| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,840 | +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA | intergenic (‑607/‑500) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233840 | 0 (0.000) | 47 (0.960) +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA |
36/178 | 0.5 | 100% | intergenic (‑607/‑500) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233841 = | NA (NA) | intergenic (‑608/‑499) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATT > NC_000913_3_KO_pgi/4233691‑4233987 | aaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAat < 2:64405‑M2/83‑1 (MQ=255) tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc < 1:152254‑M2/87‑5 (MQ=255) tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc > 1:656635‑M2/64‑146 (MQ=255) tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag < 2:656635‑M1/147‑65 (MQ=255) ggTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggcc < 2:44734‑M1/150‑68 (MQ=255) aGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggata < 1:124036‑M1/150‑73 (MQ=255) gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag < 1:736540‑M1/150‑75 (MQ=255) gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatagtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag < 1:773881‑M1/150‑75 (MQ=255) gCTGGAGCTGCTTCCAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag < 4:180553‑M1/150‑75 (MQ=255) ggAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcg < 2:374302‑M1/150‑78 (MQ=255) gAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgt < 2:149306‑M1/150‑79 (MQ=255) ctgctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgcttaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttca < 2:292599‑M1/150‑82 (MQ=255) tgctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcac > 1:408844‑M1/1‑68 (MQ=255) gctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacg > 2:275895‑M1/1‑67 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc < 1:460336‑M1/150‑86 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc < 1:611358‑M1/150‑86 (MQ=255) ttCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccg < 3:276201‑M1/150‑93 (MQ=255) ttCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccg < 1:360404‑M1/150‑93 (MQ=255) cTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggca > 1:658457‑M1/1‑55 (MQ=255) cTATACTGTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataacgcgtggcgcggttaatcatcgtcgatatgtaggccggatgcggcgtgaacgccttatccggcc < 4:134626‑M1/150‑96 (MQ=255) aCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccg > 4:398724‑M1/1‑51 (MQ=255) tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcga < 2:387949‑M1/106‑58 (MQ=255) tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcga > 1:14497‑M1/1‑49 (MQ=255) tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcga > 1:387949‑M1/1‑49 (MQ=255) tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcga < 2:14497‑M1/106‑58 (MQ=255) tAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcct > 2:599687‑M1/1‑45 (MQ=255) gagaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga < 3:420893‑M1/150‑108 (MQ=255) gaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatg < 3:247120‑M1/150‑110 (MQ=255) gaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatg > 1:461874‑M1/1‑41 (MQ=255) aGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgac > 1:164918‑M1/1‑37 (MQ=255) ggAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacg < 2:779697‑M1/150‑115 (MQ=255) gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc > 2:563263‑M1/1‑35 (MQ=255) gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc > 1:48271‑M1/1‑35 (MQ=255) ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc > 3:325637‑M1/1‑31 (MQ=255) cGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc < 2:677147‑M1/148‑120 (MQ=255) cGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc > 1:677147‑M1/1‑29 (MQ=255) aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgt > 2:38719‑M1/1‑27 (MQ=255) aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgt < 1:38719‑M1/81‑55 (MQ=255) cTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtct < 1:245426‑M1/150‑126 (MQ=255) cAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttat < 3:398724‑M1/150‑129 (MQ=255) ggTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatca < 3:319903‑M1/150‑131 (MQ=255) gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcag > 3:130250‑M1/1‑19 (MQ=255) gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgataagacgcgaccgcgtcgcatca > 2:698813‑M1/1‑19 (MQ=255) gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgataagacgcgaccgcgtcgcatca < 1:698813‑M1/149‑131 (MQ=255) tCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcagg < 4:21992‑M1/150‑133 (MQ=255) cGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggccta > 4:112782‑M1/1‑14 (MQ=255) cGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgataagacgcgaccgcgtcgcatcaggcatc < 2:105021‑M1/150‑137 (MQ=255) ggATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctac < 1:658023‑M1/150‑138 (MQ=255) ggATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggt > 3:25124‑M1/1‑13 (MQ=255) ggATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggt < 4:25124‑M1/57‑45 (MQ=255) cccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc < 2:48271‑M1/150‑143 (MQ=255) aaTgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcgatgc < 2:380797‑M1/150‑148 (MQ=255) | CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATT > NC_000913_3_KO_pgi/4233691‑4233987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |