Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi526,8000AG21.8% 159.8 / 42.9 88E1180E (GAA→GAGrhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (41/27);  new base G (9/10);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATAT  >  NC_000913_3_hsa_pgi/526652‑526946
                                                                                                                                                    |                                                                                                                                                  
cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                    <  1:1227166/149‑1 (MQ=255)
cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                    <  1:205437/149‑1 (MQ=255)
cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                    <  1:714644/149‑1 (MQ=255)
 gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAat                                                                                                                                                   >  1:673360/1‑149 (MQ=255)
  cAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGCAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAGACGGCAATACGGCGGGGAGCGAGGAata                                                                                                                                                  >  1:577385/1‑149 (MQ=255)
   aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                                 >  1:111536/1‑149 (MQ=255)
   aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                                 <  2:435223/149‑1 (MQ=255)
     tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                               >  1:717042/1‑149 (MQ=255)
      gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGATGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                              <  1:462444/149‑1 (MQ=255)
      gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                              <  2:190995/149‑1 (MQ=255)
       ctggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCTCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgatg                                                                                                                                             <  1:401722/147‑1 (MQ=255)
          gCTGACGTTGGAGCAACGGGCCAGCCCGGTGGAGACGTTATACACACCGGCGCGAAAAGCTCTTGTTTATCACTGCGACCACCGGGGACTGCCTCTGGCGCTTATCAGCGAAGACGGAAATACGGAGTGGCGCGGGGAGTATGATGAAt                                                                                                                                          <  2:988938/149‑1 (MQ=11)
              aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACAGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                      <  1:1163668/149‑1 (MQ=255)
                 gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGAGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCTCTGGCCCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCaa                                                                                                                                   >  2:85407/1‑149 (MQ=255)
                  tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                  <  2:351051/149‑1 (MQ=255)
                    gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACCGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCa                                                                                                                                <  2:404883/149‑1 (MQ=255)
                     aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAg                                                                                                                               >  2:727045/1‑149 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATTCGGCGTGGAGCGCGGAAGATGATGAATGGGGCAACCAGc                                                                                                                              >  1:885997/1‑149 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                              >  2:445620/1‑149 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                              <  1:1033218/149‑1 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                              >  1:244408/1‑149 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                              >  1:65803/1‑149 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCCTGGAGCGCGGAATATGATGAAAGGGGCAACCAGc                                                                                                                              >  1:942367/1‑149 (MQ=255)
                       cAACTGGCCAGACAGGGGTAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTTGAGCGCGGAATATGATGAATGGGGCAACCAGCt                                                                                                                             <  2:1024534/149‑1 (MQ=255)
                         aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTa                                                                                                                           >  2:551419/1‑149 (MQ=255)
                         aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                           <  1:41342/149‑1 (MQ=255)
                           tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                         <  2:1400634/149‑1 (MQ=255)
                           tGGCCAGACAGGTGGAGCCGGAATACACACCGGAGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACCGCAATACGGCGTGGCGCGGGGAGTAAGATGATTGGGGCAACCCGCATatt                                                                                                                         >  2:107034/1‑147 (MQ=11)
                             gCCAGACAGGGTTAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCTGGGACTGCCGCTGGCGCTTATCAGCGAAGTCGGCATTACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCATCTTAATga                                                                                                                       <  2:70768/149‑1 (MQ=11)
                               cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGATCATCTATATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                     <  1:1361229/149‑1 (MQ=11)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAATGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                    <  2:70409/149‑1 (MQ=255)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                    <  1:971193/149‑1 (MQ=255)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                    <  1:1505918/149‑1 (MQ=255)
                                  aCAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATATGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGgaga                                                                                                                  >  1:1204643/1‑149 (MQ=255)
                                    aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAATACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                >  2:1324447/1‑149 (MQ=255)
                                    aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                >  2:1330509/1‑149 (MQ=255)
                                      gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGCCGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTATTGAGGAGAAccc                                                                                                              >  2:1227117/1‑149 (MQ=11)
                                      gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                              >  1:271506/1‑149 (MQ=255)
                                       tGGAGCCGTAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCTGGGACTGCCGCTGGCGCTTATCAGCTAAGACGGCAATAAGGCGTTGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                             <  1:962464/149‑1 (MQ=17)
                                          aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                          >  1:423802/1‑149 (MQ=255)
                                           gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCACTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                         >  2:1071541/1‑149 (MQ=255)
                                            ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcc                                                                                                        >  1:1420733/1‑148 (MQ=255)
                                            ccGGAATACACACCGCCGCGAAAACCTCATCTTTAACACTGCGACCACCGTGGACTGCCGCTCACGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAAGGGCCAACCACCTTAAAGAGCACAACCCCcaaca                                                                                                        >  1:1438393/1‑149 (MQ=14)
                                             cGGTATACACACCGGCGCGAACCGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGTGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                       <  2:360686/149‑1 (MQ=14)
                                             cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                                       >  2:971715/1‑148 (MQ=255)
                                               gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCGGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                     <  1:1179231/149‑1 (MQ=255)
                                                aaTACATACCGTCGCGAACAGCTCATCTTTATCACTGCGACCCCCGGGGACGGCCGCTGGGGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGTTGATTGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctg                                                                                                    <  2:1282403/149‑5 (MQ=11)
                                                 aTACACACCGGCGCGAAAAGCTCATCTATATCACAGCGACCACCGGGTACTCCCGCAGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATgtgt                                                                                                   <  1:987485/149‑1 (MQ=17)
                                                   acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCCGCTTAATGAGGAGAACCCGCATCATGTGTag                                                                                                 >  2:1154958/1‑148 (MQ=255)
                                                     acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATcc                                                                                               >  2:956652/1‑148 (MQ=255)
                                                     acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGCAGCGCGGAATATGATGAAAGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATCTGTATCa                                                                                               >  1:90875/1‑149 (MQ=255)
                                                      cacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAg                                                                                              <  2:1520624/149‑1 (MQ=255)
                                                        ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCAGAATCATGTGTATCAgac                                                                                            >  1:32405/1‑147 (MQ=255)
                                                         cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCATCCg                                                                                           >  2:136639/1‑149 (MQ=255)
                                                             gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                       >  2:720158/1‑149 (MQ=255)
                                                               gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCTGAATATGATGAATGGGGCCACCAGCTTAAGGAGGAGAACCCGCAGCATGTCTATCAGCCGAACCGt                                                                                     >  2:1073495/1‑149 (MQ=18)
                                                                     gcgatacttATCCCTGGGACCCCCCGGGTCTGCCGCTGGCGCTTATCAGCGAATCCGGCAATACGGCGGGGAGCGCGGTATATGTTGCATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                               <  2:1033213/142‑1 (MQ=17)
                                                                      tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                              >  1:207762/1‑149 (MQ=255)
                                                                        aTCTTTATCACTGCGACCACCGGGGACTTCCGATGTCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGGCGTACCGTCTGCCAggg                                                                            <  1:1429318/149‑1 (MQ=255)
                                                                         tCTTTATCACTGCGCCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGATTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgc                                                                           >  2:67794/1‑149 (MQ=255)
                                                                         tCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgc                                                                           >  1:618313/1‑149 (MQ=255)
                                                                           tttATCACTGCGACCACCGGGGACTGCCGCTGGCGATTATCAGCGAAGACGGCAATACCGCGTGGAGCGCGGAAAATCATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCATCTGTATCAGCCCAACCGTCTCCCAGGgcac                                                                         >  2:811169/1‑148 (MQ=17)
                                                                                   tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                                 >  1:790017/1‑149 (MQ=255)
                                                                                     cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCACTACGGCGGGGAGCGCGGAAGAAGATGAAGGGGGCAACCCGCTTAAGGAGGAGAACCCGCATCATGTGGATCCGCCGGACCGTCAGCCACGGCAGCAGCatgagg                                                               >  2:1148814/1‑147 (MQ=255)
                                                                                     cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                               >  1:555804/1‑149 (MQ=255)
                                                                                          accGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGaa                                                          >  2:785788/1‑149 (MQ=255)
                                                                                            cGGGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATc                                                        >  1:15055/1‑149 (MQ=255)
                                                                                                gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                    <  2:568950/149‑1 (MQ=255)
                                                                                                   tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                                 >  1:1181267/1‑149 (MQ=255)
                                                                                                   tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                                 >  2:304551/1‑149 (MQ=255)
                                                                                                     ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                               >  2:542159/1‑149 (MQ=255)
                                                                                                     ccGCTGGCGCTTATCAGCGAAGACGGCAAGACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                               <  2:1405146/149‑1 (MQ=255)
                                                                                                        tctttgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCGGGGCAGCAGCATGATGAGGAATCAGGGCTGTACta                                            >  2:176382/6‑149 (MQ=255)
                                                                                                        cTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCTACCAGCTTAATGAGGAGAACCCGCATCATGTGTATAAACCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACta                                            <  2:233153/149‑1 (MQ=255)
                                                                                                             gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                       >  1:373195/1‑149 (MQ=255)
                                                                                                             gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGAATGATGAGGAATAATGGCTGTACTATAAcc                                       >  2:309466/1‑149 (MQ=255)
                                                                                                                  tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                                  >  2:1089121/1‑149 (MQ=255)
                                                                                                                     gCGAAGCCGGCAATATGGCGTGGAGCGCGGAATATGATGAATCGGGCAACCAGCTTAATGAGGAGAACCCGCCTCATGTGTATCATCCGTACCGTCTGCCACGGCAGCAGCATGATGACGAATCAGGGCTGTACTAAAACCGTCACCgg                               >  2:1042236/1‑149 (MQ=17)
                                                                                                                     gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                               >  2:328732/1‑149 (MQ=255)
                                                                                                                     gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCCTCAcccg                               >  2:1101929/1‑147 (MQ=255)
                                                                                                                       gAAGACGGCAATATGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                             <  2:1204643/149‑1 (MQ=255)
                                                                                                                         aGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGCGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtact                           >  2:1301748/1‑149 (MQ=255)
                                                                                                                          gACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGAAACCAGCTTAATGAGGAGAACAAGCATCATGTGTATCAGCCGTACCGTATGCCAGGGCAGCAGCAGGATGAGGAATCAGGTCTGTACTATAACCGTCACCGGgacta                          >  2:968531/1‑149 (MQ=17)
                                                                                                                             ggCAATACGGCGTGGAGCGCGGAATTTGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTCCCGTCTGCCAGGGCCGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGa                       >  2:740635/1‑149 (MQ=255)
                                                                                                                               cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCTGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                     <  1:85407/149‑1 (MQ=255)
                                                                                                                               cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                     >  2:50770/1‑149 (MQ=255)
                                                                                                                                 aTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCg                   <  2:15055/149‑1 (MQ=255)
                                                                                                                                  tACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt                  <  1:369297/149‑1 (MQ=255)
                                                                                                                                     ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTGTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc               <  1:136639/149‑1 (MQ=255)
                                                                                                                                     ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc               >  1:408605/1‑149 (MQ=255)
                                                                                                                                     ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAGCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc               <  1:720158/149‑1 (MQ=255)
                                                                                                                                          ggAGCGAGGAATATGATGAATGGTTCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCTTGTTGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg          <  2:673360/149‑1 (MQ=17)
                                                                                                                                              cgcgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGt      <  1:785788/149‑1 (MQ=255)
                                                                                                                                               gcgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGta     <  2:244408/149‑1 (MQ=255)
                                                                                                                                                  gAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTatat  <  2:423802/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATAT  >  NC_000913_3_hsa_pgi/526652‑526946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: