New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1672789 =145 (0.950)18 (0.130) 17/254 14.3 12.2% intergenic (+8/+31) ydgD/mdtI putative peptidase/multidrug efflux system transporter
?NC_000913_3_KO_pgi 1672815 = 126 (0.890)intergenic (+34/+5) ydgD/mdtI putative peptidase/multidrug efflux system transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

ATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACA  >  NC_000913_3_KO_pgi/1672667‑1672964
                                                                                                                                                    |                                                                                                                                                     
attaattGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCt                                                                                                                                                      <  2:1528319/150‑1 (MQ=255)
 ttaattGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCtt                                                                                                                                                     >  1:906711/1‑150 (MQ=255)
 ttaattGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCtt                                                                                                                                                     >  3:593449/1‑150 (MQ=255)
     ttGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtcatc                                                                                                                                                 <  4:315919/150‑1 (MQ=255)
     ttGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtcatc                                                                                                                                                 <  1:1366520/150‑1 (MQ=255)
      tGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTtcatca                                                                                                                                                >  2:632280/1‑150 (MQ=255)
        gggTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAgg                                                                                                                                              >  2:35048/1‑150 (MQ=255)
           tGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTGCCGGTGTTCGCGACAAGCTGGATTAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCaa                                                                                                                                           >  1:334246/1‑150 (MQ=255)
           tGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTc                                                                                                                                                       <  3:155579/138‑1 (MQ=255)
              aaaGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGtt                                                                                                                                        >  3:288661/1‑150 (MQ=255)
                  ttCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCAc                                                                                                                                    <  2:153743/150‑1 (MQ=255)
                      gCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACCAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATg                                                                                                                                >  1:263974/1‑150 (MQ=255)
                      gCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATg                                                                                                                                <  1:1479384/150‑1 (MQ=255)
                       cTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGa                                                                                                                               <  4:11890/150‑1 (MQ=255)
                            gCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCGGTTACCGGTTTTCGCGACAAGCTGGAGCAACAGTAACAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCAtt                                                                                                                          <  2:548129/150‑1 (MQ=255)
                             ccGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTc                                                                                                                                                       >  2:12814/1‑120 (MQ=255)
                             ccGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTc                                                                                                                                                       <  1:12814/120‑1 (MQ=255)
                             ccGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTc                                                                                                                         >  2:1301514/1‑150 (MQ=255)
                               gcgAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTccc                                                                                                                       >  4:852523/1‑149 (MQ=255)
                               gcgAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCa                                                                                                                       >  1:1646039/1‑150 (MQ=255)
                                      gtCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACa                                                                                                                <  1:851369/149‑1 (MQ=255)
                                      aTCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACa                                                                                                                >  1:1258309/1‑150 (MQ=255)
                                      aTCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACa                                                                                                                <  1:803091/150‑1 (MQ=255)
                                        cGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGc                                                                                                              >  1:1318258/1‑150 (MQ=255)
                                             gcgcgcCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGAc                                                                                                         <  2:1072612/150‑1 (MQ=255)
                                             gcgcgcCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGAc                                                                                                         >  2:319675/1‑150 (MQ=255)
                                                cgcCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAg                                                                                                      <  2:964885/150‑1 (MQ=255)
                                                 gcCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAgg                                                                                                     <  4:57056/150‑1 (MQ=255)
                                                  ccGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGc                                                                                                    <  3:894590/150‑1 (MQ=255)
                                                    gATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGcc                                                                                                                    >  1:573490/1‑132 (MQ=255)
                                                    gATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGcc                                                                                                                    <  2:573490/132‑1 (MQ=255)
                                                    gATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCa                                                                                                  >  1:1154733/1‑150 (MQ=255)
                                                       aaCCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATc                                                                                               <  4:585135/150‑1 (MQ=255)
                                                         ccGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTTTTCATCAGGCaa                                                                                                                                           >  3:253759/1‑104 (MQ=255)
                                                         ccGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCa                                                                                             <  1:35048/150‑1 (MQ=255)
                                                          cGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAg                                                                                            >  1:1685521/1‑150 (MQ=255)
                                                          cGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAg                                                                                            >  4:357104/1‑150 (MQ=255)
                                                             gCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCAc                                                                                                                                    <  4:732931/107‑1 (MQ=255)
                                                             gCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCAc                                                                                                                                    >  3:732931/1‑107 (MQ=255)
                                                                aTTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACGGCAAGACCAGGCCAATCCAGCCTTTa                                                                                      <  2:543913/150‑1 (MQ=255)
                                                                aTTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTa                                                                                      >  2:1318728/1‑150 (MQ=255)
                                                                aTTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTa                                                                                      >  4:155439/1‑150 (MQ=255)
                                                                 tttCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTAc                                                                                     <  3:155439/150‑1 (MQ=255)
                                                                    cTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAt                                                                                  >  1:1188747/1‑150 (MQ=255)
                                                                     cgTTACCGGTTTTCGCTACAAGCTGTATCAACCGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAtt                                                                                 <  2:1306791/149‑1 (MQ=255)
                                                                      gTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAttt                                                                                <  1:954402/150‑1 (MQ=255)
                                                                        tACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTaa                                                                              >  2:1038420/1‑150 (MQ=255)
                                                                          ccGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAgg                                                                                                     <  4:214714/125‑1 (MQ=255)
                                                                          ccGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAgg                                                                                                     >  3:214714/1‑125 (MQ=255)
                                                                          ccGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAAcc                                                                            <  2:863086/150‑1 (MQ=255)
                                                                           cGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAACATAACGATCGGACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCg                                                                           >  1:46049/1‑150 (MQ=255)
                                                                            ggTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGttt                                                                                                                                       <  3:123731/89‑1 (MQ=255)
                                                                             gTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAg                                                                                            <  4:1004021/131‑1 (MQ=255)
                                                                             gTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAg                                                                                            >  3:1004021/1‑131 (MQ=255)
                                                                             gTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGtt                                                                         <  2:466954/150‑1 (MQ=255)
                                                                             gTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGtt                                                                         <  2:1326135/150‑1 (MQ=255)
                                                                               tttCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGa                                                                       <  2:62684/150‑1 (MQ=255)
                                                                                   gcgACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCaa                                                                   >  1:219875/1‑150 (MQ=255)
                                                                                    cgACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCaaa                                                                  <  1:1301514/150‑1 (MQ=255)
                                                                                     gACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                 >  4:313799/1‑150 (MQ=255)
                                                                                        aaGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCTAACAAg                                                              >  4:812072/1‑150 (MQ=255)
                                                                                          gcgggATCAACTGTCGCAAAAATAATGTTCACACGCTGCCCGACAGCGCGGGCAGCGTCTGCATCAGGCAAGTTTCACCATGAGCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGaa                                                            >  4:123972/4‑149 (MQ=255)
                                                                                          gCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGAt                                                            <  1:817421/150‑1 (MQ=255)
                                                                                            tGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATcc                                                          <  1:1318728/150‑1 (MQ=255)
                                                                                               aTCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAc                                                       >  1:543736/1‑150 (MQ=255)
                                                                                                tCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGAc                                                                      <  4:670914/134‑1 (MQ=255)
                                                                                                  aaCTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTg                                                    >  4:239225/1‑150 (MQ=255)
                                                                                                   acagTCGCAAAAAAACTGTTCCAACGCTTCCCGCCAGCGCGGTCAGCGTCTCCATCAGGCTACTTTCACCATGATCATTCCAACCAACAGCAAGACCAGGCCACTCCAGCCTTTACGACTTAGCCGTTGACCAAACAAGATCCAACCTGc                                                   <  2:263974/147‑1 (MQ=255)
                                                                                                     tGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCgg                                                 >  1:56074/1‑150 (MQ=255)
                                                                                                       tCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGa                                                                                   >  4:255159/1‑114 (MQ=255)
                                                                                                          cAAAAGTAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAAc                                            >  4:843209/1‑150 (MQ=255)
                                                                                                          cAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAAc                                            >  4:615742/1‑150 (MQ=255)
                                                                                                           aaaaaTAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACg                                           >  2:125395/1‑150 (MQ=255)
                                                                                                            acaataatGTTCAACCGCTGCCCGACAGCGCGGGCAGCGTCTTTATCAGGCAAGTTTCACCATGATCATTCCAGCCAAGAGCAAGACGAGGCCAATCCAGCCTTTACGATTTGACCGTTGACCAACCAAGATCCACCCTGCGGCTAACGt                                          >  4:1019699/3‑150 (MQ=255)
                                                                                                                    gTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCaa                                  <  4:915409/150‑1 (MQ=255)
                                                                                                                       cAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATAc                               <  1:1038420/150‑1 (MQ=255)
                                                                                                                         aaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGa                                                                       >  3:314767/1‑108 (MQ=255)
                                                                                                                         aaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCa                             <  1:1113719/150‑1 (MQ=255)
                                                                                                                            gCTGCCCGACAGAGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAc                          >  1:473376/1‑150 (MQ=255)
                                                                                                                               gCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACCGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCg                       >  1:983717/1‑150 (MQ=255)
                                                                                                                                 ccGACAGCGCGGGCAGGGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGcc                     >  3:826865/1‑150 (MQ=255)
                                                                                                                                   gACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAt                                                                                  <  4:652070/87‑1 (MQ=255)
                                                                                                                                   gACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAt                                                                                  >  3:652070/1‑87 (MQ=255)
                                                                                                                                      aGCGCGGGCAGCGTCTTCATCAGGCCTGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCcaca                <  2:334246/150‑1 (MQ=255)
                                                                                                                                       tgtcgGGCAGCGTTTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACaa               <  1:1448694/147‑1 (MQ=255)
                                                                                                                                        cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAtt                                                                                 >  4:320093/1‑83 (MQ=255)
                                                                                                                                        cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGAtt                                                                                 <  3:320093/83‑1 (MQ=255)
                                                                                                                                        cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCGAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAAt              <  2:1154733/150‑1 (MQ=255)
                                                                                                                                        cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAAt              <  1:1241436/150‑1 (MQ=255)
                                                                                                                                        cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAAt              >  4:710778/1‑150 (MQ=255)
                                                                                                                                            ggCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCTAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCAt          <  3:812072/150‑1 (MQ=255)
                                                                                                                                              cAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATaa        <  3:950740/150‑1 (MQ=255)
                                                                                                                                              cAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATaa        <  4:802124/150‑1 (MQ=255)
                                                                                                                                              cAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACAAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATaa        >  2:460753/1‑150 (MQ=255)
                                                                                                                                                gCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGc      >  2:801460/1‑150 (MQ=255)
                                                                                                                                                  gTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCga    >  2:1354081/1‑150 (MQ=255)
                                                                                                                                                   tCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCgac   <  2:891034/150‑1 (MQ=255)
                                                                                                                                                    cTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACg                                                                                    >  1:238681/1‑68 (MQ=255)
                                                                                                                                                    cTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACg                                                                                    <  2:238681/68‑1 (MQ=255)
                                                                                                                                                    cTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAccc                        <  4:177830/128‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACA  >  NC_000913_3_KO_pgi/1672667‑1672964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.