Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi526,8000AG21.3% 128.9 / 25.0 63E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base G (5/8);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_hsa_pgi/526655‑526948
                                                                                                                                                 |                                                                                                                                                    
aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                                   <  2:906918/149‑1 (MQ=255)
aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                                   >  2:1225203/1‑149 (MQ=255)
  tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                                 <  1:474931/149‑1 (MQ=255)
  tGCGGGCTGACGGTGGAGCAACAGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                                 <  1:514758/149‑1 (MQ=255)
   gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                                <  1:1165257/149‑1 (MQ=255)
       gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGCCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGATTATGATGAAt                                                                                                                                            >  2:34677/1‑149 (MQ=255)
          gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                         <  1:570329/149‑1 (MQ=255)
          gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATggg                                                                                                                                         <  2:468160/149‑1 (MQ=255)
           aCGGTGGAGCAACTGGTCAGCCAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgggg                                                                                                                                        <  1:102047/149‑1 (MQ=11)
              gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCaa                                                                                                                                     >  1:119986/1‑149 (MQ=255)
               tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                                    >  2:362877/1‑149 (MQ=255)
                 gAGCAACTGGCCAGACAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCCTCTGTATCCCTTCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCACAACGGCGTGGAGCGCGTAAGAGGAAGACTGGGGCACCCa                                                                                                                                  >  1:687534/1‑149 (MQ=17)
                   ccAACTGGCCAGACAGGGGGAGCCGGAATACACTCCGGCGCGAAAAGCTCATCTTTATCCCTGCGACCACCGGGGACGGCCGCTGGCGCTTATCACCGAAGACGGCAATACGGCGGGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                                <  2:633006/148‑1 (MQ=11)
                    cAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAACCTAATCTTTAACACTGAGACCACCGGGGACTGCCGCTGGCGCTTTTCAGCGAGGACGGCAATTCGTCGTGGCGCGGGGAGTATGAAGAATGGGGCAACCAGcg                                                                                                                               >  1:547254/1‑148 (MQ=11)
                       cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGAAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                                            <  1:1126188/149‑1 (MQ=255)
                        tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCTCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATAAGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                           <  1:915839/149‑1 (MQ=255)
                        tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                           <  1:188240/149‑1 (MQ=255)
                           ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                        >  1:882053/1‑149 (MQ=255)
                             aGACAGGTGGGGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                      >  1:1113331/1‑149 (MQ=255)
                             aGACAGGTGGAGCCTGAATACCCACCGGCGCGAAACGCTCATCTTTATCACTGCGACCACCGTGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATCTGATGAATGGGGCAACCAGCTTAATgagca                                                                                                                      <  1:965902/149‑3 (MQ=17)
                             aGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                      <  1:965904/149‑1 (MQ=255)
                                   gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGCCCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                                >  2:83334/1‑149 (MQ=11)
                                       aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                            <  2:1033459/149‑1 (MQ=255)
                                        gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                           <  1:346662/149‑1 (MQ=255)
                                          cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                                         <  1:125676/149‑2 (MQ=255)
                                          cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                         >  1:583413/1‑149 (MQ=255)
                                           ggAATACACACCGGCGCGATTAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCTCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTATTGAGGAGAACCCGCATCAtg                                                                                                        <  2:447407/149‑1 (MQ=255)
                                            gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGCCGGCAATACGGCGTGGAGCGCGGAATATGTTGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCAtgt                                                                                                       >  1:111800/1‑149 (MQ=255)
                                            gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                       <  2:484549/149‑1 (MQ=255)
                                            gAATAAACACCGGCGCGAAAAGCTCATCTTTAGCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                                       <  1:826858/149‑4 (MQ=1)
                                               tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACACGcatcacctgca                                                                                                    >  2:428297/1‑143 (MQ=1)
                                               tACACACCGGAGCGAAAAGCTCATCTTTATCACTGAGACCACCGTGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGAGGCGCGGGGAGGATGATGAATGGGGCAACCAGCGTAAGTAGGATAACCCGcatcacctgca                                                                                                    >  1:639914/1‑143 (MQ=1)
                                                acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                                   <  2:600848/149‑1 (MQ=255)
                                                  acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACAGGGGACTGCCGCAGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCATGTGTATcc                                                                                                 >  1:456945/1‑148 (MQ=255)
                                                      cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCATCGAATACGGCAATACGACGTCGAGCGATGATTAAGATGAATGTGGCAAACAGCTTATTGATGAGAACCCGCATCATGTGTAtaaaacg                                                                                             >  1:805052/1‑143 (MQ=255)
                                                      cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTAATCATCGAAGACGGCAATACGGCGTGGATCGCGGAAAATGTTGAATGGGGCAACCATCGTAATGAGGAGAACCCGCAACAAGTGTATCAGCCg                                                                                             >  1:271002/1‑149 (MQ=17)
                                                       ggCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGt                                                                                            >  1:26376/1‑149 (MQ=255)
                                                        gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCCGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTa                                                                                           <  1:1213746/149‑1 (MQ=255)
                                                             aaaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGTGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                                      <  2:567746/149‑1 (MQ=255)
                                                               aaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTg                                                                                    <  1:626049/149‑1 (MQ=255)
                                                                   tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCTCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                                <  1:996954/149‑1 (MQ=255)
                                                                   tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTGAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                                >  1:775408/1‑149 (MQ=255)
                                                                      tCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgc                                                                             <  1:403236/149‑1 (MQ=255)
                                                                                 gCGACCACCGGGTACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAGCGGCTGCCAGGGCAGCAGCatgat                                                                  <  1:75934/149‑1 (MQ=255)
                                                                                    accaccGGGGACTGCCGCTGGCGGTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCATCCAGCTTTATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                               <  2:1132898/149‑1 (MQ=255)
                                                                                    accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGGGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                               >  1:619002/1‑149 (MQ=255)
                                                                                    accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                               >  1:198995/1‑149 (MQ=255)
                                                                                            ggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAgg                                                       <  2:28793/149‑1 (MQ=255)
                                                                                             gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                      <  2:95570/149‑1 (MQ=255)
                                                                                               cTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCt                                                    <  2:1113331/149‑1 (MQ=255)
                                                                                                tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCTAGGCCAGCAGCATGATGAGGATTCAGGGCTg                                                   >  1:1229645/1‑149 (MQ=255)
                                                                                                  ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                                 >  2:717426/1‑149 (MQ=255)
                                                                                                       ggCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACtata                                            >  1:1230509/1‑149 (MQ=255)
                                                                                                               tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                                    >  2:34440/1‑149 (MQ=255)
                                                                                                                cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAcc                                   >  1:458552/1‑149 (MQ=255)
                                                                                                                cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAcc                                   >  2:631367/1‑149 (MQ=255)
                                                                                                                cAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAGGATGAGGTATCAGGGCTGTACTATAACCGTCAcc                                   >  1:494073/1‑149 (MQ=255)
                                                                                                                 aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                                  >  2:1157226/1‑149 (MQ=255)
                                                                                                                  gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCTGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACAgg                                 >  2:1075117/1‑149 (MQ=255)
                                                                                                                  gCGAAGACGGCAATACGGCCTGGAGCCCGGAATATGTTGAATGGGGCACCCAGCTTAATGAGGAGACCCCGCATCATGTGTTTCAGCCCTACCGTCTGCCAGCGCAGCAGCATGATGAGGAATCAGGTCTGTACTAAAACCGGCACAgg                                 >  2:546325/1‑149 (MQ=17)
                                                                                                                    gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                               <  1:362877/149‑1 (MQ=255)
                                                                                                                          ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAAGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCCGGGCTGTACTATAACCGTCACCGGTACTACGa                         >  1:503539/1‑149 (MQ=255)
                                                                                                                            cAATACTGCGTGGATCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                       <  2:497878/149‑1 (MQ=255)
                                                                                                                             aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATcc                      >  1:731494/1‑149 (MQ=255)
                                                                                                                                       ggAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGAACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg            <  1:458835/149‑1 (MQ=255)
                                                                                                                                               taaTATGATGATTGGGGCAACGAGCTTATTGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCGGCTAGGGCAGCAGCTTGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTatat    <  2:1229645/148‑1 (MQ=17)
                                                                                                                                                 atatGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTa  <  2:731494/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_hsa_pgi/526655‑526948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑