt                                                                           <  2:785789/150‑1 (MQ=255)                                                                            gCAGATTCGAGAGCGAAACCGTGGCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAAt                                                                           <  1:877904/150‑1 (MQ=255)                                                                              aGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCa                                                                         >  2:350942/1‑150 (MQ=255)                                                                               gATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGCGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCaa                                                                        >  1:217918/1‑150 (MQ=255)                                                                                 ttCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAg                                                                      <  1:679763/150‑1 (MQ=255)                                                                                   cGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCCCAGCCGAGGCCGCCCAGGCCCACTATCAGCGCGCGAGAATCTTTCAGCGCCTCCTGGCCGTCACAATCAACGcc                                                                    >  1:528704/1‑150 (MQ=255)                                                                                      gagCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCcgcg                                                                 >  1:3363/1‑150 (MQ=255)                                                                                        ccGAAACCGTGTCGAAGTCGAGCAGCGGCAGGTTACCGACACCGGCGCTTGCCTGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGCCCCACTAGCAGCACGCGAGAATCTATCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCa                                                               <  2:932318/149‑1 (MQ=255)                                                                                           aaaCCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGaa                                                            >  1:877842/1‑150 (MQ=255)                                                                                                  gtCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGTCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTg                                                     <  2:196702/150‑1 (MQ=255)                                                                                                     gAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCCGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGCATGATTTGCCg                                                  >  2:332889/1‑150 (MQ=255)                                                                                                      aaGTAGAGCAGCGTCAGGTTACCGACACCGTCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAAGCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCgg                                                 <  1:184788/150‑1 (MQ=255)                                                                                                               agcGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAgcgc                                        <  1:739747/150‑1 (MQ=255)                                                                                                                 cggcAGGTTCCCGACACCGGCGCTTGCGAGATCCTGCGAGGCGGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCCCGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCgcag                                      <  1:694333/147‑1 (MQ=255)                                                                                                                 cGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCgcag                                      <  1:824707/150‑1 (MQ=255)                                                                                                                    cAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGTGAGAATCTTTCAGCGCCTCCTCGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCAt                                   >  1:742493/1‑150 (MQ=255)                                                                                                                        ttACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGCGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTcc                               <  2:217918/150‑1 (MQ=255)                                                                                                                          aCCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTg                             <  2:828388/150‑1 (MQ=255)                                                                                                                           ccGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGTGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGa                            >  2:890546/1‑150 (MQ=255)                                                                                                                                   ggCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAg                    <  1:350942/150‑1 (MQ=255)                                                                                                                                       gttGCCAGATACTGCGAGGGTGTACAGCCGAGGCCGCCCAGGGCCACTAGCAGGACGCGAGAATCTTTCAGGGCCTCCTGGACTTCAAATTCAAAGCCGCGAAGAATGTTTTGCCTTTTGTAGCGCAGCTTCTCCTGATCGCTTAGttgg                <  2:657147/149‑3 (MQ=255)                                                                                                                                          gCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGcc             <  2:858095/150‑1 (MQ=255)                                                                                                                                                  cTGCGAGGCTGCTCAGCCGAGGCCGCCCAGGCCGACTATCTGCAGGCGAGATTCTTTCAGCGCCTCCTGGCCGTCAAAATCAAATCCGCGCAGAATCATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAgg     <  1:233906/150‑1 (MQ=255)                                                                                                                                                    gCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGcc   <  2:3363/150‑1 (MQ=255)                                                                                                                                                     cGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCACAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCCGCATCTCCTGATCGCGGAGTTCCGCCATTACAGGCCt  >  1:880167/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                     TCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCT  >  NC_000913_3_bme_pgi/864841‑865138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.