mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SvNSRound4_19_55_S2398_L007_R1_001.good.fq | 685,176 | 96,163,102 | 100.0% | 140.3 bases | 150 bases | 96.3% |
| errors | SvNSRound4_19_55_S2398_L007_R2_001.good.fq | 685,176 | 96,163,102 | 100.0% | 140.3 bases | 150 bases | 95.0% |
| errors | SvNSRound4_19_55_S2398_L008_R1_001.good.fq | 433,830 | 61,167,768 | 100.0% | 141.0 bases | 150 bases | 96.1% |
| errors | SvNSRound4_19_55_S2398_L008_R2_001.good.fq | 433,830 | 61,167,768 | 100.0% | 141.0 bases | 150 bases | 94.3% |
| total | 2,238,012 | 314,661,740 | 100.0% | 140.6 bases | 150 bases | 95.5% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913_3_KO_pgi | 4,640,086 | 64.5 | 1.7 | 100.0% | Escherichia coli str. K-12 substr. MG1655, pgi gene KO, complete genome. |
| total | 4,640,086 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 59942 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 137 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.009 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913_3_KO_pgi | 0.80875 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 18:17:06 19 Dec 2020 | 18:19:03 19 Dec 2020 | 1 minute 57 seconds |
| Read alignment to reference genome | 18:19:04 19 Dec 2020 | 18:35:39 19 Dec 2020 | 16 minutes 35 seconds |
| Preprocessing alignments for candidate junction identification | 18:35:39 19 Dec 2020 | 18:37:11 19 Dec 2020 | 1 minute 32 seconds |
| Preliminary analysis of coverage distribution | 18:37:11 19 Dec 2020 | 18:40:34 19 Dec 2020 | 3 minutes 23 seconds |
| Identifying junction candidates | 18:40:34 19 Dec 2020 | 18:43:56 19 Dec 2020 | 3 minutes 22 seconds |
| Re-alignment to junction candidates | 18:43:56 19 Dec 2020 | 18:48:13 19 Dec 2020 | 4 minutes 17 seconds |
| Resolving best read alignments | 18:48:13 19 Dec 2020 | 18:50:42 19 Dec 2020 | 2 minutes 29 seconds |
| Creating BAM files | 18:50:42 19 Dec 2020 | 18:54:03 19 Dec 2020 | 3 minutes 21 seconds |
| Tabulating error counts | 18:54:03 19 Dec 2020 | 18:55:31 19 Dec 2020 | 1 minute 28 seconds |
| Re-calibrating base error rates | 18:55:31 19 Dec 2020 | 18:55:37 19 Dec 2020 | 6 seconds |
| Examining read alignment evidence | 18:55:37 19 Dec 2020 | 19:14:30 19 Dec 2020 | 18 minutes 53 seconds |
| Polymorphism statistics | 19:14:30 19 Dec 2020 | 19:14:31 19 Dec 2020 | 1 second |
| Output | 19:14:31 19 Dec 2020 | 19:15:00 19 Dec 2020 | 29 seconds |
| Total | 57 minutes 53 seconds | ||
