| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_KO_pgi | 4,183,994 | C→A | S917Y (TCT→TAT) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_KO_pgi | 4,183,994 | 0 | C | A | 98.2% | 187.9 / ‑3.4 | 55 | S917Y (TCT→TAT) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (25/29); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
GTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTCTCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAG > NC_000913_3_KO_pgi/4183850‑4184139 | gTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTgc > 1:663140/1‑150 (MQ=255) tttACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTAc < 2:429654/150‑1 (MQ=255) cATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCaaa < 2:358687/150‑1 (MQ=255) ggTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACCGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGgt < 2:83875/150‑1 (MQ=255) ggAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGACAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATc > 1:585743/1‑150 (MQ=255) ggAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATc < 3:157297/150‑1 (MQ=255) gAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATcc > 1:247973/1‑150 (MQ=255) aGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCgg < 2:485057/150‑1 (MQ=255) aCCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACg > 3:283552/1‑150 (MQ=255) ccGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACgg < 4:16546/150‑1 (MQ=255) gACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGAc > 2:485989/1‑150 (MQ=255) aTTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGtt > 2:627529/1‑150 (MQ=255) aTTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGtt > 2:116724/1‑150 (MQ=255) ggttggtAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGt < 3:401823/150‑1 (MQ=255) ttggtAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCt < 2:53466/150‑1 (MQ=255) gtaaggtaaCGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTa > 3:419513/1‑150 (MQ=255) gtaaggtaaCGCCGAAAGGTGAAACTCAGCTGACAAAAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTa < 2:386740/150‑1 (MQ=255) taaggtaaCGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAATACTCTTCTCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGTTCTTTAc > 1:322601/1‑150 (MQ=255) aggtaaCGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTc < 1:610439/150‑1 (MQ=255) aggtaaCGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTc < 3:294265/150‑1 (MQ=255) aaCGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGa < 1:604427/150‑1 (MQ=255) gCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGGACGGTTATCGACGTTCAGGTCTTTACTCGCGATgg > 1:199688/1‑150 (MQ=255) gAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGATATCGACGTTCAGGTCGTTACTCGCGATGGCGt > 2:562772/1‑150 (MQ=255) aaGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAg > 1:52413/1‑150 (MQ=255) gTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTAt > 3:171967/1‑115 (MQ=255) gTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTAt < 4:171967/115‑1 (MQ=255) tGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGaaaa < 1:323050/150‑1 (MQ=255) tGAAACTCAGCTGACCCCAGAAGAAAAACGGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGGTCAGGTCTTTACTCGCGATGGCGTAGaaaa > 4:195747/1‑150 (MQ=255) cTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACa > 1:283300/1‑150 (MQ=255) aGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACt > 2:368412/1‑125 (MQ=255) aGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACt < 1:368412/125‑1 (MQ=255) cccAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCt > 1:387541/1‑150 (MQ=255) cccAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCt > 2:473071/1‑150 (MQ=255) gaaAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATc > 2:189194/1‑150 (MQ=255) aaaaaCTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCg < 1:221896/150‑1 (MQ=255) aaaaaCTGCTGCGGGCGATCTTCGGTGAGAAAGCCTCTTACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGGATCCGGTACGGTTATCGACGTTCAGGTCGTTACGCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCg < 2:30338/150‑1 (MQ=255) aaaCTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGTTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCgaa < 2:549547/150‑1 (MQ=255) aaaCTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCgaa < 4:14758/150‑1 (MQ=255) aaaCTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCgaa < 1:627529/150‑1 (MQ=255) aaaCTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCgaa < 2:425826/150‑1 (MQ=255) cgtgcgATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCaaa < 2:444958/59‑1 (MQ=255) cgtgcgATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCaaa > 1:444958/1‑59 (MQ=255) gtgcgATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGc < 4:317858/150‑1 (MQ=255) tgcgATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCt > 1:26508/1‑150 (MQ=255) ttCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAg > 1:395733/1‑150 (MQ=255) ttCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAg > 1:395755/1‑150 (MQ=255) agaAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCt > 1:615396/1‑136 (MQ=255) agaAAGCCTCTGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCt < 2:615396/136‑1 (MQ=255) tctGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTg > 2:276222/1‑150 (MQ=255) tctGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGGCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTg > 2:479250/1‑150 (MQ=255) ctGACGTTAAAGACTCTTATCTGCGCGTGCCCGACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGACGGCGTAGAAAAAGACAAACGTGCGGTGGAAATCGAAGAAATGCAGCTCAAATAGGCGAAGAAAGACCTGt < 4:401220/150‑1 (MQ=255) ctGACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGt < 3:163640/150‑1 (MQ=255) gACGTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCt < 4:419513/150‑1 (MQ=255) gTTAAAGACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTgaa < 1:473071/150‑1 (MQ=255) gACTCTTATCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTg < 2:247973/150‑1 (MQ=255) tcttatCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAg < 1:251698/150‑1 (MQ=255) tcttatCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAg < 1:145130/150‑1 (MQ=255) | GTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAAGCCTCTGACGTTAAAGACTCTTCTCTGCGCGTACCAAACGGTGTATCCGGTACGGTTATCGACGTTCAGGTCTTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAG > NC_000913_3_KO_pgi/4183850‑4184139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |