Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi526,7920AC26.6% 171.9 / 72.1 129S1178R (AGC→CGC) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (53/41);  new base C (12/22);  total (65/64)
Fisher's exact test for biased strand distribution p-value = 4.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

GTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCG  >  NC_000913_3_hsa_pgi/526644‑526940
                                                                                                                                                    |                                                                                                                                                    
ggggCTTGCGCAGTGCGGGCTGACGGGGGAGCAACTGGCCAGACAGGCGGAGCCGGACGACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGa                                                                                                                                                      <  1:1054165/147‑1 (MQ=255)
gTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGa                                                                                                                                                      <  1:617051/149‑1 (MQ=255)
  ggCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGgcgc                                                                                                                                                    <  1:247273/149‑1 (MQ=255)
   gCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATGCGGCGTGGcgcg                                                                                                                                                   >  1:1338855/1‑149 (MQ=255)
   gCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACACGCGCGAAAAGCTCATCGTTATCACTGCGACCACCGGGGACAGCCCCTGGCTCTTAACAGCGAAGACGGCAATACGGCGAGgcgcc                                                                                                                                                   >  1:460994/1‑148 (MQ=14)
    cTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGAGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGCATGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcgg                                                                                                                                                  >  1:1166698/1‑148 (MQ=255)
     ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                                                 >  1:843243/1‑147 (MQ=255)
     tggcgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                                                 <  2:1111005/147‑3 (MQ=255)
     ctGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGCGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                                                 <  1:1056328/148‑3 (MQ=255)
        cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGAGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGATCGAGGaa                                                                                                                                              >  1:464090/1‑149 (MQ=255)
         gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAat                                                                                                                                             >  1:24574/1‑149 (MQ=255)
           aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGTCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                           <  2:1566080/149‑1 (MQ=11)
                  gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGTAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGACGTCGGCAATACGGATTGTAGCGCGGAATATGATGAAt                                                                                                                                    >  1:920159/1‑149 (MQ=17)
                  gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGGCGCTTATCATCGAAGACGGCAATACGTCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                    <  2:1189845/149‑1 (MQ=11)
                    tgtcGGTTGAGCAACTGGCCAGACAGGTGGAGCCGGATTACACACCGGCGCGAATATCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTTGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                  <  2:669838/146‑1 (MQ=11)
                    tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGTGCGGGGAGTATGATGAATgg                                                                                                                                  <  1:747977/149‑1 (MQ=255)
                     taCGGTGGAGCAACTGGCCAGACTGGTGGAGCCGGAATACACACCTGCGCGATTAGCTCATGATTATCACTGCGACCACCGGGGACTGCCGCTGGCGATTATCAGCGAAGACGTCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                 <  2:1052234/148‑1 (MQ=14)
                     gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGACTACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTATCAGCGAAGACGGCAATACGGCGGGGCGCGGGGAGTATGATGAATggg                                                                                                                                 <  1:1031517/149‑1 (MQ=11)
                     gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                 <  1:256336/149‑1 (MQ=255)
                      aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACCCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                <  1:1433767/149‑1 (MQ=255)
                      aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgggg                                                                                                                                >  2:195336/1‑149 (MQ=255)
                           ggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAcc                                                                                                                           >  2:558700/1‑149 (MQ=255)
                           ggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCACCGAAGACGGAAATACCGCGTGGCGCGGGGAGTATGATGAATCGTGCAAcc                                                                                                                           >  1:1525005/1‑149 (MQ=11)
                            gAGCAACTGGCCAGACAGGTGGCGCCGGAATACACACAGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCATATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGATTGGGGCAACCa                                                                                                                          >  2:1235779/1‑149 (MQ=255)
                              gCAACTGGGCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                        <  1:504761/149‑1 (MQ=255)
                                 aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTa                                                                                                                     >  1:598305/1‑149 (MQ=255)
                                 aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                     <  1:1463980/149‑1 (MQ=255)
                                 aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                     >  1:953575/1‑149 (MQ=255)
                                     gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                 >  1:974219/1‑149 (MQ=255)
                                      ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCACCCAGCTTAATgag                                                                                                                <  1:147230/149‑1 (MQ=255)
                                      ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                <  2:986937/149‑1 (MQ=255)
                                      ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                <  2:408301/149‑1 (MQ=255)
                                       cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                               <  1:158273/149‑1 (MQ=255)
                                       cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                               <  1:92792/149‑1 (MQ=255)
                                       cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                               <  1:152681/149‑1 (MQ=255)
                                       cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                               >  1:312327/1‑149 (MQ=255)
                                        cgACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                              <  2:787713/148‑1 (MQ=255)
                                        aGACTGGTGGAGCCGGATTACTCACCGGCGCGAAATGCTCATCTTTATCACTGCTAACACCGGGGACTGCCTCTTGCTCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAATATGATGAATGGGGGAACCAGCTTAAtgatga                                                                                                              <  2:1337455/149‑1 (MQ=14)
                                        aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                              <  2:739834/149‑1 (MQ=255)
                                        aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                              <  1:578798/149‑1 (MQ=255)
                                         gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgaggat                                                                                                             >  1:765615/1‑148 (MQ=255)
                                          aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTTCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCCGGCGTGGCGCGGGGAGTAAGATGATTGGGGAACCCAGCTGAAGGAGgaga                                                                                                            >  1:412125/1‑149 (MQ=11)
                                            aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGTATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                          >  2:988801/1‑149 (MQ=255)
                                            aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGAGCGGAATATGATGAATGGGGCAAACAGCTTAATGAGGAGAAc                                                                                                          >  1:944418/1‑149 (MQ=255)
                                            aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAGACGGCAATGCGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                          >  2:1352205/1‑149 (MQ=255)
                                               tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                       >  1:1000127/1‑149 (MQ=255)
                                                   gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGCCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCACCTTAATGAGGAGCACCCGcatc                                                                                                   >  2:1039984/1‑149 (MQ=11)
                                                    ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcc                                                                                                  >  1:1351722/1‑148 (MQ=255)
                                                       gAATACACACCGGCGCGAAAAGCTCATCTTTATCCCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCCGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                               >  2:1277429/1‑149 (MQ=255)
                                                       gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                               >  2:1325615/1‑146 (MQ=255)
                                                       gAATACACACCGGCGCGAAAAGCTCATCTTAATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                               <  1:940447/149‑1 (MQ=255)
                                                          tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTa                                                                                            >  1:661737/1‑149 (MQ=255)
                                                           acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCTGGGACTGCCGCTGGCGCTTATCACCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                           <  1:190932/149‑8 (MQ=11)
                                                           acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                           <  1:810771/149‑8 (MQ=11)
                                                            cacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                          >  2:507650/1‑149 (MQ=255)
                                                             acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                         >  2:437417/1‑149 (MQ=255)
                                                                 cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCg                                                                                     >  2:419618/1‑149 (MQ=255)
                                                                   gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTa                                                                                   >  1:1161829/1‑149 (MQ=255)
                                                                          aaGCTCATCTTTATCACTGCGGCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGAAAATCGGCGTGGAGCGCGGAATATGAAGAATGGGGCAACCAGCTTAATGAGGAGAACCCGGAACATGGGTATCAGCCGGACCGACTg                                                                            >  1:429299/1‑149 (MQ=18)
                                                                          aaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAATATGATGAATGGGGCAACCAGCGTAATGGGGCGCGCCCGCATCCTGGGGAGCAGCCGTGCCGTcag                                                                            >  2:1011541/1‑147 (MQ=14)
                                                                            gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGcc                                                                          >  1:1104777/1‑149 (MQ=255)
                                                                              tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATCATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGAGTAGCAGCCGTACCGTCTGCGAg                                                                        >  2:517828/1‑149 (MQ=255)
                                                                                aTCTTTATCACTGCGCCCACCGGCGACTGCCGCTGGCGCATTTAAGCGAAGACGGCAATACGGCGTGGAGAGCGGATTATGAAGAATGGGGCAACCAGCTTAAAGAGGAGAACCCGCATCATTTGTATCAGACTTACCGACAGCCAggg                                                                      >  2:137152/1‑149 (MQ=17)
                                                                                aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                      >  2:1195898/1‑149 (MQ=255)
                                                                                aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGAGGAATATGATGAATGGGGCAAACAGCTTAATGAGGAGAACCCGCATCATGTGTATAAGCCGTACAGTCTGAAAggg                                                                      >  1:974099/1‑149 (MQ=17)
                                                                                      aTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                                <  1:1494554/149‑1 (MQ=255)
                                                                                          cTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCCACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCATGgcagcacgttg                                                            >  1:42659/1‑144 (MQ=255)
                                                                                          cTGCGACCACCGGGGACTGCCGCTGCCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAAGATGATGAATGGGGCAACCAGCTTAATGAGGCGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatg                                                            >  2:380723/1‑149 (MQ=255)
                                                                                           tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                           >  1:873244/1‑149 (MQ=255)
                                                                                           tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                           >  2:817836/1‑149 (MQ=255)
                                                                                           tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                           >  2:565371/1‑149 (MQ=255)
                                                                                            gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                          <  2:616974/149‑1 (MQ=255)
                                                                                               accaccGGCGACTGCCGCTGGCGATTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGACCACGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatgcg                                                       <  2:916189/149‑3 (MQ=255)
                                                                                                 caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAggt                                                     >  1:268964/1‑148 (MQ=255)
                                                                                                 caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCGTCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                          >  2:473930/1‑144 (MQ=255)
                                                                                                 caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                     >  1:684119/1‑149 (MQ=255)
                                                                                                    cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAta                                                  >  1:325086/1‑148 (MQ=255)
                                                                                                           tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAGGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                           >  1:1311363/1‑149 (MQ=255)
                                                                                                            gCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGt                                          <  1:548266/149‑1 (MQ=255)
                                                                                                             cctcTGCCGCTTATCAGCGAATACGGCAATACGGCGTGGAGCGCGGAAAATTATGAATGGCGCAACCAGCTTAATGAGGTGAACCCGCATCATGTGTATCAGCAGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                         <  1:865228/146‑1 (MQ=17)
                                                                                                                   gcgcTTATCAGCGAAGACGGCAATACGGCGTTGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                   <  2:1359667/149‑1 (MQ=255)
                                                                                                                     gcTTATCAGCGAAGCCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTACTGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCTGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                 <  1:250395/149‑1 (MQ=255)
                                                                                                                     gcTTATCAGCGAAGACGGCAAGACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                 <  1:1011541/149‑1 (MQ=255)
                                                                                                                          tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCCACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTccc                            >  2:1115702/1‑147 (MQ=255)
                                                                                                                          tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                      >  2:203575/1‑107 (MQ=255)
                                                                                                                          tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                      <  1:203575/107‑1 (MQ=255)
                                                                                                                            aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTTTAACCGTCAGCg                          >  2:378987/1‑149 (MQ=255)
                                                                                                                            aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                          <  2:248135/149‑1 (MQ=255)
                                                                                                                             gCGAAGACGGAAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCTGGGAAGCAGCATTATGAGGAATCTGGGCTGTACTATAACCGTCACCgg                         <  1:1089409/149‑1 (MQ=17)
                                                                                                                               gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                       <  2:24574/149‑1 (MQ=255)
                                                                                                                                  gACGGCAATACGGCGTGGAGCGCGTAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                    >  1:992985/1‑149 (MQ=255)
                                                                                                                                  gACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCCACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAGGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                    >  2:173012/1‑149 (MQ=255)
                                                                                                                                  gACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGTGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                    >  2:189554/1‑149 (MQ=255)
                                                                                                                                      gCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGAt                <  2:1000127/149‑1 (MQ=255)
                                                                                                                                       cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTCCCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc               >  2:930993/1‑149 (MQ=255)
                                                                                                                                        aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATcc              >  1:123428/1‑149 (MQ=255)
                                                                                                                                          tACGGCGTGGAGCGCGTAATATGATGTATGGGGCATCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt            <  2:1068654/149‑1 (MQ=255)
                                                                                                                                             ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCAGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGttcc         >  1:1412800/1‑147 (MQ=255)
                                                                                                                                                  ggAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg    <  2:661737/149‑1 (MQ=255)
                                                                                                                                                    aGCGCGTAATATGATGAATGGGGAAACCAGTTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGGATGATGAGGAATCAGGGCTGTACTTTAACCGTCACCGGTACTACGATCCGTTGCAGGGGCg  <  2:1140980/149‑1 (MQ=17)
                                                                                                                                                    |                                                                                                                                                    
GTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCG  >  NC_000913_3_hsa_pgi/526644‑526940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑