New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3502306	78 (1.040)	9 (0.130)	9/254	8.5	11.2%	intergenic (+16/‑34)	frlB/frlC	fructoselysine‑6‑P‑deglycase/fructoselysine 3‑epimerase
	?	NC_000913_3_KO_pgi	= 3502310	70 (0.990)					intergenic (+20/‑30)	frlB/frlC	fructoselysine‑6‑P‑deglycase/fructoselysine 3‑epimerase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/3502150‑3502306 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccggcccgTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAACGGTGCCAGCCACGGGTGCAGC  <  NC_000913_3_KO_pgi/3502310‑3502164                                                                                                                                                                                                                                                                                                                  GAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCC                                                                                                                                                            <  1:59377/150‑1      TTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCC                                                                                                                                                       >  1:183667/1‑150       TCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGTTGTTCGTGCCAATGGAGTGCCTCTGCTACTACCTGTCTATTTACCAAGATCACAACCCGGATAAACGCCCCTATTACGGTGGTCTGGCGGAATATTAATCCCTCTCCCGGCCC                                                                                                                                                      >  4:182316/1‑150         GCAAGGGCTGCACCCGTGGCTGGCACCATTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTTCCGGCCCGT                                                                                                                                                    >  1:822064/1‑150                      CCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGGGCCGGGA                                                                                                                                       <  1:334648/150‑1                        GTGGCTGGCACCATTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTTCCGGCCCGTTGCCGGGCCGGGAGA                                                                                                                                     <  2:822064/150‑1                                        CTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTTCCGGCC                                                                                                                                                       <  2:343976/116‑1                                        CTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTTCCGGCC                                                                                                                                                       >  1:343976/1‑116                                                                                              CACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCA                                                                                                                   >  3:202701/1‑98                                                                                              CACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCA                                                                                                                   <  4:202701/98‑1                                                                                                CAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCC                                                                                                                                                      >  4:522494/1‑61                                                                                                CAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCC                                                                                                                                                      <  3:522494/61‑1                                                                                                                CGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGGGCCGG                                                                                                                                         <  4:239577/58‑1                                                                                                                CGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGGGCCGG                                                                                                                                         >  3:239577/1‑58                                                                                                                   TATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGG                                                                                                                                              <  2:305017/50‑1                                                                                                                   TATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGTTGCCGG                                                                                                                                              >  1:305017/1‑50                                                                                                                                                        GGCCCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAAC                       >  4:64246/1‑132                                                                                                                                                        GGCCCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAAC                       <  3:64246/132‑1                                                                                                                                                         GCCCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTACTAGCGGCGTTACTCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAACGGTGCCAGCCACGGGTGCA    >  1:646963/1‑150                                                                                                                                                           CCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAACGGTGCCAGCCACGGGTGCAGC  >  2:690321/1‑150                                                                                                                                                           CCGTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAACGGTGCCAGCCACGGGTGCAGC  >  2:563771/1‑150                                                                                                                                                                                                                                                                                                                  GAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/3502150‑3502306 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccggcccgTTGCCGGGCCGGGAGAGGGATTAATATTCCACCAGACCACCGTAATAGCGGCGTTCATCCGGGTTGTGATCTTTGTAAATAGACAGGTAGTAGCAGAGCCACTCCATTGGCACGAACATCAGGAACGGTGCCAGCCACGGGTGCAGC  <  NC_000913_3_KO_pgi/3502310‑3502164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.