Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	3,397,306	C→A	E164* (GAG→TAG)	rng ←	ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	3,397,306	0	C	A	98.5%	209.3 / NA	66	E164* (GAG→TAG)	rng	ribonuclease G
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (27/38);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGC  >  NC_000913_3_hsa_pgi/3397161‑3397447                                                                                                                                                  |                                                                                                                                              tCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCtagtc                                                                                                                                            <  1:1081463/149‑1 (MQ=255) tCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCAATAACTTTGGTCGAGACGCGTTTCAGTTAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCtagtc                                                                                                                                            <  1:557295/149‑1 (MQ=255)  cGCCGTACAGCTGCTAACGGGTCTGCGGGCGTTTTTTACGCGCCATACCTTTGGTCCAGACGCGTTTCAGAGAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCGGCGGGACGGATGATAAACCCGCCCTGCtagtcg                                                                                                                                           <  1:720212/149‑1 (MQ=255)        aCAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATAc                                                                                                                                     >  2:55266/1‑149 (MQ=255)        aCAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATAc                                                                                                                                     <  1:473033/149‑1 (MQ=255)         cAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATAct                                                                                                                                    <  2:627357/149‑1 (MQ=255)             tGATTACGGGTCTGCTGGCTTTTTATACGCTCCATAACTTTGGTCCAGACTCGTTTCAGTTAAGCGTCATCGGATGACAGTTCAGCCTCGCCTACCCCTTCGGCTGCGTTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGc                                                                                                                                <  2:953137/149‑1 (MQ=255)             tGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGc                                                                                                                                <  1:992316/149‑1 (MQ=255)                  aCGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCa                                                                                                                           >  2:320222/1‑149 (MQ=255)                   cGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGAT‑AACCCGCCCTGCTAGTCGCAATACTCTGCGACCACt                                                                                                                         >  1:59684/1‑149 (MQ=255)                         tGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACtttttt                                                                                                                    <  1:55266/149‑1 (MQ=255)                            gggCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACTGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAg                                                                                                                 <  1:267449/149‑1 (MQ=255)                            gggCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAg                                                                                                                 >  1:611674/1‑149 (MQ=255)                             ggCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAgg                                                                                                                <  1:638937/149‑1 (MQ=255)                               cGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCg                                                                                                              >  1:990725/1‑149 (MQ=255)                               cGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGAAGCGGTACGGATGATAAACCCGCCCTGCTCGACGCAATACTCTGAGACCACTTTTTTCAcgcg                                                                                                              >  1:577577/1‑149 (MQ=255)                                gTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGATGCCAGTTCAGCCTCGCCAACCACTTCCGCTGCGGTACGGATGATAACCCCGCCCTGCTAGTCCCAATACTCTGCGACCACGTTTTTCAGGCGt                                                                                                             >  2:239520/1‑149 (MQ=255)                                gTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGt                                                                                                             >  1:82663/1‑149 (MQ=255)                                    tttACGCTCCATAACTTTGGTCCAGTCGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATTATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCAc                                                                                                         <  1:465459/149‑1 (MQ=255)                                    tttACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCAc                                                                                                         <  1:161763/149‑1 (MQ=255)                                           tCCATAACTTTGGTCCAGACGCGTTTGAGATAAGCGGCATCGGAGGCCAGATCAGCCTCGCCAACCCCTTCCGCTTAGGTTCGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGTCCACTTTTTTCAGGGGTTCACGTTCTGa                                                                                                  <  1:827262/149‑1 (MQ=255)                                           tCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGa                                                                                                  <  2:366726/149‑1 (MQ=255)                                           tCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGa                                                                                                  >  1:975938/1‑149 (MQ=255)                                             cATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGAtt                                                                                                <  2:868651/149‑1 (MQ=255)                                             cATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACACCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGAtt                                                                                                <  1:981565/149‑1 (MQ=255)                                                   tttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCttt                                                                                          <  2:170211/149‑1 (MQ=255)                                                        tCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATa                                                                                     <  2:161590/149‑1 (MQ=255)                                                         gcAGACGGGGTTCAGTTTAGCGGCTTCGGAGTGCAGTTGTGGCTGGCCCACGCGTTACGCTGCGGTACGGATGATAACGCCGCCCTGCTAGGCTCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATAc                                                                                    <  2:850884/148‑1 (MQ=255)                                                          cAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACATTTTTCAGGCGTTCCCGATCAGCTTAGCGTTccaatcg                                                                                   >  1:6957/1‑143 (MQ=255)                                                             aCGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTg                                                                                <  1:615164/149‑1 (MQ=255)                                                                 gTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGAT‑AACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGaaa                                                                           <  2:59684/149‑1 (MQ=255)                                                                   ttCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAAc                                                                          <  1:1230956/149‑1 (MQ=255)                                                                   ttCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAAc                                                                          <  1:984135/149‑1 (MQ=255)                                                                       gATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGCCCACTTTTTTCAGGCGTTCACGTTCGGATTCGCTTTCAATACGTGGGGAAACCCCa                                                                      >  1:1071633/1‑149 (MQ=255)                                                                       gATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCa                                                                      >  2:748216/1‑149 (MQ=255)                                                                          aaGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACg                                                                   <  1:767869/149‑1 (MQ=255)                                                                           aGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCGATACGTTGGGAAACCCCAACGt                                                                  <  1:320222/149‑1 (MQ=255)                                                                           aGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGt                                                                  <  2:611674/149‑1 (MQ=255)                                                                            gCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTTACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTg                                                                 >  2:819576/1‑149 (MQ=255)                                                                              ggCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTgag                                                               >  2:125293/1‑149 (MQ=255)                                                                               gCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTGCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGTCCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCATCGTgaga                                                              <  1:433625/149‑1 (MQ=255)                                                                               gCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTgaga                                                              <  1:1106690/149‑1 (MQ=255)                                                                                 aTCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGGTTTCCCAACGtattgaa                                                            <  2:747761/149‑7 (MQ=255)                                                                                 aTCGGAGGCCAGTGCAGCCTCGCCAACCCCATCCGCTGCGGTACGCATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTATCAGGCGTTCACGTTCTGAATCGCTTTCAATACGTGGGGGTTTCCCAACGtattgaa                                                            <  1:638313/149‑7 (MQ=255)                                                                                    ggAGGCCAGTTCAGCGTCGCCTACCCCTTCCGCTGCGGAACGGATGATAATCCCGCCCTGCTAGTCGCAATTCTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCACTACGTTGGGAAACCCCAACGTGAGAAGccc                                                         <  1:769122/149‑1 (MQ=255)                                                                                     gAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGcccc                                                        >  2:993006/1‑149 (MQ=255)                                                                                      aGGCAAGTTCAGCCTCGCCAACCCCTTCGGATGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTTCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCt                                                       <  2:301392/149‑1 (MQ=255)                                                                                         ccAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTTACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGc                                                    <  1:819576/149‑1 (MQ=255)                                                                                          cAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTAAATACGTTGGGCAACCCCAACGTGAGAAGCCCCTGGCa                                                   >  1:81279/1‑149 (MQ=255)                                                                                                 gCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAacac                                            >  2:283705/1‑149 (MQ=255)                                                                                                  ccTCGCCAACCCCTTCCGCTGCGGTACGGATGATAACCCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACAcc                                           >  2:737128/1‑149 (MQ=255)                                                                                                    tCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAg                                         >  2:296929/1‑149 (MQ=255)                                                                                                     cgcgAACCCCTTCCGCTGCGGTCCGGATGATAAACGCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGa                                        <  2:1193406/149‑1 (MQ=255)                                                                                                     cGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGa                                        <  2:7813/149‑1 (MQ=255)                                                                                                            cccTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTAAATACGTTGGGAAACCCCAACGTGAGAAGACCCTGGCATAAACACCAGATAGCgag                                 >  2:1117894/1‑149 (MQ=255)                                                                                                              cTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGaa                               <  2:564600/149‑1 (MQ=255)                                                                                                                  cGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGa                           >  2:585376/1‑149 (MQ=255)                                                                                                                  cGCTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGa                           >  1:246810/1‑149 (MQ=255)                                                                                                                    cTGCGGTACGGATGATAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGACACCCCAACGTGAGAAGCCCCGGGCATAAACACCAGATAGCGAGAAGGGAGc                         >  1:555077/1‑149 (MQ=255)                                                                                                                              gatgatAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTGCTGATTCGCTTTCAATACGTTGGGAAACCCCACAGTGAGAAGCCCCTGGCATAAACACCAGAAAGCGAGAAGGGAGTGTGATATCgg               >  2:978332/1‑149 (MQ=255)                                                                                                                              gatgatAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTAAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCgg               >  2:337953/1‑149 (MQ=255)                                                                                                                               atgatAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCggt              <  2:975938/149‑1 (MQ=255)                                                                                                                                tgatAAACCCGCCCTGCTAGTCGCAATACTCTGCTACCACTTTTTTCAGGCGGTCACGTTCTGATTCGCTTGCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCggtg             <  2:195612/149‑1 (MQ=255)                                                                                                                                tgatAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCggtg             <  2:781603/149‑1 (MQ=255)                                                                                                                                  atAACCCCGCCCTGCTAGTCGCAATACTCTGCGACCCCTTATTTCAGGCGTTCACGTGCGGATTCGCTTTCAATACGTTGGGCACCCCCAACGTGAGAAGCCCCGGGCATACTCACAAGAGAGCGAGAAGGGCGCGGGAGATCggtggt           >  1:310902/1‑149 (MQ=255)                                                                                                                                  atAAACCCGCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATCCGTTGGGAACCCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCggtggt           >  2:829886/1‑149 (MQ=255)                                                                                                                                   tAAACCCGCCCTGCTAGTGGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGTTAGCGAGAAGGGAGCGTGATATCGGTGGTc          <  1:637347/149‑1 (MQ=255)                                                                                                                                          gCCCTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGgcgcg   >  2:2012/1‑149 (MQ=255)                                                                                                                                           cccTGCTAGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGgcgcgc  <  1:585376/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              TCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGC  >  NC_000913_3_hsa_pgi/3397161‑3397447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑