New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3781125	60 (0.800)	10 (0.140)	10/260	8.2	15.3%	intergenic (+108/‑90)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
	?	NC_000913_3_KO_pgi	= 3781138	53 (0.730)					intergenic (+121/‑77)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCC  >  NC_000913_3_KO_pgi/3780976‑3781272                                                                                                                                                         |                                                                                                                                                     ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTc                                                                                                                                                      >  3:517168/1‑150 (MQ=255)   tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGc                                                                                                                                                    <  2:508907/150‑1 (MQ=255)         aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTGTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTcc                                                                                                                                              >  3:102668/1‑150 (MQ=255)            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCggg                                                                                                                                           <  4:406991/150‑1 (MQ=255)                 ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGagag                                                                                                                                      >  4:294942/1‑150 (MQ=255)                 ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTG‑AACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                     <  3:152960/150‑1 (MQ=255)                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                                    <  1:223531/150‑1 (MQ=255)                    tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGc                                                                                                                                   <  2:391256/150‑1 (MQ=255)                    tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGc                                                                                                                                   <  2:671683/150‑1 (MQ=255)                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                                                <  4:6213/150‑1 (MQ=255)                        gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAgggagaaa                                                                                                                               >  2:511415/1‑145 (MQ=255)                        gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                               <  1:681025/150‑1 (MQ=255)                                    ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGaaaa                                                                                                                  <  1:588181/150‑1 (MQ=255)                                      cATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAgg                                                                                                                <  1:670618/150‑1 (MQ=255)                                          gTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGc                                                                                                            >  1:260762/1‑150 (MQ=255)                                                   gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCGCAc                                                                                                   >  2:624873/1‑150 (MQ=255)                                                   gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTATT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCGCAc                                                                                                   >  3:272402/1‑150 (MQ=255)                                                   ccccccTCACCCTCCCCCCCCCCCCCGGGGGGGGGGCCCCCTC‑C‑GGCCCTGTATTTCCCCCCCCCCCCAGAACGGACACCGCCCTCTTTTTCC‑CCCCCCCCCCCCCCCGGGGGGGGGGCGGGGGCGG‑GGGAAAAAGGCCGCACTGCTCAc                                                                                                   <  4:517168/149‑1 (MQ=255)                                                        cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGa                                                                                                                     <  1:519582/127‑1 (MQ=255)                                                        cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGACCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGa                                                                                                                     >  2:519582/1‑127 (MQ=255)                                                         tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGGAAAGGCCGCACTGCTCACCAAttt                                                                                             >  2:540833/1‑150 (MQ=255)                                                          cccccTACCCCTCCCCCTCAGGGAG‑GGGGCCCTTTGG‑GGCGGTGTTGGCACCCCCTCACTCTAAAACGACACCCCCCTGTTTTTTT‑CCCCCCCCCCCCCCGGGGGGGGGGGCGGGGGGGG‑GGGAAAAAGGCCGCACTGCTCACCAAtttt                                                                                            <  4:102668/148‑1 (MQ=255)                                                                   ccTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCggg                                                                                                                                           >  1:344943/1‑95 (MQ=255)                                                                   ccTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCggg                                                                                                                                           <  2:344943/95‑1 (MQ=255)                                                                    ctctCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCCCTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACACATCTTCTGCTCTCCt                                                                                  >  2:496531/1‑150 (MQ=255)                                                                       tcCCTGAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGc                                                                                                                                                    >  1:371260/1‑82 (MQ=255)                                                                       tcCCTGAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGc                                                                                                                                                    <  2:371260/82‑1 (MQ=255)                                                                         ccTCAGGGAGAGGGGACCATT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                             >  3:75331/1‑150 (MQ=255)                                                                           tCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCTTTCTCCCCTCCGGGGAGAGGGCCGGGGTGA‑AGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                                           >  4:1105/1‑150 (MQ=255)                                                                            gaGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTc                                                                                                                                                      >  3:142857/2‑75 (MQ=255)                                                                            gaGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTc                                                                                                                                                      <  4:142857/74‑1 (MQ=255)                                                                               ggAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                                       >  1:135797/1‑150 (MQ=255)                                                                                gagaGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑TTTTTTTTTTCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                                      >  2:212060/1‑150 (MQ=255)                                                                                  gagGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCCTCTCTTTTA‑CTCCCTCGCCCAACAGGGGGGCCGGCCGGGGTTA‑GGGGAAAAAGCCGCACTGCACACCAACTTTTCGCTACCCCGCCCCCGCCCTAAg                                                                    >  2:50754/1‑150 (MQ=255)                                                                                   agGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                     >  2:763646/1‑85 (MQ=255)                                                                                   agGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                     <  1:763646/85‑1 (MQ=255)                                                                                     gggACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                 >  4:351786/1‑150 (MQ=255)                                                                                       ggccGTT‑CGGGCGGTTTATGTCCTCCCTCACTCTGAACGCACCCCGCTTTTTTTTTTT‑CTCCCCCCCCCCTCCGGGGAGGGGGCGGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                <  2:823609/148‑1 (MQ=255)                                                                                         ccGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGATGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                                              >  1:118169/1‑150 (MQ=255)                                                                                                 ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTTTCTCGCCCCTCCTGGGCGACGGCCGGGGGGA‑GGCGAAAAGGCCGCACTGCTCACCAACTTTCTCCTATCCTGCCCCCGCACTCAGGGGGCAGTACGcc                                                       >  1:552154/1‑149 (MQ=255)                                                                                                    gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                                                    >  1:605532/1‑150 (MQ=255)                                                                                                               tCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑TTTCCTTGCCGCTCCCGGGGGAGGGCCGGAGTGT‑TTAGAGAATGCCGGACTGCTCTCGAATTTTCTGCTGTCATGCCCCGGCTCTCAGCGGGCAGTATACTAACCCTCGtccctt                                         >  2:153214/1‑145 (MQ=255)                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGaaa                                                                                                                   <  2:695593/74‑1 (MQ=255)                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGaaa                                                                                                                   >  1:695593/1‑74 (MQ=255)                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                       >  4:472329/1‑150 (MQ=255)                                                                                                                       tctGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                                 >  3:358073/1‑150 (MQ=255)                                                                                                                       tctGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                                 >  2:404767/1‑150 (MQ=255)                                                                                                                          gAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaa                              >  1:32628/1‑150 (MQ=255)                                                                                                                            aaCGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAActcctgac                            >  1:769151/1‑143 (MQ=255)                                                                                                                                 cccccccctctTTTTTC‑CCCCCCCCCCCCCCGGGGGAGGGGGCGGGGGGGG‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACCAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                       <  1:443020/143‑1 (MQ=255)                                                                                                                                   cccccctctTTTTTTCCCCCCCCCCCCCGCGGGGGGGGGGGCGGGGGGGG‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc                      <  1:813119/145‑1 (MQ=255)                                                                                                                                    cGCACTCTTTTTTT‑TTTTCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                    >  2:594864/1‑150 (MQ=255)                                                                                                                                      cACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAAt                  >  3:511837/1‑150 (MQ=255)                                                                                                                                         tctTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt               >  3:456841/1‑150 (MQ=255)                                                                                                                                         tctTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt               >  4:229199/1‑150 (MQ=255)                                                                                                                                          tttttttc‑CCCCCGCGCCCCTCCGGGGGGGGGGCGGGGGGGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg              <  4:370622/149‑1 (MQ=255)                                                                                                                                          ctTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg              >  1:349638/1‑150 (MQ=255)                                                                                                                                           ttttttt‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg             >  2:532205/1‑150 (MQ=255)                                                                                                                                            tttttt‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc            >  1:641762/1‑150 (MQ=255)                                                                                                                                            tttttc‑CTCCCCCGCCCCGCGGGGGGGGGGGCGGGGGGGG‑GGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc            <  4:456841/150‑1 (MQ=255)                                                                                                                                                      ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc   >  1:591185/1‑150 (MQ=255)                                                                                                                                                       cTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                                       >  3:84024/1‑97 (MQ=255)                                                                                                                                                       cTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                                       <  4:84024/97‑1 (MQ=255)                                                                                                                                                       cTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc  >  2:212148/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                                     CCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTT‑C‑GGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTT‑CTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGA‑GGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCC  >  NC_000913_3_KO_pgi/3780976‑3781272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.