Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi526,8000AG24.2% 218.1 / 73.3 129E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (52/45);  new base G (11/20);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 9.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 135 total aligned reads displayed.

CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATT  >  NC_000913_3_hsa_pgi/526652‑526947
                                                                                                                                                    |                                                                                                                                                   
 gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAat                                                                                                                                                    >  1:24574/1‑149 (MQ=255)
  cAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACCGGAGGCGCCGGAAAACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCCAAGACGGCAATACGGCGTGGCGCGGGGAgta                                                                                                                                                   <  1:1042768/149‑1 (MQ=11)
   aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGTCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                                  <  2:1566080/149‑1 (MQ=11)
   aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                                  <  1:621194/149‑1 (MQ=255)
          gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGGCGCTTATCATCGAAGACGGCAATACGTCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                           <  2:1189845/149‑1 (MQ=11)
            tgtcGGTTGAGCAACTGGCCAGACAGGTGGAGCCGGATTACACACCGGCGCGAATATCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTTGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                         <  2:669838/146‑1 (MQ=11)
            tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGTGCGGGGAGTATGATGAATgg                                                                                                                                         <  1:747977/149‑1 (MQ=255)
             taCGGTGGAGCAACTGGCCAGACTGGTGGAGCCGGAATACACACCTGCGCGATTAGCTCATGATTATCACTGCGACCACCGGGGACTGCCGCTGGCGATTATCAGCGAAGACGTCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                        <  2:1052234/148‑1 (MQ=14)
             gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                        <  1:256336/149‑1 (MQ=255)
             gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                        <  1:1460357/149‑1 (MQ=255)
              aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACCCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                       <  1:1433767/149‑1 (MQ=255)
              aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgggg                                                                                                                                       >  2:195336/1‑149 (MQ=255)
                  tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                   <  2:470103/149‑1 (MQ=255)
                   ggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAcc                                                                                                                                  >  2:558700/1‑149 (MQ=255)
                   ggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCACCGAAGACGGAAATACCGCGTGGCGCGGGGAGTATGATGAATCGTGCAAcc                                                                                                                                  >  1:1525005/1‑149 (MQ=11)
                    gAGCAACTGGCCAGACAGGTGGCGCCGGAATACACACAGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCATATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGATTGGGGCAACCa                                                                                                                                 >  2:1235779/1‑149 (MQ=255)
                      gCAACTGGGCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                               <  1:504761/149‑1 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                               >  1:1502761/1‑149 (MQ=255)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAAGATGATGAATCGGGCAACCAGc                                                                                                                               >  1:956493/1‑149 (MQ=255)
                         aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTa                                                                                                                            >  1:598305/1‑149 (MQ=255)
                         aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                            <  1:1463980/149‑1 (MQ=255)
                         aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                            >  1:953575/1‑149 (MQ=255)
                           tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATGCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                          <  2:1338855/149‑1 (MQ=255)
                            ggCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATg                                                                                                                         <  2:1297780/149‑1 (MQ=255)
                             gCCAGACAGGTGGCGCCGGAATACACACCGGCGCGAAGAGCTCATCTTTATCCCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAGGACGGCAATACGGCGTGGAGCGCGGAAGATGATGAAGGGGGCAACCAGCTTTATga                                                                                                                        >  2:1440032/1‑149 (MQ=17)
                             gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATga                                                                                                                        >  1:1542264/1‑149 (MQ=255)
                             gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                        >  2:548266/1‑149 (MQ=255)
                             gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGAAGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                        >  2:816435/1‑149 (MQ=255)
                              ccAGACAGGTGGATCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                       <  1:1368248/149‑1 (MQ=255)
                              ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                       <  2:986937/149‑1 (MQ=255)
                              ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                       <  2:408301/149‑1 (MQ=255)
                               cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgcgg                                                                                                                      >  2:1192640/1‑149 (MQ=11)
                               cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                      <  1:158273/149‑1 (MQ=255)
                               cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                      <  1:92792/149‑1 (MQ=255)
                               cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                      >  1:312327/1‑149 (MQ=255)
                                cgACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                     <  2:787713/148‑1 (MQ=255)
                                aGACTGGTGGAGCCGGATTACTCACCGGCGCGAAATGCTCATCTTTATCACTGCTAACACCGGGGACTGCCTCTTGCTCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAATATGATGAATGGGGGAACCAGCTTAAtgatga                                                                                                                     <  2:1337455/149‑1 (MQ=14)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTGATCAGCGATGACGGCAATACGGCGGGGAGCGCGGAAGATGATGAATGGGGCACCCAGCTTAAAgagga                                                                                                                     >  2:1021552/1‑149 (MQ=14)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                     <  1:579161/149‑1 (MQ=255)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                     <  2:739834/149‑1 (MQ=255)
                                aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                     <  1:874676/149‑1 (MQ=255)
                                 gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgaggat                                                                                                                    >  1:765615/1‑148 (MQ=255)
                                    aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGTATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                 >  2:988801/1‑149 (MQ=255)
                                    aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAGACGGCAATGCGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                 >  2:1352205/1‑149 (MQ=255)
                                       tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                              >  1:1000127/1‑149 (MQ=255)
                                         gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                            <  1:936186/149‑1 (MQ=255)
                                          aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGAAATACGGCGTGGAGCGCGGAATTTGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                           <  1:448981/149‑1 (MQ=255)
                                           gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGCCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCACCTTAATGAGGAGCACCCGcatc                                                                                                          >  2:1039984/1‑149 (MQ=11)
                                            ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcc                                                                                                         >  1:1351722/1‑148 (MQ=255)
                                               gAATACACACCGGCGCGAAAAGCTCATCTTTATCCCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCCGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                      >  2:1277429/1‑149 (MQ=255)
                                               gAATACACACCGGCGCGAAAAGCTCATCTTAATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                      <  1:940447/149‑1 (MQ=255)
                                                aaTACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgtg                                                                                                     <  2:177611/149‑1 (MQ=255)
                                                   acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                                  <  1:810771/149‑8 (MQ=11)
                                                    cacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                                 >  2:507650/1‑149 (MQ=255)
                                                     acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                                <  1:108360/149‑1 (MQ=255)
                                                         cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCg                                                                                            >  2:419618/1‑149 (MQ=255)
                                                           gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTa                                                                                          >  1:1161829/1‑149 (MQ=255)
                                                                  aaGCTCATCTTTATCACTGCGGCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGAAAATCGGCGTGGAGCGCGGAATATGAAGAATGGGGCAACCAGCTTAATGAGGAGAACCCGGAACATGGGTATCAGCCGGACCGACTg                                                                                   >  1:429299/1‑149 (MQ=18)
                                                                        aTCTTTATCACTGCGCCCACCGGCGACTGCCGCTGGCGCATTTAAGCGAAGACGGCAATACGGCGTGGAGAGCGGATTATGAAGAATGGGGCAACCAGCTTAAAGAGGAGAACCCGCATCATTTGTATCAGACTTACCGACAGCCAggg                                                                             >  2:137152/1‑149 (MQ=17)
                                                                        aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGAGGAATATGATGAATGGGGCAAACAGCTTAATGAGGAGAACCCGCATCATGTGTATAAGCCGTACAGTCTGAAAggg                                                                             >  1:974099/1‑149 (MQ=17)
                                                                              aTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                                       <  1:1494554/149‑1 (MQ=255)
                                                                                cACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcagca                                                                     <  2:417734/149‑1 (MQ=255)
                                                                                 aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCat                                                                    >  1:991487/1‑149 (MQ=255)
                                                                                  cTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCCACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCATGgcagcacgttg                                                                   >  1:42659/1‑144 (MQ=255)
                                                                                   tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                                  >  1:873244/1‑149 (MQ=255)
                                                                                   tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                                  >  2:565371/1‑149 (MQ=255)
                                                                                    gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                                 <  2:616974/149‑1 (MQ=255)
                                                                                       accaccGGCGACTGCCGCTGGCGATTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGACCACGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatgcg                                                              <  2:916189/149‑3 (MQ=255)
                                                                                         caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAggt                                                            >  1:268964/1‑148 (MQ=255)
                                                                                         caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCGTCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                                 >  2:473930/1‑144 (MQ=255)
                                                                                         caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCGTCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                                 <  1:473930/144‑1 (MQ=255)
                                                                                         caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                            >  1:684119/1‑149 (MQ=255)
                                                                                            cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAta                                                         >  1:325086/1‑148 (MQ=255)
                                                                                                gACTGTCGCTGGCGCTTATCAGCGAAGACGGCAATACCGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAGCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                     <  1:397261/149‑1 (MQ=255)
                                                                                                   tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAGGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                                  >  1:1311363/1‑149 (MQ=255)
                                                                                                    gCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGt                                                 <  1:548266/149‑1 (MQ=255)
                                                                                                     cctcTGCCGCTTATCAGCGAATACGGCAATACGGCGTGGAGCGCGGAAAATTATGAATGGCGCAACCAGCTTAATGAGGTGAACCCGCATCATGTGTATCAGCAGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                                <  1:865228/146‑1 (MQ=17)
                                                                                                     ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGTCAGCAGCATGATGAGGAATCAGGGCTGTa                                                <  1:1163726/149‑1 (MQ=255)
                                                                                                             gcTTATCAGCGAAGCCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTACTGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCTGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                        <  1:250395/149‑1 (MQ=255)
                                                                                                             gcTTATCAGCGAAGACGGCAAGACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                        <  1:1011541/149‑1 (MQ=255)
                                                                                                               ttATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGt                                      >  2:282786/1‑149 (MQ=255)
                                                                                                                  tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCCACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTccc                                   >  2:1115702/1‑147 (MQ=255)
                                                                                                                  tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                             <  1:203575/107‑1 (MQ=255)
                                                                                                                    aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCACCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTTTAACCGTCAGCg                                 >  2:378987/1‑149 (MQ=255)
                                                                                                                    aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                                 <  2:248135/149‑1 (MQ=255)
                                                                                                                     gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                                <  1:23493/149‑1 (MQ=255)
                                                                                                                     gCGAAGACGGAAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCTGGGAAGCAGCATTATGAGGAATCTGGGCTGTACTATAACCGTCACCgg                                <  1:1089409/149‑1 (MQ=17)
                                                                                                                       gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                              <  2:24574/149‑1 (MQ=255)
                                                                                                                         aGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGATGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtact                            <  1:816435/149‑1 (MQ=255)
                                                                                                                          gACGGCAATACGGCGTGGAGCGCGTAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                           >  1:992985/1‑149 (MQ=255)
                                                                                                                          gACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGTGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                           >  2:189554/1‑149 (MQ=255)
                                                                                                                              gCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGAt                       <  2:1000127/149‑1 (MQ=255)
                                                                                                                               cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTCCCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                      >  2:930993/1‑149 (MQ=255)
                                                                                                                                aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATcc                     >  1:123428/1‑149 (MQ=255)
                                                                                                                                  tACGGCGTGGAGCGCGTAATATGATGTATGGGGCATCCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt                   <  2:1068654/149‑1 (MQ=255)
                                                                                                                                          ggAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg           <  2:661737/149‑1 (MQ=255)
                                                                                                                                            aGCGCGTAATATGATGAATGGGGAAACCAGTTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGGATGATGAGGAATCAGGGCTGTACTTTAACCGTCACCGGTACTACGATCCGTTGCAGGGGCg         <  2:1140980/149‑1 (MQ=17)
                                                                                                                                             gcgcGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCgg        <  1:1021552/149‑1 (MQ=255)
                                                                                                                                                 ggAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCATCAGCATGATGAGGAATCCGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGtaga    >  2:823139/1‑147 (MQ=255)
                                                                                                                                                   aaTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGAATAGGAATCAGGGCTGTAATATAACCGTCACCGGTACTAAGATCCGTTGCAGGGGCGTTAAAtt  >  2:37619/1‑149 (MQ=17)
                                                                                                                                                    |                                                                                                                                                   
CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATT  >  NC_000913_3_hsa_pgi/526652‑526947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑