Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi2,653,3670GA20.3% 448.1 / 99.1 202intergenic (+28/‑488)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (72/89);  new base A (23/18);  total (95/107)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

TGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAC  >  NC_000913_3_hsa_pgi/2653269‑2653465
                                                                                                  |                                                                                                  
  taaCTGTACTACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCTGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAt                                                                                                 <  2:4772146/99‑1 (MQ=255)
   aaCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAtt                                                                                                >  1:3539144/1‑100 (MQ=255)
    aCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                               >  2:1683225/1‑100 (MQ=255)
    aCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAAttc                                                                                               >  1:2528/1‑99 (MQ=255)
        tACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAGTTTGGCg                                                                                           <  1:1357653/100‑1 (MQ=255)
        tACGACGGCGCATCCAGTGACTGAGAACAGCGGACCGCTTTACCGGTTGAGCCAGTGCAACAAGTATTTTGCAGGCTATAAAACACCGCCACCTTTGGCg                                                                                           <  2:3929002/100‑1 (MQ=255)
           gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTgg                                                                                             <  2:644051/95‑1 (MQ=255)
           gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                        <  1:3439375/100‑1 (MQ=255)
           gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGt                                                                                         <  1:2593652/99‑1 (MQ=255)
              ggCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttt                                                                                      <  1:2891547/99‑1 (MQ=255)
              ggCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGttttt                                                                                     <  1:1230690/100‑1 (MQ=255)
               gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGttttt                                                                                     <  2:1173108/99‑1 (MQ=255)
                cgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTa                                                                                   <  1:1203607/100‑1 (MQ=255)
                cgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTa                                                                                   <  2:3665901/100‑1 (MQ=255)
                cgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTa                                                                                   <  1:3873911/100‑1 (MQ=255)
                 gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTAt                                                                                  <  2:5519888/100‑1 (MQ=255)
                  cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAtt                                                                                 >  1:4189343/1‑100 (MQ=255)
                  cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAtt                                                                                 <  2:1938563/100‑1 (MQ=255)
                  cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTGGGCGGTTTTTTAtt                                                                                 >  1:1617050/1‑100 (MQ=255)
                      gAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGACTTTGGCGGTTTTTTATTGc                                                                               >  1:2085383/1‑98 (MQ=255)
                      gAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTGc                                                                               <  2:697704/98‑1 (MQ=255)
                               gggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGtt                                                                     <  1:1118412/99‑1 (MQ=255)
                               gggCGCGCGGGCAGATTTACCGGTGGAGCCAGTGAAATACGTATTTTACAGGCAATAAAAACCCGCCAAATTCGGCGGTTTTTTAt                                                                                  <  2:5421401/86‑1 (MQ=37)
                                    cgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAGCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCgg                                                                <  2:5972449/99‑1 (MQ=255)
                                    cgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCgg                                                                <  2:1754409/99‑1 (MQ=255)
                                        ggCTGAGTTACGGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAGCCGCCAAATTCGGTGGttttt                                                                                     <  2:5924323/74‑1 (MQ=255)
                                           aGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCca                                                        <  1:2386743/100‑1 (MQ=255)
                                            gATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcag                                                       >  2:4927020/1‑100 (MQ=255)
                                             aTTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcag                                                       <  1:1470217/99‑1 (MQ=255)
                                              tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagca                                                     <  1:1826531/100‑1 (MQ=255)
                                                tACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagcag                                                    <  2:5104067/99‑1 (MQ=255)
                                                 aCCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGt                                                  >  1:5559001/1‑100 (MQ=255)
                                                  ccGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                 >  1:3118686/1‑100 (MQ=255)
                                                  ccGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                 <  1:2463651/100‑1 (MQ=255)
                                                  ccGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGt                                                  <  2:3324963/99‑1 (MQ=255)
                                                   cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                 >  2:4509691/1‑99 (MQ=255)
                                                   cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                 >  2:4312405/1‑99 (MQ=255)
                                                    ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTCTTGCTAGTCTGGATCGCGGCCTTTCCAGAAGGTTGa                                               >  1:1723262/1‑100 (MQ=255)
                                                    ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGa                                               >  2:2780461/1‑100 (MQ=255)
                                                    ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGa                                               >  2:243047/1‑100 (MQ=255)
                                                    ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTCTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGa                                               >  1:5842716/1‑100 (MQ=255)
                                                      ttGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACt                                             <  2:5229231/100‑1 (MQ=255)
                                                         aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTCACTtgt                                          >  1:4794013/1‑100 (MQ=255)
                                                          gCCAGTGAAATAAGTCTTTTACAGGCAATAAAAAACCGCCGAATTTGGTGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgtg                                         <  1:5942317/100‑1 (MQ=255)
                                                          gCCAGTGAAATAAGTATTGTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgtg                                         >  2:1813860/1‑100 (MQ=255)
                                                           ccAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgtg                                         <  1:5619915/99‑1 (MQ=255)
                                                           ccAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgtg                                         <  2:4792056/99‑1 (MQ=255)
                                                             aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGt                                                  >  2:2729976/1‑88 (MQ=255)
                                                             aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                      >  1:3418117/1‑100 (MQ=255)
                                                             aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                      <  2:2519136/100‑1 (MQ=255)
                                                             aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                      >  1:3765243/1‑100 (MQ=255)
                                                              gTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                      >  1:4750461/1‑99 (MQ=255)
                                                               tGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                    >  2:3847816/1‑100 (MQ=255)
                                                               tGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                    <  1:1684759/100‑1 (MQ=255)
                                                               tGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                    >  1:3131841/1‑100 (MQ=255)
                                                               tGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                    <  1:2887901/100‑1 (MQ=255)
                                                                gAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACAt                                   >  1:1400170/1‑100 (MQ=255)
                                                                 aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                    <  2:835218/98‑1 (MQ=255)
                                                                 aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACAt                                   <  2:3258401/99‑1 (MQ=255)
                                                                    tAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAg                                >  2:2195228/1‑99 (MQ=255)
                                                                      aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCa                              <  1:67264/99‑1 (MQ=255)
                                                                      aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGCTACATGAGcca                             >  2:3993517/1‑98 (MQ=255)
                                                                       gTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACGTGAGCaa                             >  1:2275080/1‑99 (MQ=255)
                                                                       gTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAAc                            >  1:196194/1‑100 (MQ=255)
                                                                          ttttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAg                                                                            <  2:261710/49‑1 (MQ=255)
                                                                            ttgcAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAAc                            <  2:4479519/92‑1 (MQ=255)
                                                                            ttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                       >  2:5975879/1‑100 (MQ=255)
                                                                            ttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                       >  1:1037011/1‑100 (MQ=255)
                                                                              aCAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTg                     >  2:3942053/1‑100 (MQ=255)
                                                                               ctagCAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGc                    >  1:993262/4‑100 (MQ=37)
                                                                               cAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTg                     <  2:452523/99‑1 (MQ=255)
                                                                               cAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGc                    <  1:1315534/100‑1 (MQ=255)
                                                                                aGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCt                   <  2:4104103/100‑1 (MQ=255)
                                                                                 ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCtt                  >  1:4397589/1‑100 (MQ=255)
                                                                                 ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCtt                  >  2:4495675/1‑100 (MQ=255)
                                                                                  gCAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCtt                  >  1:3390441/1‑99 (MQ=255)
                                                                                   cAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                <  2:1398870/100‑1 (MQ=255)
                                                                                   cAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                >  1:3604090/1‑100 (MQ=255)
                                                                                    aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcac               <  2:310590/100‑1 (MQ=255)
                                                                                    aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcac               <  1:4933809/100‑1 (MQ=255)
                                                                                    aaTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGTCTTGTGTCGCATGAGCGACGCAGGGGCTTcgc               >  2:2372340/1‑98 (MQ=21)
                                                                                    aaTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                >  1:5303278/1‑99 (MQ=255)
                                                                                     aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca              <  1:4593998/100‑1 (MQ=255)
                                                                                     aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                >  2:4450580/1‑98 (MQ=255)
                                                                                     aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                >  2:3479636/1‑98 (MQ=255)
                                                                                      tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg             <  2:3414314/100‑1 (MQ=255)
                                                                                       aaaaaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg             >  1:2175161/1‑99 (MQ=255)
                                                                                        aaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGc            >  1:809119/1‑99 (MQ=255)
                                                                                        aaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGc            <  2:466952/99‑1 (MQ=255)
                                                                                        aaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGc            <  2:3494000/99‑1 (MQ=255)
                                                                                        aaaaaCCGCCAAATTCGGCGGATTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTCCTTCACAGc            >  1:3801595/1‑99 (MQ=25)
                                                                                         aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaa          <  1:4487282/100‑1 (MQ=255)
                                                                                         aaaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCa           >  1:2021127/1‑99 (MQ=255)
                                                                                           aaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaaa        <  2:622858/100‑1 (MQ=255)
                                                                                            aCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAAc       <  2:4392056/100‑1 (MQ=255)
                                                                                             ccgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAAc       >  1:5744808/1‑99 (MQ=255)
                                                                                              cgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGTCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAAc       <  2:3141888/98‑1 (MQ=255)
                                                                                                 cgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAc  <  1:2714004/100‑1 (MQ=255)
                                                                                                 cgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAc  <  1:5066314/100‑1 (MQ=255)
                                                                                                 cgAATTTGGCGGTTTTTTATGGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAc  <  2:1559819/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
TGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAC  >  NC_000913_3_hsa_pgi/2653269‑2653465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑