Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi1,019,7340GT20.6% 114.1 / 15.0 68P107Q (CCA→CAA) ompAouter membrane protein A (3a;II*;G;d)
Reads supporting (aligned to +/- strand):  ref base G (24/30);  new base T (11/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 3.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACC  >  NC_000913_3_hsa_pgi/1019586‑1019879
                                                                                                                                                    |                                                                                                                                                  
gATTTCAGGAGTGATCGCGTAATCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCATGACGTCAGTGATTg                                                                                                                                                    >  1:1234731/1‑149 (MQ=255)
 aTTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTgg                                                                                                                                                   <  2:509674/149‑1 (MQ=255)
       ggtgTGATCGCGTACTCAACACCGCCAGCGTACACCGCAGAAACGCCGGTGTCGTGGTTTTTACCATATACGGTGGATTTAGCGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAAcc                                                                                                                                             <  1:1193521/146‑1 (MQ=255)
           tGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTTGGTAACCCAGt                                                                                                                                         >  1:517121/1‑149 (MQ=255)
            gATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGCATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTTGATAACCCatgt                                                                                                                                        >  2:741187/1‑146 (MQ=255)
              tCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTa                                                                                                                                      <  1:1491814/149‑1 (MQ=255)
                gcgTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGGTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGc                                                                                                                                    <  1:1178374/149‑1 (MQ=255)
                   tACTCACCACCGCCAGCGACGACCGGAGAAACGCCGGTGTCGTGGTTTTAACCATATACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGt                                                                                                                                 <  2:1045794/149‑1 (MQ=255)
                     cTCAACACCGCCAGCGAAGACCGGAGAACCGCCGGTGTCGTGGTTTTAACCATAAACGTTGGATTTAGTGTCTGCACGACATACCATGCCACCCAGACGAGTGAAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCa                                                                                                                               <  2:193414/149‑1 (MQ=255)
                     cTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTAAGTGTCTGCCCGCCATACAATGCCACCCAGACGAGTGAAGATGTCAAGGTCGTCAGTGATTTGGTAACCAAGTTTAGCTGTCa                                                                                                                               >  1:907683/1‑149 (MQ=255)
                      tCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAg                                                                                                                              <  2:41014/149‑1 (MQ=255)
                      tCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATCTCCAGGTCCTCAGTGATTGGGTAACCCAGTTTAGCGGTCAg                                                                                                                              >  1:183075/1‑149 (MQ=255)
                       cAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGATGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGt                                                                                                                             <  1:639325/149‑1 (MQ=255)
                        aaCACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAACCGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGtt                                                                                                                            >  1:276732/1‑149 (MQ=255)
                        aaCACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACTTTGGATTTAGTGTCTGCACGCCAAACCATGCCACCCAGCCGAGAGTAGATGTCCCGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGtt                                                                                                                            >  2:1015094/1‑149 (MQ=255)
                        aaCACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGtt                                                                                                                            <  1:1077638/149‑1 (MQ=255)
                         acacCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCCCCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACGCAGTTTAGCGGTCAGTTg                                                                                                                           >  1:411587/1‑149 (MQ=255)
                           acCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGGTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGaa                                                                                                                         <  2:517561/149‑1 (MQ=255)
                             cGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGCTGTCCCGGTCGTCAGTGAGTGGGTAACCCAGTTTAGCGGTCAGTTGACCg                                                                                                                       >  1:148907/1‑149 (MQ=255)
                             cGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGACCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACg                                                                                                                       >  1:330102/1‑149 (MQ=255)
                               ccAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGcc                                                                                                                     >  1:730456/1‑149 (MQ=255)
                                   cGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGTTAACCCAGTTTAGCGGTCAGTTGAACGCCCTgc                                                                                                                 >  2:889006/1‑148 (MQ=255)
                                        aCCGGCGAACCGCCCGTGTCGTTGTTTTTACCATTAACGTTTGAATTACTGTCTGCACGCCATACCATGCCACCCATACGAGGGTATCTGTCCATGTGGGCAGTGAATTGGTAACCCAGTTTAGCGGTCAGTTGCACGCCCTGAGCttt                                                                                                            <  2:1305834/149‑1 (MQ=255)
                                        aCCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCttt                                                                                                            <  2:1454522/149‑1 (MQ=255)
                                         ccGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCCCGCCATACCATGCCACCCAGACGAGTGTAGATGTCGAGGTCGTCTGAGATTGGGTAACCCAGATTAGCGGTAAGTTCAACGCCCAGAGCTTTg                                                                                                           >  1:472952/1‑149 (MQ=255)
                                         ccGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTg                                                                                                           <  2:1138353/149‑1 (MQ=255)
                                          cGGAGAACCGCCGGTTTCGTGGTTTTTACCATATACGTTGGATTTAGTGTCTTCCCGCCATACCATGCCACCCAGACGAGTTTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGATTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑t                                                                                                         <  2:637858/149‑1 (MQ=255)
                                                               gTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTga                                                                                    <  2:638302/149‑2 (MQ=255)
                                                                 ttttACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACACAGTTTAGCGGTCAGTTGAACGCACTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCt                                                                                  >  1:1154563/1‑149 (MQ=255)
                                                                 ttttACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTACTCAGTAAAAGTTTAACAAATTTTAGCGTTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCt                                                                                  >  2:936004/1‑149 (MQ=255)
                                                                 ttgaaCCATTAACGTTGGATGTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCt                                                                                  <  2:277631/145‑1 (MQ=255)
                                                                     acctttaaCGTGGGATTTAGTGTCTGCACGCCATTCCATGCCACCCAGACGAGTGTCGATGTCCAGGTCGTCAGTGATTGGGTATCCGAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTAATCGCTGCCTTTGt                                                                              <  1:288997/143‑1 (MQ=255)
                                                                        aTAACCGTTGGATTTAGTGTCTGCACGCCATACCATGACCCCCAGACGAGTGTAGATGTCCAGGTCGACAGTGATTGGGTAACCCAGATTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCAGCCTTTGTACg                                                                           >  1:51167/1‑149 (MQ=255)
                                                                        aTAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCATGTCGTCAGTTAAATGATAAAACATTTTAGCGGGCAGTTGAACGCCCGGAGCTTTG‑TATGCACCGTTTTGGACGCTGCCTTGGTCCg                                                                           >  2:699585/1‑149 (MQ=255)
                                                                        aTAAACGTTGGATTTAGTCTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATCTCGATGTCGTCAGTAAATTTATAAACCAAATAAGCGGTCAGTTTAAAGCCCGGAGCTTTG‑TATGAACCGTGTTCAACGCTTCCTTTGTACg                                                                           >  2:988904/1‑149 (MQ=255)
                                                                         tAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCAAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTAAGTAGAACGCGCGGAGCTTTGATAT‑CACCGTTTTCAACGCTGCCTTTGAACgg                                                                          >  1:1055624/1‑149 (MQ=255)
                                                                         aaaaCGGTGGATTTAGGGGCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGGCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACgg                                                                          <  1:1499413/148‑1 (MQ=255)
                                                                              gTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGCACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATAc                                                                     >  2:1486256/1‑149 (MQ=255)
                                                                                 ggATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGAc                                                                  <  1:175454/149‑1 (MQ=255)
                                                                                  gATTTAGTGGCTGCACGCCATACCATGCCACCCAGCCGCGTGTAGATGGCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGAcc                                                                 <  2:849379/149‑1 (MQ=255)
                                                                                  gATTAAGTGTCTGCACGCCATACCATGCCCCCCAGACGAGTGTAGATGTCCAGTTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGAcc                                                                 >  1:1481422/1‑149 (MQ=255)
                                                                                   aTTTAGTGTCTGCACGCCATACCATGCCACCCAGACGACTGTAGATGACCAGGTCGTCAGTGATTGTGTAACCAAGTTTAGAGGTCAGTTGAACGCCATGAGCAATG‑AATGCACCGATTTCAACGCTGCCAATGTACGTCATACGAcca                                                                >  1:1052844/1‑148 (MQ=255)
                                                                                        gtgtCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGAATGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGCGCTTTG‑TATGCACCGTTTTCAACGCTGGCTTTGTACGGCATACGCCCTaccga                                                           >  2:1068863/1‑145 (MQ=255)
                                                                                           tCTGCACGCCATACCAGGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTc                                                        <  2:1267365/149‑1 (MQ=255)
                                                                                             tGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGt                                                      <  1:732640/149‑1 (MQ=255)
                                                                                               cACGCCATAGCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCTACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTaa                                                    <  2:1290366/149‑1 (MQ=255)
                                                                                               cACGCCATACCCTGCAACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTTGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTaa                                                    <  2:1017091/149‑1 (MQ=255)
                                                                                               cACGCCATACCATGCCACCCAGACGAGTGTAGATATCCATGGACTCAGTAATATTAAAAAAAAATTTAGCGGTCAGTTGAACTCCCTGAGATTTG‑TATTCACCGTTTTCAACGCTGCCTGTGTACGGCATACGAACTAACCAGTCGtta                                                    >  2:905201/1‑147 (MQ=255)
                                                                                                    cATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCCTCAGTGATTGTATAACACAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCAt                                               >  2:1323143/1‑149 (MQ=255)
                                                                                                       aCCATGCCACCCAGACGAGTGTAGATCTCCATGTACTCAGTAAAATTAAAAAAAAAATAAGAGGTCAGTTGAACGCCCTTCGCGTTG‑TATGCACCGTGGTCACCTCTGCCTTTGTACGGCATACGACCGAACCCGTCGTCACCCATTTc                                            >  2:1150463/1‑149 (MQ=255)
                                                                                                         cATGCCACCCAGACGAGTGTAGATCTCCATGTAGACAGTAAAATTATAAAAAATTTTAGCGGTCAGTTTAACGCCCTGAGCTTTG‑TATGCCCCGTTTTCAACGCTTCCTTTGTACGTCATAAGACCTAACCACTCGTAACACATTTCaa                                          >  2:331769/1‑149 (MQ=255)
                                                                                                          aTGCCACCCAGACGAGTGTAGATGTCCAGGGCGTCAGAGATTGTGTAACCCAGTTTAGCGTTCAGTTGAACGCCCTGAGCTTTG‑TATGCCCCGTTTTCAACGCTGCCTTTGTACGGGATACGACCTAACCAGTCGTAACCCATTTCaaa                                         <  1:1015094/149‑1 (MQ=255)
                                                                                                           tGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCCGTGAGTGGGTCACCCAGTTAAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGGCGTAACCCATTTCAAAg                                        <  1:852315/149‑1 (MQ=255)
                                                                                                              cACCCAGACGAGTGTAGATGTCCAGGTCGGCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCa                                     <  2:1392245/149‑1 (MQ=255)
                                                                                                              cACCCAGACGAGTGTAGATCTCCATGTACTCAGTAAAATTAAAAAAAAAATTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTCACCAGTCGTAACCCATTTCAAAGCCa                                     >  2:387006/1‑149 (MQ=255)
                                                                                                               aCCGAGACGAGTGTAGATGTCCAGTTCGTCTGTGATTGGTTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCaa                                    <  2:183075/149‑1 (MQ=255)
                                                                                                               aCCCAGACGAGTGTAGATCTCCTTGTACGCAGTGTATTTATAAAAAAGTTTAGCGGTCAGTTGAGGGCACTGAGCTGTG‑TATGCACCGGTTTCCACGCTGCCGATGTACGGCATACGACCTAACCAGTCGATACCCATTTCAAAGCCag                                    >  2:1475317/1‑148 (MQ=255)
                                                                                                               aCCCAGACGAGTGTAGATCTCCAGGTCGTCAGTGATTGGGAAACCAAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCACCGCTGCCTATGTACGGCATACGACCTAACGAGTCGTAACGCATTTCAACGCCaa                                    >  2:1266609/1‑149 (MQ=255)
                                                                                                                cccAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACAGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAAc                                   >  1:921910/1‑149 (MQ=255)
                                                                                                                cccAGACGAGTGTAGATCTCCATGTACTCAGTAAAATTATAAAAAAATTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAAc                                   >  2:905967/1‑149 (MQ=255)
                                                                                                                      cGAGTGTAGATGTCAAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACgg                             <  2:472952/149‑1 (MQ=255)
                                                                                                                        tgtgtAGATGTCCAGGTCGTCAGTGATTGGGTAACCGAGTTTAGCGGTCAGTTTAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGAACTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGt                           <  1:889006/148‑1 (MQ=255)
                                                                                                                            tAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAAc                       >  2:1529044/1‑149 (MQ=255)
                                                                                                                                  tCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCAATTCAAAGCCAACATACGGGTTAACCTggcg                 >  2:1145864/1‑147 (MQ=255)
                                                                                                                                      ggTAGTCAGTGATTGGGTAAACCATTTTAGCGTTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGTCAACATACGGGTTAACCTGGTAacca             <  1:54149/149‑1 (MQ=255)
                                                                                                                                           tcAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCaaa        >  1:1142946/1‑149 (MQ=255)
                                                                                                                                            cAGTGATTTGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCaaaa       <  1:671036/149‑1 (MQ=255)
                                                                                                                                            cAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCaaaa       <  2:1511462/149‑1 (MQ=255)
                                                                                                                                              gTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGc     <  1:43220/149‑1 (MQ=255)
                                                                                                                                                 aTTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAGGCAcc  >  1:685975/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTG‑TATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACC  >  NC_000913_3_hsa_pgi/1019586‑1019879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑