Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi668,7420CG99.0% 763.0 / ‑1.5 211E99Q (GAA→CAA) rsfSribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (98/109);  total (100/111)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

Only 100 of 211 total aligned reads displayed.

GGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC  >  NC_000913_3_KO_pgi/668646‑668835
                                                                                                |                                                                                             
ggTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa                                                                                            >  1:2790075/1‑100 (MQ=255)
  tACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa                                                                                            >  1:4376196/1‑98 (MQ=255)
    cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACa                                                                                          <  2:4700797/98‑1 (MQ=255)
    cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAgg                                                                                        >  1:4490654/1‑100 (MQ=255)
    cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAgg                                                                                        >  1:390882/1‑100 (MQ=255)
      cGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                       >  1:1177600/1‑99 (MQ=255)
       ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCATGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGa                                                                                     >  1:4888388/1‑100 (MQ=255)
       ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                       <  2:648456/98‑1 (MQ=255)
       ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGa                                                                                     >  1:4886576/1‑100 (MQ=255)
        gTAGAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGAc                                                                                    <  1:2506901/100‑1 (MQ=255)
         tAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACg                                                                                   <  1:1052153/100‑1 (MQ=255)
          aaaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCTGTTGATACAGGCGACgg                                                                                  >  1:4918830/1‑100 (MQ=255)
           gaaCGGGTTTGTATCCAGTCCGGCGTTTTCATTCCCACGGCGACAAGTTGCAGCTTCGCTCATTAACTCCAGAGTTTTTCCAGTTGAGACAGGCGACgg                                                                                  <  1:4181958/98‑1 (MQ=255)
           aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACgg                                                                                  <  2:2289583/99‑1 (MQ=255)
           aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACgg                                                                                  <  2:4075743/99‑1 (MQ=255)
           aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGc                                                                                 <  1:183387/100‑1 (MQ=255)
           aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGc                                                                                 <  1:4459125/100‑1 (MQ=255)
             aCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGct                                                                                <  2:4658478/99‑1 (MQ=255)
              ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctct                                                                              >  1:1917735/1‑100 (MQ=255)
              ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGct                                                                                >  2:2766506/1‑98 (MQ=255)
              ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCATCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctct                                                                              >  1:4957850/1‑100 (MQ=255)
               cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctct                                                                              >  2:24710/1‑99 (MQ=255)
                ggTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctctc                                                                             >  1:934823/1‑99 (MQ=255)
                 ggttGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctctct                                                                            <  2:3600730/97‑1 (MQ=255)
                  tttGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                          <  1:4136589/100‑1 (MQ=255)
                  tttGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                          <  1:2367607/100‑1 (MQ=255)
                     gTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCt                                                                        >  2:1665985/1‑99 (MQ=255)
                      tACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTg                                                                       >  1:3806657/1‑99 (MQ=255)
                      tACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTg                                                                       >  1:1552130/1‑99 (MQ=255)
                       aCCCAGTCCGGCCTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTg                                                                       >  2:2123264/1‑98 (MQ=255)
                       aCCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTg                                                                       >  2:972089/1‑98 (MQ=255)
                       aCCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCa                                                                     >  1:831645/1‑100 (MQ=255)
                       aCCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTGCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTg                                                                       >  2:2975831/1‑98 (MQ=255)
                        cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGc                                                                      <  2:1807362/98‑1 (MQ=255)
                        cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGc                                                                      <  2:3124859/98‑1 (MQ=255)
                        cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCa                                                                     <  2:2378063/99‑1 (MQ=255)
                        cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                    >  1:3946612/1‑100 (MQ=255)
                        cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                    <  1:465266/100‑1 (MQ=255)
                         ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                    <  2:4503528/99‑1 (MQ=255)
                          cAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATg                                                                   <  2:3370384/99‑1 (MQ=255)
                          cAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGa                                                                  <  1:178265/100‑1 (MQ=255)
                           aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGa                                                                  >  2:3129054/1‑99 (MQ=255)
                           aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGa                                                                  >  2:1372128/1‑99 (MQ=255)
                           aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGAc                                                                 <  1:4369013/100‑1 (MQ=255)
                               cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACAt                                                               <  2:146364/98‑1 (MQ=255)
                               cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATgg                                                             <  1:909013/100‑1 (MQ=255)
                               cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATg                                                              <  2:2724097/99‑1 (MQ=255)
                               cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTACCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATg                                                              >  1:4865653/1‑99 (MQ=255)
                                ggCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAGTTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATgg                                                             <  2:4829381/99‑1 (MQ=255)
                                 gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGAc                                                           <  1:1572991/100‑1 (MQ=255)
                                  cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGAc                                                           >  1:3467007/1‑99 (MQ=255)
                                  cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGAc                                                           >  1:2925949/1‑99 (MQ=255)
                                      ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAAt                                                        <  2:2127752/98‑1 (MQ=255)
                                      ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCAGTAACTCCAGAGTGTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAAt                                                        <  2:4033167/98‑1 (MQ=255)
                                       tCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATca                                                      <  1:3534781/99‑1 (MQ=255)
                                         gTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATcaca                                                    >  2:3666173/1‑99 (MQ=255)
                                         gTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATcac                                                     <  2:4837790/98‑1 (MQ=255)
                                         gTTCCCACGGCGACAAGCTGCAGCTTCACGCATTAACTCCAGAGGTTTTCCAGTTGATACAGGCGCCGGCTCTCTTCCTCCATGACATGGACAATca                                                      >  1:2029036/1‑97 (MQ=255)
                                            cccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCCCTCTTCCTGCATGACATGGACAATCACATCGc                                                >  1:3710/1‑100 (MQ=255)
                                             ccACGGCGACAAGTTGCAGCTTCATGCATTAACTCCAGAGTTTTTCCATTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                <  2:4617627/99‑1 (MQ=255)
                                             ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                               >  1:2480092/1‑100 (MQ=255)
                                             ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                <  2:3904117/99‑1 (MQ=255)
                                              cACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACTTCGcc                                               >  2:2838133/1‑99 (MQ=255)
                                                 ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa                                           <  1:3627300/100‑1 (MQ=255)
                                                  gCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa                                           <  2:4291045/99‑1 (MQ=255)
                                                    gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATc                                         <  2:4757533/99‑1 (MQ=255)
                                                     aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATcc                                        <  2:1276020/99‑1 (MQ=255)
                                                        aGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                    >  1:4000971/1‑100 (MQ=255)
                                                         gTTGCAGCTTCACGCATTCACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                   <  1:3841841/100‑1 (MQ=255)
                                                         gTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                    >  2:945528/1‑99 (MQ=255)
                                                         gTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                    >  1:1010154/1‑99 (MQ=255)
                                                         gTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                   <  1:59105/100‑1 (MQ=255)
                                                          ttGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                    >  2:1057244/1‑98 (MQ=255)
                                                            gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGTCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                   >  2:1178367/1‑97 (MQ=255)
                                                            gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                >  1:1728051/1‑100 (MQ=255)
                                                                cTTCACGCATTAACTCCAGAGTTGTTCCAGTTGATACAGGCGACGGCTCTCTTCCGCATGACATGGACAATCACATCGCCCAAATCCACGa                                    >  2:226844/1‑91 (MQ=255)
                                                                    aCGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                          <  2:4287373/98‑1 (MQ=255)
                                                                     cGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCCGTCGgc                       >  1:3966311/1‑100 (MQ=255)
                                                                       cATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCCTGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                      >  2:4372326/1‑99 (MQ=255)
                                                                         ttAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCt                    <  2:2320835/99‑1 (MQ=255)
                                                                           aaCTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGt                  >  2:3655846/1‑99 (MQ=255)
                                                                             cTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtttt               >  1:2277533/1‑100 (MQ=255)
                                                                             cTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGttt                >  2:4448114/1‑99 (MQ=255)
                                                                                cAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtttt               <  2:2260369/97‑1 (MQ=255)
                                                                                cAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCa             >  2:3534547/1‑99 (MQ=255)
                                                                                cAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAc            <  1:2253635/100‑1 (MQ=255)
                                                                                cAGAGTTTTTCCAGTGGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtttt               <  2:2906368/97‑1 (MQ=255)
                                                                                 agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAcc           >  1:1390317/1‑100 (MQ=255)
                                                                                  gagTTTTTCCCGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAATTCCACGACAATCCAGTCGGCGCTGtt                 <  2:3287363/93‑1 (MQ=255)
                                                                                  gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt          >  1:3653203/1‑100 (MQ=255)
                                                                                   agTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt         <  1:3667323/100‑1 (MQ=255)
                                                                                   agTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt          <  2:2001485/99‑1 (MQ=255)
                                                                                     tttttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc        <  2:4133217/99‑1 (MQ=255)
                                                                                       tttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAGTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCt       <  2:3364545/98‑1 (MQ=255)
                                                                                       tttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAc     >  1:1987940/1‑100 (MQ=255)
                                                                                        ttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATCGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg    >  1:4873539/1‑100 (MQ=255)
                                                                                         tCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAc     <  2:1237576/98‑1 (MQ=255)
                                                                                           cAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc  <  2:3690196/99‑1 (MQ=255)
                                                                                           cAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGc   <  2:4192779/98‑1 (MQ=255)
                                                                                             gTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc  >  2:1850769/1‑97 (MQ=255)
                                                                                                |                                                                                             
GGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC  >  NC_000913_3_KO_pgi/668646‑668835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: