Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	1,933,245	G→A	Q454* (CAG→TAG)	edd ←	6‑phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	1,933,245	0	G	A	100.0%	211.8 / NA	70	Q454* (CAG→TAG)	edd	6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/31);  total (39/31)

CACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACA  >  NC_000913_3_hsa_pgi/1933103‑1933390                                                                                                                                               |                                                                                                                                                  cacCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTc                                                                                                                                             <  1:1096961/149‑1 (MQ=255)   ccGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCaa                                                                                                                                           >  1:1247676/1‑149 (MQ=255)          aataatACCCCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAaacacc                                                                                                                                    >  1:1000289/1‑147 (MQ=255)             aataCACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGc                                                                                                                                 <  2:543665/149‑1 (MQ=255)               accacCAAGTGGCGGCATGAGGTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTg                                                                                                                               <  2:331025/147‑1 (MQ=255)                              cATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCt                                                                                                                >  1:625165/1‑149 (MQ=255)                                tGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTgg                                                                                                              <  1:687040/149‑1 (MQ=255)                                    tttATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTtct                                                                                                          >  1:138545/1‑149 (MQ=255)                                       aTGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACTGACAACAACGACACAATCGCGGTCCAGCAAACCCGCGTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCGGGCGCTTCAATCACCTGGTTCTCaa                                                                                                       <  2:896595/149‑1 (MQ=255)                                        tGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGCCAACAACGACACAATCGCGGTCCAGCACACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAAc                                                                                                      >  2:1207977/1‑149 (MQ=255)                                               cTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACcggcacg                                                                                               >  2:85434/1‑149 (MQ=255)                                               cTGGCATTCCGTTCGCTTTTGGCCACTGATGACGGACAACAACGACACAATCGAGGTCAAGCAAACCCGATTCAAAGGCCGGCATAACGACATGCTAGCTTACAAAAACATCCCCTGGCGCTTCAATCACCTGGTTCTCAACcggcacc                                                                                               >  1:942938/1‑148 (MQ=255)                                                tGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACATCAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACcggcacgg                                                                                              <  2:963343/149‑1 (MQ=255)                                                  gCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGCCACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCCTAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGATTCCATCACCTGGTTCTCAACcggcaaggag                                                                                            >  2:869256/1‑147 (MQ=255)                                                   cATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCag                                                                                           >  1:1048683/1‑149 (MQ=255)                                                   cATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCag                                                                                           >  1:565508/1‑149 (MQ=255)                                                     ttCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAATCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCagag                                                                                         <  1:1328849/149‑1 (MQ=255)                                                          ttCGCTTTTGGCCCCTGATGACGGACAACAACGACACTATCTCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCTAAAACAACCGCTGGCGCTTCAATCTCCTGGTTCTCAACCGGCACGGCAGAGGtttt                                                                                    <  2:942938/149‑1 (MQ=255)                                                          ttCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGtttt                                                                                    >  2:1319046/1‑149 (MQ=255)                                                          ttCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGtttt                                                                                    <  2:679270/149‑1 (MQ=255)                                                          ttCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGAGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGtttt                                                                                    >  2:372401/1‑149 (MQ=255)                                                                tttGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAAc                                                                              <  1:471500/149‑1 (MQ=255)                                                                 ttGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAAcc                                                                             >  2:67326/1‑149 (MQ=255)                                                                   ggCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCGGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGc                                                                           >  2:1327641/1‑149 (MQ=255)                                                                    gCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCa                                                                          <  2:1000289/149‑1 (MQ=255)                                                                      cccTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACg                                                                        >  2:206933/1‑149 (MQ=255)                                                                      cccTGATGACGGACAACAACCACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCACAGGTTTTCATAACCCCACg                                                                        >  1:54533/1‑149 (MQ=255)                                                                       ccTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACgg                                                                       >  2:717250/1‑149 (MQ=255)                                                                       ccTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTACAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACATCCGCTGGCCCATCAAACACCTTGAACTCAACCGGCACGGCAGAGGTGTTCATAACCGCACgg                                                                       >  2:262440/1‑149 (MQ=255)                                                                        cTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAATGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGc                                                                      >  1:703677/1‑149 (MQ=255)                                                                          gatgaCGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGccc                                                                    >  1:1387908/1‑149 (MQ=255)                                                                            tgaCGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAg                                                                  <  1:457249/149‑1 (MQ=255)                                                                             gaCGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAgg                                                                 >  2:934661/1‑149 (MQ=255)                                                                                ggACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCAGGATAGGTTGCAAAAACAACCGGGGGGGCTGCAATCCCCTGGTTCACACCCGGCCCGGCAGCGGTTTTCTTAACCGCACGCCCCCGGTTa                                                              >  1:662073/1‑149 (MQ=255)                                                                                      aaacGACACAATCGCGGTCCAGCAAACCCGCTGCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCCATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCtt                                                        <  2:116367/148‑1 (MQ=255)                                                                                       aacGACACAATCGCGGTCCAGCAACCCCGCTTCAAAGGCCGGCATAACGCCATGCTAGCTTTCAAAAACAACAGCTGGCGCTTCAATCACCTGGTTCTAAACCCGCACGGCAGAGGTTGTCATAAACGGACGGCGCAGGTTACCGCTTa                                                       >  2:1187162/1‑149 (MQ=255)                                                                                       aacGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAACAACAACCGATGGGGCTTCAAACACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCCCGGCCCAGGATACCGCTTa                                                       >  2:751954/1‑149 (MQ=255)                                                                                       aacGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTa                                                       >  2:1162061/1‑149 (MQ=255)                                                                                        acGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTaa                                                      <  1:490809/149‑1 (MQ=255)                                                                                        acGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTaa                                                      <  2:169267/149‑1 (MQ=255)                                                                                         cGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAac                                                     >  1:587473/1‑149 (MQ=255)                                                                                               aaTCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCCCCTGGTTCTCAACCGCCACGCCAGAGCTTTTAATACCCCCACGGCCCAGGGTACCGCTTAACACgttt                                               >  2:1385373/1‑149 (MQ=255)                                                                                               aaTCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACtttt                                               <  2:1048683/149‑1 (MQ=255)                                                                                               aaTCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACtttt                                               >  2:53479/1‑149 (MQ=255)                                                                                               aaTCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACtttt                                               <  2:565508/149‑1 (MQ=255)                                                                                               aaTCGCGGGCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACtttt                                               <  1:806415/149‑1 (MQ=255)                                                                                                 tCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGt                                             <  2:138545/149‑1 (MQ=255)                                                                                                     ggTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCcca                                         <  2:1387908/149‑1 (MQ=255)                                                                                                       tCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTAcc                                                            >  2:235739/1‑128 (MQ=255)                                                                                                       tCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTAcc                                                            <  1:235739/128‑1 (MQ=255)                                                                                                         cacccaaCCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCATTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCATAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCat                                     <  1:374812/144‑1 (MQ=255)                                                                                                           gCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCatga                                   <  2:835007/149‑1 (MQ=255)                                                                                                                cccGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATgaga                              >  2:579627/1‑149 (MQ=255)                                                                                                                 ccGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATATCCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATgagag                             >  1:530017/1‑149 (MQ=255)                                                                                                                  cGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCCCCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCCCGGCCCAGGGTACCGCTTTACACTTTTGTCCCACCATGATgagaga                            >  2:491224/1‑149 (MQ=255)                                                                                                                  cGCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATACACGCACGGCCCAGGTTACCGCTTACCCCTTTTGTCCCACCATGAGgagaga                            >  2:329399/1‑149 (MQ=255)                                                                                                                   gCTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGaa                           >  2:481756/1‑149 (MQ=255)                                                                                                                   gCTTCAAAGGCCGACATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGCCCCGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTGAACACTTTTGTCCCACCATGATGAGAGaa                           >  2:801904/1‑149 (MQ=255)                                                                                                                    cTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGaaa                          <  2:1247676/149‑1 (MQ=255)                                                                                                                    cTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGaaa                          <  2:932352/149‑1 (MQ=255)                                                                                                                    cTTCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGaaa                          <  1:481756/149‑1 (MQ=255)                                                                                                                      tCAAAGGCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAgg                        >  1:168247/1‑149 (MQ=255)                                                                                                                        aaaGGCCGGCATAACGGCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGgtt                      <  2:1414974/149‑1 (MQ=255)                                                                                                                           ggCCGGCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAAGCGGCACGGCAGAGGTTTTCATGCCCGCACGGCCCCGGTTCCCGCATAACACTTTTGTCCCACCATGATGAGAGAAAGgttgtt                   >  2:1339289/1‑149 (MQ=255)                                                                                                                               ggCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCgaa               <  1:372401/149‑1 (MQ=255)                                                                                                                               ggCATAACGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCgaa               <  1:85434/149‑1 (MQ=255)                                                                                                                                    aaCGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCgaaggaag          <  1:1075102/149‑1 (MQ=255)                                                                                                                                     aCGTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGc         <  2:625165/149‑1 (MQ=255)                                                                                                                                       gTCATGCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATAACGTGTTACACACCCGGCACGGCAGAGGTTTTCATACCCGCCCGGCCCGGGTTACCGATGAACACTTTGTGCCCACCATGATGGTCTAAAGGTGGTTCGATGCAGGCGa       >  2:1319523/1‑149 (MQ=255)                                                                                                                                            gCTAGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATcaca  >  1:1175324/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                  CACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACA  >  NC_000913_3_hsa_pgi/1933103‑1933390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑