Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	1,164,887	G→A	G265R (GGG→AGG)	nagZ →	beta N‑acetyl‑glucosaminidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	1,164,887	0	G	A	100.0%	279.3 / NA	87	G265R (GGG→AGG)	nagZ	beta N‑acetyl‑glucosaminidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/48);  total (39/48)

ACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGA  >  NC_000913_3_hsa_pgi/1164741‑1165027                                                                                                                                                   |                                                                                                                                             aCAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCagg                                                                                                                                            >  1:833340/1‑149 (MQ=255) aCAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCagg                                                                                                                                            >  1:63045/1‑149 (MQ=255)  cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGc                                                                                                                                           <  2:1516227/149‑1 (MQ=255)  cAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGc                                                                                                                                           <  1:54632/149‑1 (MQ=255)   aGTGATGTTGATCCGCGTCCGGCGCGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAGGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGcc                                                                                                                                          >  1:930663/1‑148 (MQ=255)   aGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCAGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCa                                                                                                                                          <  1:313026/149‑1 (MQ=255)   aGTGATCTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGGTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGCCGATTTATCGATGGAAGGTGCGGCGATTATGGGCAGTTATGCCGAACGCAGGct                                                                                                                                          >  2:398576/1‑148 (MQ=255)     tGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTAATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGcaggcagg                                                                                                                                        >  1:710623/1‑149 (MQ=255)      gATGTTGATCCGCGTCCGGCGAGCGGGTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCaggcaggc                                                                                                                                       <  2:1540074/149‑1 (MQ=255)            gATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAATCCGTTTTGCGACAGGAACTGTGTTTTCACGGCGTGATTTTCTCTGACGATTTAACGATGGAAGGTGCCGCGATTATGGGCTGTTATGCCGAACGCAGGCAGGCTTCACt                                                                                                                                 >  1:1132408/1‑149 (MQ=255)            gATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACt                                                                                                                                 <  1:484475/149‑1 (MQ=255)            gATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTATGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGAAGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACt                                                                                                                                 <  1:36982/149‑1 (MQ=255)                cgcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGAt                                                                                                                             >  1:16279/1‑149 (MQ=255)                cgcgTCCGGCGAGCGGTTCTCCCAACTGGCAGAAAACCGTTTTGCGTCAGGAACTGGGTTGTGACGGCGTGATTTTCTCTGACGATTTCTCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGAt                                                                                                                             <  1:384339/149‑1 (MQ=255)                  cgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGc                                                                                                                           <  2:691833/149‑1 (MQ=255)                  cgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGc                                                                                                                           <  2:49390/149‑1 (MQ=255)                   gTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCCGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCg                                                                                                                          <  1:848160/149‑1 (MQ=255)                    tCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCTCTGGATGCgg                                                                                                                         <  1:964462/149‑1 (MQ=255)                    tCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCgg                                                                                                                         <  2:38983/149‑1 (MQ=255)                      cGGCGAGCGGTTCTCCCTACTGGGTGAAAACCGGTGTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCGCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCAATGGATGGGGGt                                                                                                                       >  2:1297206/1‑149 (MQ=255)                           cgCGGTTCTCCCTACGGGCTGTAAACCGTTTTGCGTCATGAACTGGGTTTTGACGGCGTGATATTCTCTGACGATTATTCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCATGCAGGCTTGACTGGATGCGGGTTGCGa                                                                                                                  <  2:404433/148‑1 (MQ=255)                           aGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGa                                                                                                                  >  2:828952/1‑149 (MQ=255)                               gTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATg                                                                                                              >  1:377701/1‑149 (MQ=255)                                ttCTCCCTACTGGCCGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGa                                                                                                             <  1:1514153/149‑1 (MQ=255)                                   tcCCTAATGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATcc                                                                                                          <  1:723775/149‑1 (MQ=255)                                    cccTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCt                                                                                                         >  1:753222/1‑149 (MQ=255)                                     ccTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTg                                                                                                        >  2:1453613/1‑149 (MQ=255)                                     ccTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTg                                                                                                        >  2:197298/1‑149 (MQ=255)                                      cTACTGGCTGAAGACCGTTTTGCGTCAGGCACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTgg                                                                                                       >  2:1545567/1‑149 (MQ=255)                                       tacggccTGAAATCCCTGTTGCGTCAGGAACTGGGGTTTGACGGCGCGATATTCTCTGCCGATTTCTCGCTGGAAGGTCCCGCGCGTATGGGCAGTTATGCCGAACGCAGGCAGGCGTCACTGGATGCGGGTTGCGATATGATCCTGGt                                                                                                      <  2:239415/143‑1 (MQ=255)                                          tGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTg                                                                                                   <  2:82730/149‑1 (MQ=255)                                           ggCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGc                                                                                                  <  1:1311242/149‑1 (MQ=255)                                              tGAAACCCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGGGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGTGCAGTTATGCCGAACGCAGCCAGGCTTCACTGGATGAGGGTTGCGATATGATCCTGGTCTGCaat                                                                                               >  2:438744/1‑149 (MQ=255)                                              tGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCaat                                                                                               >  2:524833/1‑149 (MQ=255)                                               gAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATATTCTCTGACGATTTATCGATGGAAGGTGCCGAGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGTTCTGCaata                                                                                              >  2:285358/1‑149 (MQ=255)                                                  aaCCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATc                                                                                           >  2:1592962/1‑149 (MQ=255)                                                  aaCCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATc                                                                                           >  1:691512/1‑149 (MQ=255)                                                    ccGTTTTGCGTCTGGAACTGGGTTTTGACGGCTTGTTTTTCTCTGACTATTTCTCGATTGAAGCTGCCGGGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGTGTTGCGATATGATCCTGGTCTGCAATAATCGt                                                                                         <  1:1508988/149‑1 (MQ=255)                                                    ccGTTTTGCGTCCTGAACTGGGTTTTGACGGCGTTATTTTCTCTGACGATTAATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTTGTCTGCAATAATCGt                                                                                         <  1:1655021/149‑1 (MQ=255)                                                     cGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTa                                                                                        >  2:1008411/1‑149 (MQ=255)                                                       ttttGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTaaa                                                                                      <  2:691512/149‑1 (MQ=255)                                                       tgttGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGGTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTaaa                                                                                      <  1:1209487/147‑1 (MQ=255)                                                                  gAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAg                                                                           >  2:46297/1‑149 (MQ=255)                                                                  gAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATAGTAAAGGGGCCGTCAg                                                                           >  2:810095/1‑149 (MQ=255)                                                                        ggTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGCAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGtt                                                                     <  1:1347440/149‑1 (MQ=255)                                                                        ggTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGtt                                                                     <  1:828952/149‑1 (MQ=255)                                                                        ggTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGtt                                                                     >  1:835944/1‑149 (MQ=255)                                                                        ggTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGtt                                                                     >  2:1171626/1‑149 (MQ=255)                                                                        ggTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGtt                                                                     <  2:29816/149‑1 (MQ=255)                                                                        ggTTTTGACGGCGTGATTTTCTCTGAAGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAAGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGGCAGCGTGtt                                                                     <  1:1142015/149‑1 (MQ=255)                                                                         gTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGCATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAATGGGCCGTCAGCGTGTTa                                                                    >  1:430102/1‑149 (MQ=255)                                                                          ttttGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAg                                                                   >  1:828461/1‑149 (MQ=255)                                                                          ttttGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAg                                                                   >  2:638563/1‑149 (MQ=255)                                                                          ttttGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATCCGGGTTGCGATATGATCATGGTCTGCAATAATCGTAAAGGGGCCGTAAGCGTGTTAg                                                                   >  2:1538873/1‑149 (MQ=255)                                                                               ccGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAAt                                                              <  1:828863/148‑1 (MQ=255)                                                                               aCGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAAt                                                              >  2:439097/1‑149 (MQ=255)                                                                               aCGGCGTGATTTTCTCTGACGATTTATCGATGGAAAGTGACGCGATTATGGGCAGATATGCCGAACGCAGGCAGGCTTCACTTTATCCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCCCCGTGTTATATAAt                                                              >  1:1226082/1‑149 (MQ=255)                                                                                cGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATc                                                             <  2:753222/149‑1 (MQ=255)                                                                                cGGCCTGCTTTTCTCTGACGATTTATCTATTGAAGGAGCCGCGAGTATGGGCAGGTATGCCGAACGCAGTCAGGGGTCACTGCATGCGGGTTGCGATATGATCCTGGTCTCCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATACTc                                                             <  2:1491217/149‑1 (MQ=255)                                                                                    gTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCCCGATTATGGGCAGTTATGCAGAACGCAGGCATGATTCACTGGATTCGGGTTGCGATATGATCCTTGTGTGAAAAAATCGTAAAGGGCACGTCACCGTGTTAGATAATCTgaa                                                         >  1:896713/1‑147 (MQ=255)                                                                                      gATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGCGCAGTTATGCCGAACGCAGGCAGGCTGCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCAc                                                       <  2:1340785/149‑1 (MQ=255)                                                                                        ttttCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAATGGGCCGTCAGCGTGTTAGATAATCTGTCACCg                                                     >  1:1068271/1‑149 (MQ=255)                                                                                             tctGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGTGCCGTCAGCGTGTTAGATAATCTGTCTCCGATctc                                                >  2:1124572/1‑147 (MQ=255)                                                                                              ctGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTTTGCCGAACGCATGCATGCTTCACTGGGTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAg                                               <  1:949249/149‑1 (MQ=255)                                                                                              ctGACGATATATCGCTGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAg                                               <  1:1477797/149‑1 (MQ=255)                                                                                               tGACGAATTATCGATGGAAGGTGCCGCGATTATGGGCAGGTATGCCGAACGCAGGCAGGCATCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAgg                                              <  2:930663/149‑1 (MQ=255)                                                                                               cgccGATTTAACGATGGAAGGTGCCGCGATTATGGGCAGTTATGCGGAACGCAGGCAGGCTTCACTGGATGGGGGTTGCGTTATGATCCTGGTCTGCAATAAACGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAgg                                              <  2:1543835/146‑1 (MQ=255)                                                                                                 aCGATTTCTCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCa                                            <  2:1167120/149‑1 (MQ=255)                                                                                                 aCGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACTGATCAAGGCa                                            >  2:672304/1‑149 (MQ=255)                                                                                                    aTTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGaa                                         >  1:1521676/1‑149 (MQ=255)                                                                                                        aTCGATGGACGGTGCCGCGAGTATGGGCCGTTTTGCCGAACGCAGGCAGGCTTCAATGGATGCGGGTGGCGATATGATCCTGGGCTGCAATAATCGTAACGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGTGCAAGGCAGAACgtg                                     <  2:1595146/149‑1 (MQ=255)                                                                                                          cGATGGAAGGTGCAGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGtt                                   <  2:16279/149‑1 (MQ=255)                                                                                                           gATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACgtgtaa                                  <  1:1592962/149‑3 (MQ=255)                                                                                                                 aGGTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTAACACGtt                            <  1:46297/149‑1 (MQ=255)                                                                                                                  ggTGCCGCGATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGttt                           <  1:1453613/149‑1 (MQ=255)                                                                                                                      ccGCGATTATGGGCAGTTATGCCGAACGCAGGCATGCTTCACTGGTTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGTCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGATACACGTTTGTAt                       <  1:285358/149‑1 (MQ=255)                                                                                                                       cgcgATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATc                      >  1:1414387/1‑149 (MQ=255)                                                                                                                        gcgATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCCCCGATCAAGGCAGAACGTGTTACACGTTTGTATCa                     <  2:377701/149‑1 (MQ=255)                                                                                                                        gcgATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCa                     >  1:1446257/1‑149 (MQ=255)                                                                                                                        gcgATTATGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCa                     >  2:1364744/1‑149 (MQ=255)                                                                                                                            ttATGGGCAGTTATGCCTAACGCAGGCAGGCGTCACTGGTTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGTGGCCGTCAGCGTGTTAGATAATCTGTCACCGCTCAAGGCAGAACGTGTTACACGTTTGTATCATaaa                 <  1:1297206/149‑1 (MQ=255)                                                                                                                               tGGGCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGt              <  1:524833/149‑1 (MQ=255)                                                                                                                                gggCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGtt             >  1:1012842/1‑149 (MQ=255)                                                                                                                                 ggCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTc            <  1:1545567/149‑1 (MQ=255)                                                                                                                                 ggCAGTTATGCCGAACGCAGGCAGGCATCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCATGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTc            >  2:696398/1‑149 (MQ=255)                                                                                                                                 ggCAGATATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTTGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTc            <  2:710623/149‑1 (MQ=255)                                                                                                                                  gCAGTTATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCa           <  1:197298/149‑1 (MQ=255)                                                                                                                                       tATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTc      <  1:1171626/149‑1 (MQ=255)                                                                                                                                       tATGCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTc      <  2:25816/149‑1 (MQ=255)                                                                                                                                          gCCGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATAAGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACTTGTTACACGTTTGTATCATAAAGCTTCATTTTcgcg   >  1:1532171/1‑149 (MQ=255)                                                                                                                                           ccGAACGCAGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTcgcgc  >  1:1289896/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                             ACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGA  >  NC_000913_3_hsa_pgi/1164741‑1165027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑