Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,8000AG41.9% 29.3 / 34.5 43E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (12/13);  new base G (9/9);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_bme_pgi/526652‑526948
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cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                      <  1:34290/149‑1 (MQ=255)
 gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAat                                                                                                                                                     >  1:160819/1‑149 (MQ=255)
      gCGGGCTTGCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                                <  1:661772/149‑1 (MQ=11)
         ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGGGTATGATGaa                                                                                                                                             >  1:596789/1‑149 (MQ=255)
          gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                            >  1:20509/1‑149 (MQ=255)
             gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                         <  1:302487/149‑1 (MQ=255)
             cacgggtgAGCAACTGGCCAGACAGGTGGAGCCGCAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                         <  2:288598/142‑1 (MQ=11)
                  tGGAGTAACTGGCCAGACAGGTGGAGCCTGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                    <  1:24634/149‑1 (MQ=11)
                  tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACAGCGCGGCGCGGGGGGTATGATGAATGGGGCagc                                                                                                                                    >  2:229597/1‑147 (MQ=11)
                     cgcctttGGCCAGACAGGTGCGGCCCGAATACACACCGGTGCGAAAAGCTCATCTTTATCACTGCGGCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                                                                 <  2:659839/143‑1 (MQ=17)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACGGCGTGGCGCGCGGAGTATGATGAAGGGGGCAACCAGc                                                                                                                                >  2:568459/1‑149 (MQ=255)
                       cAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCt                                                                                                                               <  2:431485/149‑1 (MQ=255)
                        aaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGACGACGGCAATACGGCGCGGCGCGGGGAGGATGCTGACTGGGGCGACCAGcct                                                                                                                              >  2:785093/1‑147 (MQ=11)
                           tGGCCAGACAAGTGGAGCCGGAAAACACACCGGCGCGAAAAGCTCATCTATATCACCGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAACACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                           <  2:403394/149‑1 (MQ=11)
                           gggCCAGACAGGTGGAGGCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                           <  1:125837/148‑1 (MQ=255)
                              acAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                        <  2:736424/148‑1 (MQ=255)
                               gaGACAGGTGGAGCCGGAATACTAACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCCGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                       <  1:1224/148‑1 (MQ=11)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                      >  1:485249/1‑149 (MQ=255)
                                 gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgaggag                                                                                                                     >  1:617698/1‑149 (MQ=255)
                                    aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCACTACGGCATCGAGCGCGGAATATGAGAAAGGGGGGAGCAAGCATAATGAGGAGAAc                                                                                                                  >  2:123245/1‑149 (MQ=17)
                                       tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGGGAACCCg                                                                                                               >  1:305281/1‑149 (MQ=255)
                                        ggAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGc                                                                                                              <  1:64059/149‑1 (MQ=255)
                                         gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                             >  1:752049/1‑149 (MQ=255)
                                           gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                           >  1:412056/1‑149 (MQ=255)
                                            ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                                          >  1:444820/1‑149 (MQ=255)
                                              ggAATACACACCGGCGCGAAAAGCTCATCTTTATCCCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtg                                                                                                        <  1:229862/149‑1 (MQ=255)
                                                   acacacCAGCGAGAAAAGCTCATCTTTATCACTGAGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                                   <  2:450343/149‑8 (MQ=11)
                                                           gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGCGGAGCGCGGAATATGATGAATGGGGCCACCAGCTTAATAAGGCGAACCCGCATCATGTGTGTCCGCCGTa                                                                                           >  2:508601/1‑149 (MQ=18)
                                                               gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGt                                                                                       >  1:251271/1‑149 (MQ=255)
                                                                                 aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGc                                                                                               >  1:453905/1‑123 (MQ=255)
                                                                                 aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGc                                                                                               <  2:453905/123‑1 (MQ=255)
                                                                                   tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGCAAGATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                                   <  1:788668/149‑1 (MQ=255)
                                                                                       accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                               <  1:139798/149‑1 (MQ=255)
                                                                                           ccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAt                                                           >  1:121520/1‑149 (MQ=255)
                                                                                                gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                      <  2:104784/149‑1 (MQ=255)
                                                                                                gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                      <  1:449805/149‑1 (MQ=255)
                                                                                                  cTGCCGCTGGCGCTGATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCt                                                    <  2:160819/149‑1 (MQ=255)
                                                                                                          ggCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACtata                                            >  2:232665/1‑149 (MQ=255)
                                                                                                             gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                         <  1:717711/149‑1 (MQ=255)
                                                                                                                     gCGAAGACGGCACTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAGTGAGGAGAACCCGCCCCATGTATATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGCGGCGGCATCCGGCCTGGCCCGTAGCCGTCACCgg                                 >  2:258965/1‑149 (MQ=17)
                                                                                                                     gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                                 >  1:290278/1‑149 (MQ=255)
                                                                                                                       gAAGACGGCAATACGGAGTGGAGCGAGGAATCTGATGAATGGGGCCAACAGCTTACTGAGGGGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCAAGGGCAGCAGCATGATGCGTCATTAGGCCTAGACTATCACCGTccctgtta                               >  2:774176/1‑142 (MQ=255)
                                                                                                                                  tACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt                    <  1:579776/149‑1 (MQ=255)
                                                                                                                                     ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCATACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc                 >  2:732333/1‑149 (MQ=255)
                                                                                                                                           gagggaggAATATGGTGAATGGGGCAACAAGATTAATGAGGAGAACCGCCATCATGTGTATAAGCCGTACCGTCTGCCAGAGCAGCAGTATGATGAGGAATCAGGGCTGTACTATAACCGTCCCCGGTACGACGATCCGTGGTAGGGGc           <  2:374640/143‑1 (MQ=255)
                                                                                                                                                    atatGATGAAGGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGGGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGGGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTa  <  2:378442/149‑1 (MQ=255)
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CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_bme_pgi/526652‑526948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑