Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi526,7960CG22.7% 141.1 / 33.8 67A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base G (5/10);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGG  >  NC_000913_3_hsa_pgi/526649‑526938
                                                                                                                                                   |                                                                                                                                              
ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                               <  2:126535/149‑1 (MQ=255)
  gcgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGa                                                                                                                                             <  1:373745/149‑1 (MQ=255)
    gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGagt                                                                                                                                           <  2:256194/149‑3 (MQ=255)
      aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                         >  2:1225203/1‑149 (MQ=255)
      aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                         <  2:906918/149‑1 (MQ=255)
        tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                       <  1:474931/149‑1 (MQ=255)
        tGCGGGCTGACGGTGGAGCAACAGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                       <  1:514758/149‑1 (MQ=255)
         gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                      <  1:1165257/149‑1 (MQ=255)
             gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGCCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGATTATGATGAAt                                                                                                                                  >  2:34677/1‑149 (MQ=255)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                               <  1:570329/149‑1 (MQ=255)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATggg                                                                                                                               <  2:468160/149‑1 (MQ=255)
                 aCGGTGGAGCAACTGGTCAGCCAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgggg                                                                                                                              <  1:102047/149‑1 (MQ=11)
                    gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCaa                                                                                                                           >  1:119986/1‑149 (MQ=255)
                     tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                          >  2:362877/1‑149 (MQ=255)
                       gAGCAACTGGCCAGACAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCCTCTGTATCCCTTCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCACAACGGCGTGGAGCGCGTAAGAGGAAGACTGGGGCACCCa                                                                                                                        >  1:687534/1‑149 (MQ=17)
                         ccAACTGGCCAGACAGGGGGAGCCGGAATACACTCCGGCGCGAAAAGCTCATCTTTATCCCTGCGACCACCGGGGACGGCCGCTGGCGCTTATCACCGAAGACGGCAATACGGCGGGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                      <  2:633006/148‑1 (MQ=11)
                          cAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAACCTAATCTTTAACACTGAGACCACCGGGGACTGCCGCTGGCGCTTTTCAGCGAGGACGGCAATTCGTCGTGGCGCGGGGAGTATGAAGAATGGGGCAACCAGcg                                                                                                                     >  1:547254/1‑148 (MQ=11)
                             cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGAAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                                  <  1:1126188/149‑1 (MQ=255)
                              tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCTCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATAAGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                 <  1:915839/149‑1 (MQ=255)
                              tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                 <  1:188240/149‑1 (MQ=255)
                                 ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                              >  1:882053/1‑149 (MQ=255)
                                   aGACAGGTGGGGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                            >  1:1113331/1‑149 (MQ=255)
                                   aGACAGGTGGAGCCTGAATACCCACCGGCGCGAAACGCTCATCTTTATCACTGCGACCACCGTGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATCTGATGAATGGGGCAACCAGCTTAATgagca                                                                                                            <  1:965902/149‑3 (MQ=17)
                                   aGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                            <  1:965904/149‑1 (MQ=255)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGCCCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                      >  2:83334/1‑149 (MQ=11)
                                             aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                  <  2:1033459/149‑1 (MQ=255)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                 <  1:346662/149‑1 (MQ=255)
                                                cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                               <  1:125676/149‑2 (MQ=255)
                                                cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                               >  1:583413/1‑149 (MQ=255)
                                                 ggAATACACACCGGCGCGATTAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCTCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTATTGAGGAGAACCCGCATCAtg                                                                                              <  2:447407/149‑1 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGCCGGCAATACGGCGTGGAGCGCGGAATATGTTGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCAtgt                                                                                             >  1:111800/1‑149 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                             <  2:484549/149‑1 (MQ=255)
                                                  gAATAAACACCGGCGCGAAAAGCTCATCTTTAGCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                             <  1:826858/149‑4 (MQ=1)
                                                     tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACACGcatcacctgca                                                                                          >  2:428297/1‑143 (MQ=1)
                                                     tACACACCGGAGCGAAAAGCTCATCTTTATCACTGAGACCACCGTGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGAGGCGCGGGGAGGATGATGAATGGGGCAACCAGCGTAAGTAGGATAACCCGcatcacctgca                                                                                          >  1:639914/1‑143 (MQ=1)
                                                      acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                         <  2:600848/149‑1 (MQ=255)
                                                        acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACAGGGGACTGCCGCAGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCATGTGTATcc                                                                                       >  1:456945/1‑148 (MQ=255)
                                                            cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCATCGAATACGGCAATACGACGTCGAGCGATGATTAAGATGAATGTGGCAAACAGCTTATTGATGAGAACCCGCATCATGTGTAtaaaacg                                                                                   >  1:805052/1‑143 (MQ=255)
                                                            cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTAATCATCGAAGACGGCAATACGGCGTGGATCGCGGAAAATGTTGAATGGGGCAACCATCGTAATGAGGAGAACCCGCAACAAGTGTATCAGCCg                                                                                   >  1:271002/1‑149 (MQ=17)
                                                             ggCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGt                                                                                  >  1:26376/1‑149 (MQ=255)
                                                              gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCCGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTa                                                                                 <  1:1213746/149‑1 (MQ=255)
                                                                   aaaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGTGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                            <  2:567746/149‑1 (MQ=255)
                                                                     aaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTg                                                                          <  1:626049/149‑1 (MQ=255)
                                                                         tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCTCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                      <  1:996954/149‑1 (MQ=255)
                                                                         tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTGAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                      >  1:775408/1‑149 (MQ=255)
                                                                            tCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgc                                                                   <  1:403236/149‑1 (MQ=255)
                                                                                       gCGACCACCGGGTACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAGCGGCTGCCAGGGCAGCAGCatgat                                                        <  1:75934/149‑1 (MQ=255)
                                                                                          accaccGGGGACTGCCGCTGGCGGTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCATCCAGCTTTATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                     <  2:1132898/149‑1 (MQ=255)
                                                                                          accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGGGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                     >  1:619002/1‑149 (MQ=255)
                                                                                          accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                     >  1:198995/1‑149 (MQ=255)
                                                                                                  ggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAgg                                             <  2:28793/149‑1 (MQ=255)
                                                                                                   gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                            <  2:95570/149‑1 (MQ=255)
                                                                                                     cTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCt                                          <  2:1113331/149‑1 (MQ=255)
                                                                                                      tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCTAGGCCAGCAGCATGATGAGGATTCAGGGCTg                                         >  1:1229645/1‑149 (MQ=255)
                                                                                                        ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                       >  2:717426/1‑149 (MQ=255)
                                                                                                             ggCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACtata                                  >  1:1230509/1‑149 (MQ=255)
                                                                                                                     tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                          >  2:34440/1‑149 (MQ=255)
                                                                                                                      cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAcc                         >  1:458552/1‑149 (MQ=255)
                                                                                                                      cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAcc                         >  2:631367/1‑149 (MQ=255)
                                                                                                                      cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAGGATGAGGTATCAGGGCTGTACTATAACCGTCAcc                         >  1:494073/1‑149 (MQ=255)
                                                                                                                       aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                        >  2:1157226/1‑149 (MQ=255)
                                                                                                                        gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCTGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACAgg                       >  2:1075117/1‑149 (MQ=255)
                                                                                                                        gCGAAGACGGCAATACGGCCTGGAGCCCGGAATATGTTGAATGGGGCACCCAGCTTAATGAGGAGACCCCGCATCATGTGTTTCAGCCCTACCGTCTGCCAGCGCAGCAGCATGATGAGGAATCAGGTCTGTACTAAAACCGGCACAgg                       >  2:546325/1‑149 (MQ=17)
                                                                                                                          gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                     <  1:362877/149‑1 (MQ=255)
                                                                                                                                ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAAGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCCGGGCTGTACTATAACCGTCACCGGTACTACGa               >  1:503539/1‑149 (MQ=255)
                                                                                                                                  cAATACTGCGTGGATCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc             <  2:497878/149‑1 (MQ=255)
                                                                                                                                   aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATcc            >  1:731494/1‑149 (MQ=255)
                                                                                                                                             ggAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGAACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg  <  1:458835/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGG  >  NC_000913_3_hsa_pgi/526649‑526938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑