Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,840 +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42338400 (0.000)105 (1.040)
+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA
71/182 0.2 100% intergenic (‑607/‑500) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233841 = NA (NA)intergenic (‑608/‑499) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 118 total aligned reads displayed.

CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAA  >  NC_000913_3_KO_pgi/4233691‑4233989
                                                                                                                                                     |                                                                                                                                                     
aaaatcagaagagtattgctaatgaacaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAat                                                                                                                                                       >  3:968673‑M2/68‑150 (MQ=255)
  aatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGgaatgc                                                                                                                                                     <  1:723411‑M2/85‑3 (MQ=255)
  aatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGgaatgc                                                                                                                                                     <  2:523335‑M2/85‑3 (MQ=255)
  aatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGgaatgc                                                                                                                                                     >  1:523335‑M2/66‑148 (MQ=255)
    tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                                                                                                   <  2:1294824‑M2/87‑5 (MQ=255)
                              atcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                                                                                                   <  1:237641‑M2/87‑5 (MQ=255)
                                                            gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatat                                                                                           >  1:1682402‑M1/8‑90 (MQ=255)
                                                            gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgt                                                                                                 <  2:1553905‑M1/137‑55 (MQ=255)
                                                               ctacggtgtaGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgta                                                                                        >  3:147968‑M1/5‑87 (MQ=255)
                                                                  cggtGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggc                                                                                     >  2:1075075‑M1/2‑84 (MQ=255)
                                                                   ggTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggcc                                                                                    <  4:626972‑M1/150‑68 (MQ=255)
                                                                    gtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgct                                                                                                                                                    <  1:1183305‑M1/85‑4 (MQ=255)
                                                                       tAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggat                                                                                >  2:639447‑M1/1‑79 (MQ=255)
                                                                        aGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagngtggcgcggttantcatcgtcgatatgtaggccggata                                                                               <  1:1627895‑M1/150‑73 (MQ=255)
                                                                         ggCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataa                                                                              >  2:268292‑M1/1‑77 (MQ=255)
                                                                          gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag                                                                             >  2:19403‑M1/1‑76 (MQ=255)
                                                                          gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag                                                                             <  2:1238751‑M1/150‑75 (MQ=255)
                                                                          gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag                                                                             <  2:1536850‑M1/150‑75 (MQ=255)
                                                                              gAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtg                                                                                                                  <  2:1480156‑M1/109‑38 (MQ=255)
                                                                              gAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtg                                                                                                                  >  1:1480156‑M1/1‑72 (MQ=255)
                                                                                 ctgctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttca                                                                      <  4:394042‑M1/150‑82 (MQ=255)
                                                                                   gctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacg                                                                    >  1:302858‑M1/1‑67 (MQ=255)
                                                                                     ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcggtatgtaggccggataaggcgttcacgcc                                                                  >  2:235610‑M1/1‑65 (MQ=255)
                                                                                     ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc                                                                  <  1:1362595‑M1/150‑86 (MQ=255)
                                                                                     ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc                                                                  <  2:274484‑M1/150‑86 (MQ=255)
                                                                                      tCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccg                                                                 <  1:865473‑M1/150‑87 (MQ=255)
                                                                                          aGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatc                                                             <  2:893820‑M1/150‑91 (MQ=255)
                                                                                          aGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatc                                                             <  1:56658‑M1/150‑91 (MQ=255)
                                                                                           gTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatcc                                                            <  4:968673‑M1/150‑92 (MQ=255)
                                                                                               cTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggca                                                        >  2:1241903‑M1/1‑55 (MQ=255)
                                                                                               cTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggca                                                        >  1:273028‑M1/1‑55 (MQ=255)
                                                                                                tataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaa                                                       >  4:679980‑M1/1‑54 (MQ=255)
                                                                                                 ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                      >  2:1055858‑M1/1‑53 (MQ=255)
                                                                                                 ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                      <  2:172268‑M1/150‑98 (MQ=255)
                                                                                                 ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                      >  3:424465‑M1/1‑53 (MQ=255)
                                                                                                 ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgc                                                                <  1:1457055‑M1/140‑88 (MQ=255)
                                                                                                   aCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtc                                                                                                <  4:133348‑M1/106‑56 (MQ=255)
                                                                                                    cTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccga                                                   >  4:170841‑M1/1‑50 (MQ=255)
                                                                                                     tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgat                                                  >  1:1385704‑M1/1‑49 (MQ=255)
                                                                                                       tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                                <  4:147968‑M1/150‑104 (MQ=255)
                                                                                                       tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                                <  2:1519725‑M1/150‑104 (MQ=255)
                                                                                                         tAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcct                                              >  1:829844‑M1/1‑45 (MQ=255)
                                                                                                         tAGAGAACAGGAACTTCCAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcct                                              <  2:314505‑M1/150‑106 (MQ=255)
                                                                                                          agagAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctg                                             >  3:393990‑M1/1‑44 (MQ=255)
                                                                                                           gagaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga                                            <  3:753490‑M1/150‑108 (MQ=255)
                                                                                                            agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat                                           <  2:1257642‑M1/150‑109 (MQ=255)
                                                                                                            agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat                                           <  2:91652‑M1/150‑109 (MQ=255)
                                                                                                            agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat                                           <  2:1145609‑M1/150‑109 (MQ=255)
                                                                                                            agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat                                           <  1:1075075‑M1/150‑109 (MQ=255)
                                                                                                            agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacg                                                                    >  3:769501‑M1/1‑42 (MQ=255)
                                                                                                            agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacg                                                                    <  4:769501‑M1/125‑84 (MQ=255)
                                                                                                              aaTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggtgcggttaatcgtcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgc                                         >  1:1659429‑M1/1‑40 (MQ=255)
                                                                                                               aTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcg                                        <  1:19403‑M1/150‑112 (MQ=255)
                                                                                                               aTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcg                                        <  1:631473‑M1/150‑112 (MQ=255)
                                                                                                                tAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcga                                       >  4:87851‑M1/1‑38 (MQ=255)
                                                                                                                tAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcga                                       >  2:28945‑M1/1‑38 (MQ=255)
                                                                                                                 aGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatc                                                                                                      >  1:741061‑M1/1‑37 (MQ=255)
                                                                                                                 aGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatc                                                                                                      <  2:741061‑M1/86‑50 (MQ=255)
                                                                                                                  ggAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacg                                     <  1:358208‑M1/150‑115 (MQ=255)
                                                                                                                   gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc                                    >  1:1553240‑M1/1‑35 (MQ=255)
                                                                                                                   gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataagg                                                                            <  4:544633‑M1/110‑76 (MQ=255)
                                                                                                                     aCTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcgg                                  <  2:973623‑M1/150‑118 (MQ=255)
                                                                                                                      cTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggt                                 >  4:433060‑M1/1‑32 (MQ=255)
                                                                                                                       ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgtcgcggtc                                <  2:1682402‑M1/150‑120 (MQ=255)
                                                                                                                       ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgtt                                                                        <  2:176463‑M1/110‑80 (MQ=255)
                                                                                                                       ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgtt                                                                        >  1:176463‑M1/1‑31 (MQ=255)
                                                                                                                           aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgt                            >  2:1207183‑M1/1‑27 (MQ=255)
                                                                                                                            aCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtc                           <  3:588206‑M1/150‑125 (MQ=255)
                                                                                                                            aCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctat                                                                                                                          >  4:798038‑M1/1‑26 (MQ=255)
                                                                                                                            aCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctat                                                                                                                          <  3:798038‑M1/55‑30 (MQ=255)
                                                                                                                              tGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctt                         <  2:1302487‑M1/150‑127 (MQ=255)
                                                                                                                               gCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctta                        <  1:268292‑M1/150‑128 (MQ=255)
                                                                                                                               gCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctta                        <  3:485636‑M1/150‑128 (MQ=255)
                                                                                                                               gCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgaggcctgataagacgcgaccgcgtcgca                        >  2:1079957‑M1/1‑23 (MQ=255)
                                                                                                                                cAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttat                       <  4:1028937‑M1/150‑129 (MQ=255)
                                                                                                                                cAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttat                       <  2:273028‑M1/150‑129 (MQ=255)
                                                                                                                                 aGGGCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatc                      <  2:1516313‑M1/150‑130 (MQ=255)
                                                                                                                                  ggTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatca                     <  2:499793‑M1/150‑131 (MQ=255)
                                                                                                                                  ggTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgaggcctgataagacgcgaccgcgtcgcatca                     <  1:1079957‑M1/150‑131 (MQ=255)
                                                                                                                                   gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcag                    <  3:336588‑M1/150‑132 (MQ=255)
                                                                                                                                   gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcag                    >  2:195457‑M1/1‑19 (MQ=255)
                                                                                                                                      gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcc                 >  2:932616‑M1/1‑16 (MQ=255)
                                                                                                                                      gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcga                                                                                              <  3:1022420‑M1/73‑58 (MQ=255)
                                                                                                                                      gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcga                                                                                              >  4:1022420‑M1/1‑16 (MQ=255)
                                                                                                                                        cGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataacgcgtggcgcggttaatcatcgttgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcgta               >  1:885031‑M1/1‑14 (MQ=255)
                                                                                                                                         ggATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctac              <  1:135855‑M1/150‑138 (MQ=255)
                                                                                                                                          gATCCCTGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctaca             <  1:899611‑M1/150‑139 (MQ=255)
                                                                                                                                          gATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctaca             >  1:632549‑M1/1‑12 (MQ=255)
                                                                                                                                          gATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccgga                                                                                 >  4:650184‑M1/1‑12 (MQ=255)
                                                                                                                                          gATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccgga                                                                                 <  3:650184‑M1/82‑71 (MQ=255)
                                                                                                                                            tCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacagg           >  3:1016647‑M1/1‑10 (MQ=255)
                                                                                                                                             ccccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggt          <  1:377928‑M1/150‑142 (MQ=255)
                                                                                                                                              cccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtgggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc         >  1:1032163‑M1/1‑8 (MQ=255)
                                                                                                                                              cccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc         >  1:1054725‑M1/1‑8 (MQ=255)
                                                                                                                                              cccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc         <  1:928341‑M1/150‑143 (MQ=255)
                                                                                                                                               ccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcg        >  4:845453‑M1/1‑7 (MQ=255)
                                                                                                                                                cGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgt                                                                                                 <  1:642767‑M1/60‑55 (MQ=255)
                                                                                                                                                cGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgt                                                                                                 >  2:642767‑M1/1‑6 (MQ=255)
                                                                                                                                                     tgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcgatgccg  <  1:1182111‑M1/150‑150 (MQ=255)
                                                                                                                                                     tgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcgatgccg  <  2:632549‑M1/150‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAA  >  NC_000913_3_KO_pgi/4233691‑4233989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.