Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	3,823,987	G→T	G530C (GGT→TGT)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	3,823,987	0	G	T	100.0%	222.4 / NA	71	G530C (GGT→TGT)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/37);  total (34/37)

TTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTC  >  NC_000913_3_hsa_pgi/3823842‑3824131                                                                                                                                                  |                                                                                                                                                 ttCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTa                                                                                                                                               <  1:372553/149‑1 (MQ=255)   cTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAAc                                                                                                                                             >  1:63379/1‑149 (MQ=255)   cTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAAc                                                                                                                                             <  2:315062/149‑1 (MQ=255)       aaGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCaa                                                                                                                                         <  2:16852/149‑1 (MQ=255)          ccTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGa                                                                                                                                      >  1:1429659/1‑149 (MQ=255)          ccTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGAAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCGTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGa                                                                                                                                      >  2:502670/1‑149 (MQ=255)              ggCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCgt                                                                                                                                  >  1:142194/1‑149 (MQ=255)               gCCGTCGTCTGCTCAACCATGCTTTGGGTGGTTGCCGTAAGCTGTCTGAAATCCCGCAGGAAAATATTCATCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCgtg                                                                                                                                 <  1:24706/149‑1 (MQ=255)                ccGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCgtgg                                                                                                                                <  2:529935/149‑1 (MQ=255)                  gtcgtcTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCgtggtg                                                                                                                              <  2:142194/149‑1 (MQ=255)                      tcTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCTATGAGCGTGGTGGTcg                                                                                                                          >  1:547461/1‑149 (MQ=255)                         gCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGa                                                                                                                       <  2:592517/149‑1 (MQ=255)                          cgcAACCATGCTTTGGGTGGTAGCCGTAAGCTGACTGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGaa                                                                                                                      <  2:1013374/147‑1 (MQ=255)                           tCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAATCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGaaa                                                                                                                     >  2:963431/1‑149 (MQ=255)                             aaCCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGaaaaa                                                                                                                   >  2:446187/1‑149 (MQ=255)                              aCCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAAt                                                                                                                  <  2:774470/149‑1 (MQ=255)                               ccATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATc                                                                                                                 >  1:1481006/1‑149 (MQ=255)                                cATGCGTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGTAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGGGGGGGTCGCGAAAAATCt                                                                                                                <  1:1518313/149‑1 (MQ=255)                                 aTGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATAATCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGGAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTg                                                                                                               <  1:1346277/149‑1 (MQ=255)                                  tGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGc                                                                                                              <  2:1198259/149‑1 (MQ=255)                                   gCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTAAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACTATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCa                                                                                                             >  1:1467007/1‑149 (MQ=255)                                    cTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCaa                                                                                                            >  1:467101/1‑149 (MQ=255)                                         ggtggtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATgg                                                                                                       <  1:255423/149‑1 (MQ=255)                                          gtggtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATggg                                                                                                      <  2:63379/149‑1 (MQ=255)                                           tggtAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTTGTCGCGAAAAATCTGCAACATgggg                                                                                                     <  1:26316/149‑1 (MQ=255)                                           tggtAGCCGTAAGCGGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATgggg                                                                                                     <  1:876018/149‑1 (MQ=255)                                               agacGTAAGCTGAATGAACTACCCGAGGAAAATATTCAGCGCGATCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACAATGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGc                                                                                                 <  1:985479/146‑1 (MQ=255)                                               aGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCATCATGGGGACGc                                                                                                 >  1:900411/1‑149 (MQ=255)                                                 ccGTAAGCTGAATGAAATCCCGCAGGAATATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCt                                                                                               <  1:570928/149‑1 (MQ=255)                                                      aGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCAtt                                                                                          <  1:1514883/149‑1 (MQ=255)                                                       gCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTCGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTc                                                                                         >  2:826853/1‑149 (MQ=255)                                                        cTGAATGAAATCCCGCAGGAAAATATTCAGCGCGTGCTGGATCGCTTGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTcc                                                                                        >  1:59433/1‑149 (MQ=255)                                                         ctcatgaaATCCCGCAGGAAAATATTCACCGCCAGCTGGATCGCATGAAGCGGGCACCGCTTGACGAGCTGCTGGCCGAAATCGGACTTTGTAACGCAATGAGCGGGGTGGGCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCa                                                                                       <  1:669305/146‑1 (MQ=255)                                                          gaatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAc                                                                                      <  1:1246644/149‑1 (MQ=255)                                                           aatgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCTGACTTTGTAACGCAATGAGCGTTGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAcc                                                                                     <  1:110452/149‑1 (MQ=255)                                                            atgaaCTCCCGCAGGAAAATAATCAGCGCGAGCTGGATCGCTTGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACATTGTAACTCAATGAGCGTGGTTGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAcct                                                                                    <  2:1392354/149‑2 (MQ=255)                                                            atgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCg                                                                                    >  2:529151/1‑149 (MQ=255)                                                             tgaaATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCgg                                                                                   >  1:820075/1‑149 (MQ=255)                                                              gaaATACCGCAGGAAAATATTCAGCGCGAGCTGCATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTCGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGc                                                                                  >  1:227095/1‑149 (MQ=255)                                                                  tacggCAGGAAAATAATCAGCGCGCGCTGGATCGCAGGAAGCTGGCAACGCGTGACGATCTGCTGTCAGAAATCGGACTATGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCCACATGGGGACGCCTCCATTCCACCGGCAAcc                                                                              <  2:990751/145‑1 (MQ=255)                                                                        aGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTCGCAGAAATCGGAATTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACAAGGGGACGCCTCCATTCCACCGGCACACCAAAGc                                                                        >  2:706592/1‑149 (MQ=255)                                                                          gAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCCCCCAAAAGCCa                                                                      >  1:1321860/1‑149 (MQ=255)                                                                            aaatcatCAGCGAGAGCTGGATCGCATGACGCTTGCAACGCTTGACGATCTGCTGGCAGAAATCGGTCTTTGTAAGGCAATGAGAGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCAGCGGCAACCCAAAGCCACg                                                                    <  1:282618/143‑1 (MQ=255)                                                                            aaaTATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACg                                                                    >  2:159653/1‑149 (MQ=255)                                                                              atatTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGa                                                                  <  2:467101/149‑1 (MQ=255)                                                                                 ttCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACAt                                                               <  1:1168601/149‑1 (MQ=255)                                                                                     gcgcGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGc                                                           >  1:1345158/1‑149 (MQ=255)                                                                                           gCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCAtaa                                                     <  2:900411/149‑3 (MQ=255)                                                                                           gCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTa                                                     >  2:944749/1‑149 (MQ=255)                                                                                            cTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTaa                                                    >  1:519068/1‑149 (MQ=255)                                                                                            cTGGATCGCATGAAGCTGGCAACACTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTaa                                                    >  1:562869/1‑149 (MQ=255)                                                                                             tGGATCGCATGAAGCTCGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTaaa                                                   <  2:449752/149‑1 (MQ=255)                                                                                              ggATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                  <  2:1020302/149‑1 (MQ=255)                                                                                              ggATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                  <  1:529151/149‑1 (MQ=255)                                                                                              ggATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTCCAACATGGCGACGCCACCATTCCACCGGCACCCCAACGCCACGGACATCTCCCCATTAAAg                                                  >  1:1334564/1‑149 (MQ=255)                                                                                                 tCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGAAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTg                                               >  2:91356/1‑149 (MQ=255)                                                                                                  cGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGc                                              <  2:1429659/149‑1 (MQ=255)                                                                                                       gAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGACGATg                                         >  2:1513258/1‑149 (MQ=255)                                                                                                        aaGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGGCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATgg                                        <  1:826853/149‑1 (MQ=255)                                                                                                                aaCGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGa                                <  1:963431/149‑1 (MQ=255)                                                                                                                  cGCTTGACGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATc                              <  1:446187/149‑1 (MQ=255)                                                                                                                       gACGATCTGCTGTCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAATCCACTGACATCTGCCCATTAAAGGTTCCGATGGCGTGCTGATCACCtt                         >  1:407272/1‑149 (MQ=255)                                                                                                                        aCGATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAATGTGCCGATGGCGTGCTGATCACCttt                        <  2:1481006/149‑1 (MQ=255)                                                                                                                          gATCTGCTGGCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGc                      >  1:1509468/1‑149 (MQ=255)                                                                                                                                  ggCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAAtgct              >  1:743402/1‑149 (MQ=255)                                                                                                                                  ggCAGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGAGGGCGTGCTGCTCACCTTTGCGAAAtgct              >  1:689413/1‑149 (MQ=255)                                                                                                                                     aGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgcc           >  2:746065/1‑149 (MQ=255)                                                                                                                                     aGAAATCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgcc           >  2:84945/1‑149 (MQ=255)                                                                                                                                       aaaTCGGACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgccgc         >  1:1377181/1‑149 (MQ=255)                                                                                                                                            ggACTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTAt    >  2:1173868/1‑149 (MQ=255)                                                                                                                                              aCTTTGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACCGTCATCTGCCGATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCTCCCTATTc  <  2:227095/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTC  >  NC_000913_3_hsa_pgi/3823842‑3824131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑