New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1672798	75 (1.000)	9 (0.130)	9/250	8.4	11.2%	intergenic (+17/+22)	ydgD/mdtI	putative peptidase/multidrug efflux system transporter
	?	NC_000913_3_KO_pgi	= 1672804	73 (1.050)					intergenic (+23/+16)	ydgD/mdtI	putative peptidase/multidrug efflux system transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGC  >  NC_000913_3_KO_pgi/1672650‑1672944                                                                                                                                                     |                                                                                                                                                   aCCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGa                                                                                                                                                   >  1:359242/1‑150 (MQ=255)    gATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAg                                                                                                                                                <  4:474118/150‑1 (MQ=255)     aTGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAgc                                                                                                                                               >  4:192186/1‑150 (MQ=255)       gACGGCTCGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAgcgc                                                                                                                                             <  3:473467/150‑1 (MQ=255)        aCGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGcgcg                                                                                                                                            <  2:503369/150‑1 (MQ=255)               gCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCg                                                                                                                                     <  1:794207/150‑1 (MQ=255)                   ttaattGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCtt                                                                                                                                 >  2:277975/1‑150 (MQ=255)                   ttaattGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCtt                                                                                                                                 <  2:711543/150‑1 (MQ=255)                    taattGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtc                                                                                                                                <  1:125804/150‑1 (MQ=255)                       ttGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtcatc                                                                                                                             <  3:104266/150‑1 (MQ=255)                             tGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCaa                                                                                                                       <  2:360263/150‑1 (MQ=255)                                  aGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTc                                                                                                                  <  3:192186/150‑1 (MQ=255)                                    ttCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTGCCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCAc                                                                                                                >  3:505625/1‑150 (MQ=255)                                    tgcGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATAAACAGAAAAAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCAc                                                                                                                <  2:353038/148‑1 (MQ=255)                                     tCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCAcc                                                                                                               <  2:359242/150‑1 (MQ=255)                                        gCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATg                                                                                                            >  3:20956/1‑150 (MQ=255)                                          tCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGAt                                                                                                          <  1:88347/150‑1 (MQ=255)                                           ccTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAGGTTTCACCATGATc                                                                                                         >  4:59586/1‑150 (MQ=255)                                                   gAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTGCCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGc                                                                                                 <  4:505625/150‑1 (MQ=255)                                                   gAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGc                                                                                                 <  4:64335/150‑1 (MQ=255)                                                         tCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAg                                                                                           <  1:742014/150‑1 (MQ=255)                                                          cGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGc                                                                                          >  1:232142/1‑150 (MQ=255)                                                          cGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGc                                                                                          >  4:141314/1‑150 (MQ=255)                                                            cTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACa                                                                                            <  2:96050/146‑1 (MQ=255)                                                            cTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACa                                                                                            >  1:96050/1‑146 (MQ=255)                                                                 gcgcCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCa                                                                                   >  2:633785/1‑150 (MQ=255)                                                                    ccGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGc                                                                                <  3:425987/150‑1 (MQ=255)                                                                           ccGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCt                                                                                                                                  <  2:53152/93‑1 (MQ=255)                                                                           ccGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCt                                                                                                                                  >  1:53152/1‑93 (MQ=255)                                                                           ccGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCa                                                                         <  2:277524/150‑1 (MQ=255)                                                                             gggCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGc                                                                       >  1:729548/1‑150 (MQ=255)                                                                                ccATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtcatca                                                                                                                            >  2:262832/1‑94 (MQ=255)                                                                                ccATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtcatca                                                                                                                            <  1:262832/94‑1 (MQ=255)                                                                                ccATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCtt                                                                    >  4:173225/1‑150 (MQ=255)                                                                                  aTTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTa                                                                  >  3:509751/1‑150 (MQ=255)                                                                                  aTTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTa                                                                  <  1:64240/150‑1 (MQ=255)                                                                                     tCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGa                                                               <  2:729548/150‑1 (MQ=255)                                                                                       tGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAtt                                                             >  3:178895/1‑150 (MQ=255)                                                                                       tGTTACCGGTTTTCGCGACAAGCTGGAGCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAtt                                                             >  4:100181/1‑150 (MQ=255)                                                                                        gTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAttt                                                            <  3:100181/150‑1 (MQ=255)                                                                                         ttACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTa                                                           >  3:268707/1‑150 (MQ=255)                                                                                          tACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTaa                                                          <  1:277975/150‑1 (MQ=255)                                                                                           aCCGGTTTTCGCCACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAAc                                                         >  4:448346/1‑150 (MQ=255)                                                                                             cGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCa                                                                                                                 <  3:378346/92‑1 (MQ=255)                                                                                             cGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCa                                                                                                                 >  4:378346/1‑92 (MQ=255)                                                                                              ggTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGt                                                      <  3:448346/150‑1 (MQ=255)                                                                                               gTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAACCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCCGCCTTTACGATTTAACCGtt                                                     <  3:482866/150‑1 (MQ=255)                                                                                                    cgcgACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCa                                                <  4:268707/150‑1 (MQ=255)                                                                                                      cgACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCaaa                                              >  1:404911/1‑150 (MQ=255)                                                                                                        aCAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACa                                            <  3:141314/150‑1 (MQ=255)                                                                                                           aGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAGGTTTCACCATGATCATTCCAGCCAACCGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGa                                         <  3:59586/150‑1 (MQ=255)                                                                                                             cTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATg                                                                                                            <  4:482333/81‑1 (MQ=255)                                                                                                             cTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATg                                                                                                            >  3:482333/1‑81 (MQ=255)                                                                                                              tGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATcc                                      >  1:793709/1‑150 (MQ=255)                                                                                                                gATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCaa                                    >  1:258925/1‑150 (MQ=255)                                                                                                                      cTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCg                              <  4:20956/150‑1 (MQ=255)                                                                                                                       tGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCgg                             >  3:314629/1‑150 (MQ=255)                                                                                                                         tCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCg                                                       >  3:465337/1‑122 (MQ=255)                                                                                                                         tCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCg                                                       <  4:465337/122‑1 (MQ=255)                                                                                                                            cAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAAc                        >  1:458843/1‑150 (MQ=255)                                                                                                                                 ataatGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTggc                   <  2:38660/150‑1 (MQ=255)                                                                                                                                   aatGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAga                                                                                                 >  1:383022/1‑69 (MQ=255)                                                                                                                                   aatGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGc                                                                                                 <  2:383022/70‑1 (MQ=255)                                                                                                                                     tGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGAc                                                                                     <  1:308418/80‑1 (MQ=255)                                                                                                                                     tGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGAc                                                                                     >  2:308418/1‑80 (MQ=255)                                                                                                                                       ttCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAg                                                                                  >  4:462102/1‑81 (MQ=255)                                                                                                                                       ttCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAg                                                                                  <  3:462102/81‑1 (MQ=255)                                                                                                                                        tCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATa            <  1:633785/150‑1 (MQ=255)                                                                                                                                         cAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATAc           <  2:258925/150‑1 (MQ=255)                                                                                                                                             cGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCaaa       <  2:232142/150‑1 (MQ=255)                                                                                                                                              gCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTg                                                    >  2:255697/1‑104 (MQ=255)                                                                                                                                              gCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTg                                                    <  1:255697/104‑1 (MQ=255)                                                                                                                                                 gCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAATCTACTCAACATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCg   <  1:582300/150‑1 (MQ=255)                                                                                                                                                  cccGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGc  <  2:793709/150‑1 (MQ=255)                                                                                                                                                  cccGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGc  >  2:23094/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGC  >  NC_000913_3_KO_pgi/1672650‑1672944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.