Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)97 (0.960)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
62/182 0.6 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 156 total aligned reads displayed.

GGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGC  >  NC_000913_3_KO_pgi/4233608‑4233888
                                                                                                                                                     |                                                                                                                                   
ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa                                                                                                                                     >  1:739631/1‑150 (MQ=255)
ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa                                                                                                                                     >  1:314505/1‑150 (MQ=255)
  cGGTCAGTAGTGCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACTCTCACCATTTCGCTAACGGCACTAAAACCATCACATTTTTCTGTGGCTGGCGCTACAATCTTCCAAAGTCGCAATTCTCAAAATCAGAAGAGTATTGCTAat                                                                                                                                   <  2:1241878‑M1/150‑3 (MQ=255)
   ggTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatg                                                                                                                                  >  4:847597‑M1/1‑147 (MQ=255)
    gTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatga                                                                                                                                 >  2:272354‑M1/1‑146 (MQ=255)
       agtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaa                                                                                                                              >  2:1402256‑M1/1‑143 (MQ=255)
         tagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTGTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaac                                                                                                                            <  3:186320‑M1/150‑10 (MQ=255)
           gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                          <  3:228288‑M1/150‑12 (MQ=255)
             tCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatca                                                                                                                        <  4:841577‑M1/150‑14 (MQ=255)
                gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGGCACAATTCTCACAATCAGAAGAGTATTGCTAatgaaaaacatcaatc                                                                                                                     >  3:849103‑M1/1‑134 (MQ=255)
                   aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaa                                                                                                                  >  1:871124‑M1/1‑131 (MQ=255)
                    ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaac                                                                                                                 <  1:272354‑M1/150‑21 (MQ=255)
                      aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgc                                                                                                               >  3:558321‑M1/1‑128 (MQ=255)
                      aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgc                                                                                                               >  3:141851‑M1/1‑128 (MQ=255)
                       aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgca                                                                                                              >  2:1054536‑M1/1‑127 (MQ=255)
                         tGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcaga                                                                                                            >  2:1090683‑M1/1‑125 (MQ=255)
                            ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAACCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccg                                                                                                         <  3:991613‑M1/150‑29 (MQ=255)
                            ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccg                                                                                                         >  2:135855‑M1/1‑122 (MQ=255)
                            ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccg                                                                                                         >  1:77688‑M1/1‑122 (MQ=255)
                             tCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgc                                                                                                        >  4:868692‑M1/1‑121 (MQ=255)
                              cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgct                                                                                                       <  4:322728‑M1/150‑31 (MQ=255)
                               aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAGAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctg                                                                                                      >  1:811043‑M1/1‑119 (MQ=255)
                               aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctg                                                                                                      >  1:414477‑M1/1‑119 (MQ=255)
                               aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctg                                                                                                      >  2:470093‑M1/1‑119 (MQ=255)
                                 tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcc                                                                                                    >  1:172268‑M1/1‑117 (MQ=255)
                                    gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                                 >  1:1519725‑M1/1‑114 (MQ=255)
                                     cTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggc                                                                                                <  1:1127823‑M1/150‑38 (MQ=255)
                                        aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcagg                                                                                             <  1:505516‑M1/150‑41 (MQ=255)
                                          ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                          >  2:802881‑M1/1‑108 (MQ=255)
                                               acacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacg                                                                                      <  4:281933‑M1/150‑48 (MQ=255)
                                               acacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacg                                                                                      <  2:578851‑M1/150‑48 (MQ=255)
                                                 acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggt                                                                                    >  1:23807‑M1/1‑101 (MQ=255)
                                                    cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACACTTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgta                                                                                 <  2:1249018‑M1/150‑53 (MQ=255)
                                                     aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtag                                                                                >  1:1433768‑M1/1‑97 (MQ=255)
                                                     aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtag                                                                                >  3:812526‑M1/1‑97 (MQ=255)
                                                     aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggt                                                                                    <  3:664357‑M1/146‑50 (MQ=255)
                                                      aCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtagg                                                                               <  1:957737‑M1/150‑55 (MQ=255)
                                                       cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAat                                                                                                                                   <  3:879596‑M1/97‑3 (MQ=255)
                                                          tACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctgg                                                                           <  1:391038‑M1/150‑59 (MQ=255)
                                                             gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagc                                                                        >  4:149517‑M1/1‑89 (MQ=255)
                                                               tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                          >  2:848583‑M1/1‑87 (MQ=255)
                                                                 aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgct                                                                    <  3:954032‑M1/150‑66 (MQ=255)
                                                                 aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgct                                                                    >  4:336588‑M1/1‑85 (MQ=255)
                                                                  cGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCAAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgctt                                                                   <  2:130037‑M1/150‑67 (MQ=255)
                                                                   ggCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttc                                                                  >  2:358208‑M1/1‑83 (MQ=255)
                                                                   ggCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttc                                                                  >  1:161465‑M1/1‑83 (MQ=255)
                                                                    gCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcg                                                                 <  1:470093‑M1/150‑69 (MQ=255)
                                                                     cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcga                                                                <  4:274449‑M1/150‑70 (MQ=255)
                                                                     cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcga                                                                >  2:631473‑M1/1‑81 (MQ=255)
                                                                      aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa                                                               >  4:545357‑M1/1‑80 (MQ=255)
                                                                      aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacg                                                                                      <  2:340486‑M1/127‑48 (MQ=255)
                                                                        tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagt                                                             >  3:38607‑M1/1‑78 (MQ=255)
                                                                          aaaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                           <  2:161465‑M1/150‑75 (MQ=255)
                                                                           aaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcc                                                          <  2:914686‑M1/150‑76 (MQ=255)
                                                                            aCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcct                                                         <  1:1479743‑M1/150‑77 (MQ=255)
                                                                              cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctat                                                       >  1:2073‑M1/1‑72 (MQ=255)
                                                                                 cacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatact                                                    >  4:174939‑M1/1‑69 (MQ=255)
                                                                                  acaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactt                                                   >  1:149588‑M1/1‑68 (MQ=255)
                                                                                  acaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtag                                                                                <  2:1397867‑M1/121‑54 (MQ=255)
                                                                                    aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttc                                                 <  2:739631‑M1/150‑85 (MQ=255)
                                                                                    aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttc                                                 <  1:809884‑M1/150‑85 (MQ=255)
                                                                                     tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttct                                                <  1:1176984‑M1/150‑86 (MQ=255)
                                                                                     tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaa                                                                                                                               <  4:54119‑M1/71‑7 (MQ=255)
                                                                                       tttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctag                                              <  1:134978‑M1/150‑88 (MQ=255)
                                                                                       tttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctag                                              <  1:1402256‑M1/150‑88 (MQ=255)
                                                                                            gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaat                                         >  2:1182111‑M1/1‑58 (MQ=255)
                                                                                             tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaata                                        >  2:378230‑M1/1‑57 (MQ=255)
                                                                                               aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggc                                                                                            >  4:688881‑M1/1‑55 (MQ=255)
                                                                                               aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggc                                                                                            <  3:688881‑M1/96‑42 (MQ=255)
                                                                                                 tGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaa                                    <  1:92028‑M1/150‑98 (MQ=255)
                                                                                                   gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg                                      <  1:660072‑M1/146‑96 (MQ=255)
                                                                                                   gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg                                      >  2:660072‑M1/1‑51 (MQ=255)
                                                                                                   gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgc                                                                                                     >  1:350056‑M1/1‑51 (MQ=255)
                                                                                                   gcgcTACAATCTTCCAAAGTCACAANTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaact                                  >  3:746357‑M1/1‑51 (MQ=255)
                                                                                                      cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcg                               <  3:545357‑M1/150‑103 (MQ=255)
                                                                                                        aCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa                             <  2:871124‑M1/150‑105 (MQ=255)
                                                                                                         cAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaac                            >  2:941221‑M1/1‑45 (MQ=255)
                                                                                                         cAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggca                                                                                           >  1:1050663‑M1/1‑45 (MQ=255)
                                                                                                         cAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggca                                                                                           <  2:1050663‑M1/87‑43 (MQ=255)
                                                                                                          aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactatggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaact                           >  4:806934‑M1/1‑44 (MQ=255)
                                                                                                          aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatacttcctagagaataggaacttcgaact                           >  2:1580789‑M1/1‑44 (MQ=255)
                                                                                                            tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgc                         >  2:928341‑M1/1‑42 (MQ=255)
                                                                                                            tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactac                         <  2:77688‑M1/150‑109 (MQ=255)
                                                                                                              ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcag                       <  1:695006‑M1/150‑111 (MQ=255)
                                                                                                               tCCAAAGTCAAAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcg                               >  1:1388809‑M1/1‑39 (MQ=255)
                                                                                                               tCCAAAGTCAAAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcg                               <  2:1388809‑M1/141‑103 (MQ=255)
                                                                                                                ccAAAGTCACAATTCTCAAAATCAGAAGTGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggt                     <  1:1018374‑M1/150‑113 (MQ=255)
                                                                                                                 cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtc                    >  3:200381‑M1/1‑37 (MQ=255)
                                                                                                                      tCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggc                                                                                                >  1:853374‑M1/1‑32 (MQ=255)
                                                                                                                      tCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggc                                                                                                <  2:853374‑M1/69‑38 (MQ=255)
                                                                                                                      tCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaaaccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgctccgaagtagctatactttctagagaataggaacttcgaactgccggacgacgg               >  2:1627895‑M1/1‑32 (MQ=255)
                                                                                                                        acaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggat             >  4:316561‑M1/1‑30 (MQ=255)
                                                                                                                          aaTTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatcc           >  1:1536850‑M1/1‑28 (MQ=255)
                                                                                                                                cAAAATCAGCAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaa     >  4:125029‑M1/1‑22 (MQ=255)
                                                                                                                                   aaTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgc  <  1:723411‑M1/150‑132 (MQ=255)
                                                                                                                                   aaTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgc  <  2:523335‑M1/150‑132 (MQ=255)
                                                                                                                                   aaTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgc  >  1:523335‑M1/1‑19 (MQ=255)
                                                                                                                                      cAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                           <  1:896078‑M1/90‑75 (MQ=255)
                                                                                                                                      cAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc                                                           >  2:896078‑M1/1‑16 (MQ=255)
                                                                                                                                                ttGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcact                                                                                         >  2:724444‑M1/1‑6 (MQ=255)
                                                                                                                                                     |                                                                                                                                   
GGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGC  >  NC_000913_3_KO_pgi/4233608‑4233888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.