New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3386201	159 (1.050)	17 (0.120)	15/252	15.2	10.3%	intergenic (‑72/+20)	yhcO/aaeB	putative barnase inhibitor/p‑hydroxybenzoic acid efflux system component
	?	NC_000913_3_KO_pgi	= 3386206	149 (1.070)					intergenic (‑77/+15)	yhcO/aaeB	putative barnase inhibitor/p‑hydroxybenzoic acid efflux system component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTTCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/3386042‑3386201 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaaccgacgcCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTAGCCAAAC  <  NC_000913_3_KO_pgi/3386206‑3386059                                                                                                                                                                                                                                                                                                                      CTTTCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAA                                                                                                                                                                >  4:880314/1‑150  TTTCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAA                                                                                                                                                               >  4:901315/1‑150  TTTCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAA                                                                                                                                                               <  1:1117251/150‑1    TCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAA                                                                                                                                                             <  3:724374/150‑1    TCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAA                                                                                                                                                             <  1:1319204/150‑1    TCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTAAAGAAATGAAAAAA                                                                                                                                                             <  4:628978/150‑1     CGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAAC                                                                                                                                                            <  2:1533789/150‑1       CCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCGTGAAAAAACCG                                                                                                                                                          >  4:455215/1‑150       CCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAACACCG                                                                                                                                                          >  1:1426941/1‑150        CAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGA                                                                                                                                                         >  2:1531174/1‑150         AGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGAC                                                                                                                                                        >  2:1334075/1‑150          GACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTAACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACG                                                                                                                                                       >  1:369815/1‑150           ACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGC                                                                                                                                                      <  2:173170/150‑1                          AAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTT                                                                                                                                       <  2:1203656/150‑1                           AAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTT                                                                                                                                      <  1:618373/150‑1                            AGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTT                                                                                                                                     <  4:335266/150‑1                              TCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTC                                                                                                                                   <  2:784512/150‑1                              TCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTC                                                                                                                                   <  2:1410516/150‑1                                            CTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAG                                                                                                                     <  2:1495468/150‑1                                             TCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTT                                                                                                                                    <  2:1266753/134‑1                                             TCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTT                                                                                                                                    >  1:1266753/1‑134                                                    TCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTT                                                                                                                                     <  1:446424/126‑1                                                    TCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTT                                                                                                                                     >  2:446424/1‑126                                                          TCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGCCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGA                                                                                                       <  2:204674/150‑1                                                                                     TCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCG                                                                                                                   <  1:934313/111‑1                                                                                     TCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCG                                                                                                                   >  2:934313/1‑111                                                                                           GTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAA                                                                                                                                                             >  2:262767/1‑63                                                                                           GTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAA                                                                                                                                                             <  1:262767/63‑1                                                                                               CCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCAT                                                                                                                                 >  1:1557673/1‑87                                                                                               CCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCAT                                                                                                                                 <  2:1557673/87‑1                                                                                                                        CTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTAAACTAAGCGATGCACGGAAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATAATTATACCTTTGATTTTGATGAGAg                                         >  2:363118/1‑149                                                                                                                         TGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATT                                        >  1:366073/1‑150                                                                                                                                                      AAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTT           >  3:804666/1‑150                                                                                                                                                      AAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGT                >  3:239677/1‑145                                                                                                                                                      AAAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGT                <  4:239677/145‑1                                                                                                                                                       AAACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTT          >  2:518015/1‑150                                                                                                                                                        AACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTA         >  3:901315/1‑150                                                                                                                                                        AACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACA                                                                          >  2:1329957/1‑85                                                                                                                                                        AACCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACA                                                                          <  1:1329957/85‑1                                                                                                                                                          CCGACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTAGC       <  1:1046222/150‑1                                                                                                                                                             ACGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTAGCCAA    <  1:124257/150‑1                                                                                                                                                              CGCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTAGCCAAA   <  1:1003807/150‑1                                                                                                                                                               GCCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTAGCCAAAC  <  1:783807/150‑1                                                                                                                                                                                                                                                                                                                      CTTTCGCCAGACCAAAGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/3386042‑3386201 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaaccgacgcCAAAAGCGTCGGTTTTTTCATGGCTATACTTAGCGATGCACGGCAGAACTCGCCGCGAAACGTGACGGTGGCAACAGATGAATATTTATACCTTTGATTTTGATGAGATTGAGAGTCAGGAGGATTTTTATCGTGACTTTAGCCAAAC  <  NC_000913_3_KO_pgi/3386206‑3386059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.