Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi2,513,3510CA99.5% 670.2 / ‑3.9 187L104M (CTG→ATG) nupCnucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (90/96);  total (90/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 187 total aligned reads displayed.

AACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGG  >  NC_000913_3_KO_pgi/2513255‑2513444
                                                                                                |                                                                                             
  cGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATc                                                                                          <  1:2305929/100‑1 (MQ=255)
   gAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATc                                                                                          <  2:701101/99‑1 (MQ=255)
   gAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATc                                                                                          <  2:1416145/99‑1 (MQ=255)
     aGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCgg                                                                                        >  1:4760274/1‑99 (MQ=255)
       ggACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTa                                                                                      >  2:2377695/1‑99 (MQ=255)
       ggACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTAt                                                                                     >  1:2061273/1‑100 (MQ=255)
         aCTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtc                                                                                   <  1:4461167/100‑1 (MQ=255)
          cTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtct                                                                                  <  1:4202589/100‑1 (MQ=255)
            aaCTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                 >  1:4585202/1‑99 (MQ=255)
             aCTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                 >  1:1554531/1‑98 (MQ=255)
             aCTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTcc                                                                                <  1:825408/99‑1 (MQ=255)
             aCTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCa                                                                               <  1:3106965/100‑1 (MQ=255)
             aCTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGAGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                 >  2:4818707/1‑98 (MQ=255)
              cTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                 >  2:671420/1‑97 (MQ=255)
              cTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAg                                                                              <  1:2024743/100‑1 (MQ=255)
              cTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCAGTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAg                                                                              <  1:4139879/100‑1 (MQ=255)
                 cGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGca                                                                            <  1:4614944/99‑1 (MQ=255)
                  gTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCGCAATCGTCTTTATCTCTGCAATGATCGGTATtc                                                                                   >  2:2653220/1‑91 (MQ=255)
                  gTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGcaca                                                                          >  1:4387488/1‑100 (MQ=255)
                   tCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGcaca                                                                          <  2:2404632/99‑1 (MQ=255)
                    cTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCAGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACAt                                                                         >  2:3360845/1‑99 (MQ=255)
                     tttGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACAtt                                                                        >  2:2537677/1‑99 (MQ=255)
                     tttGGTAGCATGAATGATCAAGGCCTGGCATCCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTACCTCTGCAATGATCGGTATTCTCCAGCACAt                                                                         >  2:2612078/1‑98 (MQ=255)
                      ttGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcg                                                                      >  1:106525/1‑100 (MQ=255)
                       tGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACAt                                                                         <  2:1969752/96‑1 (MQ=255)
                        ggTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcgcg                                                                    >  1:3429886/1‑100 (MQ=255)
                        ggTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcgcg                                                                    >  1:2390844/1‑100 (MQ=255)
                         gTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcgcg                                                                    <  2:4341182/99‑1 (MQ=255)
                         gTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcgc                                                                     <  2:1878041/98‑1 (MQ=255)
                          tAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgtg                                                                  <  1:344029/100‑1 (MQ=255)
                          tAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgt                                                                   <  2:3872234/99‑1 (MQ=255)
                            gCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgtgt                                                                 <  1:824693/99‑1 (MQ=255)
                             cATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTg                                                               >  1:3564459/1‑100 (MQ=255)
                               tgaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGcc                                                             >  1:4828331/1‑100 (MQ=255)
                                naatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCg                                                            >  1:3060831/2‑100 (MQ=255)
                                gaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCg                                                            >  1:1320866/1‑100 (MQ=255)
                                  atgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGt                                                          <  1:1733952/100‑1 (MQ=255)
                                   tgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGt                                                          <  2:4389899/99‑1 (MQ=255)
                                    gaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCGATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                        >  1:4888125/1‑100 (MQ=255)
                                     aTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAGTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGtta                                                        <  2:3132563/99‑3 (MQ=255)
                                     aTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGat                                                       <  1:557140/100‑1 (MQ=255)
                                     aTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGat                                                       <  1:3438891/100‑1 (MQ=255)
                                     aTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                        >  2:4200856/1‑99 (MQ=255)
                                      tCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGat                                                       <  2:1936232/99‑1 (MQ=255)
                                             cTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgcg                                               >  1:277840/1‑100 (MQ=255)
                                             cTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgc                                                >  2:100416/1‑99 (MQ=255)
                                              tGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgcg                                               >  1:1932252/1‑99 (MQ=255)
                                                 cATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAt                                           <  1:4293308/100‑1 (MQ=255)
                                                 cATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAt                                           <  1:4645075/100‑1 (MQ=255)
                                                     cttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTgg                                        <  1:301265/99‑1 (MQ=255)
                                                       tcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGttt                                     >  1:3407780/1‑100 (MQ=255)
                                                        cttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGt                                       <  1:59621/97‑1 (MQ=255)
                                                           ccTGAACGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTAGTCTCCAGCACATTCGCGCGTTGCCGGTGATCATCCGCGCAACCGGTTTCCt                                  >  2:1712787/1‑99 (MQ=255)
                                                           ccTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTcc                                   <  1:2396980/98‑1 (MQ=255)
                                                           ccTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTc                                    <  2:2947893/97‑1 (MQ=255)
                                                           ccTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTc                                    <  1:3303499/97‑1 (MQ=255)
                                                           ccTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTCCCt                                  >  2:3433284/1‑99 (MQ=255)
                                                           ccTGAAAGTGCTGTGACCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTcc                                   <  1:4906541/98‑1 (MQ=255)
                                                              gAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGct                               >  2:515814/1‑99 (MQ=255)
                                                               aaaGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGctc                              <  2:4650924/99‑1 (MQ=255)
                                                               aaaGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGctc                              >  2:400242/1‑99 (MQ=255)
                                                                 aGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCtctc                            <  2:172090/99‑1 (MQ=255)
                                                                   tGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTcc                           >  2:250746/1‑98 (MQ=255)
                                                                     cTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaa                         <  2:3134487/98‑1 (MQ=255)
                                                                     cTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                       <  1:1187567/100‑1 (MQ=255)
                                                                     cTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                       >  1:1431432/1‑100 (MQ=255)
                                                                     cTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                       >  1:2340264/1‑100 (MQ=255)
                                                                       gtgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGt                      >  1:4370378/1‑99 (MQ=255)
                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                       <  2:3197098/97‑1 (MQ=255)
                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGt                      >  2:2791713/1‑98 (MQ=255)
                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGt                      <  1:950349/98‑1 (MQ=255)
                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGt                      >  2:4359864/1‑98 (MQ=255)
                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTc                     <  1:3204504/99‑1 (MQ=255)
                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCaa                   >  2:280189/1‑99 (MQ=255)
                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                  >  1:1065430/1‑100 (MQ=255)
                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                  <  1:4332333/100‑1 (MQ=255)
                                                                             aaTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACgg                <  2:3420992/99‑1 (MQ=255)
                                                                              aTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCa              <  1:1277168/100‑1 (MQ=255)
                                                                              aTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCa              <  1:2543652/100‑1 (MQ=255)
                                                                               tCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCa              >  2:1247337/1‑99 (MQ=255)
                                                                                cGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATg            >  1:4695620/1‑100 (MQ=255)
                                                                                 gTCTTTATCTCTGCAATGATCTGTATTCTCCAGCACATTCGCGTGTTGCCTGTGAGCACCCGCTTATTTGGTCTCCTGCTCTTTAAAGTTCACGGCaag            <  2:3250045/99‑3 (MQ=255)
                                                                                 gTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCa              <  2:2112162/97‑1 (MQ=255)
                                                                                 gTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCAt             <  2:260761/98‑1 (MQ=255)
                                                                                 gTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATgg           <  1:4222692/100‑1 (MQ=255)
                                                                                  tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATgg           <  2:274313/99‑1 (MQ=255)
                                                                                  tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCCCCAAAGTCAACGGCATgg           <  2:1684282/99‑1 (MQ=255)
                                                                                      tATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCaaa      >  1:2867472/1‑100 (MQ=255)
                                                                                      tATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCa        >  2:4685741/1‑98 (MQ=255)
                                                                                       aTCACTGCAATGATCGGTACTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCCCCACAGTCACCGGCATGGGCaca      >  2:4158318/1‑97 (MQ=255)
                                                                                        tctcTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAAc     >  2:1488522/1‑99 (MQ=255)
                                                                                          tctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGCTCATCCGCGCAATTGGTTTCCTGCTCTCCAGTGTCAACGGCATggg          >  2:2383102/1‑92 (MQ=255)
                                                                                          tctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTg   >  2:966853/1‑99 (MQ=255)
                                                                                           ctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg  >  1:4795023/1‑99 (MQ=255)
                                                                                           ctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg  >  1:3071618/1‑99 (MQ=255)
                                                                                           ctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg  >  1:3071131/1‑99 (MQ=255)
                                                                                           ctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg  >  1:1772767/1‑99 (MQ=255)
                                                                                           ctGCAATGATCGGTATTCTCCAGCACACTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg  >  1:3803467/1‑99 (MQ=255)
                                                                                            tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg  <  1:2127896/98‑1 (MQ=255)
                                                                                            tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTg   <  1:3220698/97‑1 (MQ=255)
                                                                                                |                                                                                             
AACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGG  >  NC_000913_3_KO_pgi/2513255‑2513444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: