Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 3,486,594 A→C T159P (ACT→CCT)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi3,486,5940AC99.4% 598.8 / NA 166T159P (ACT→CCT) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (82/83);  total (82/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

ACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCG  >  NC_000913_3_KO_pgi/3486448‑3486735
                                                                                                                                                  |                                                                                                                                             
aCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTc                                                                                                                                            >  3:108771/1‑150 (MQ=255)
aCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGAATGTTTCTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTc                                                                                                                                            >  1:1232323/1‑150 (MQ=255)
  ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAGTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAc                                                                                                                                          >  4:68107/1‑150 (MQ=255)
    ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAccc                                                                                                                                        <  3:968299/150‑1 (MQ=255)
        aTTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCCTTGCACAGACTATGCAGAATCCAGCAAAACAACCAGACGCTATGCCTCACCCGGAc                                                                                                                                    <  4:101981/150‑1 (MQ=255)
           cTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGt                                                                                                                                 <  2:1110215/150‑1 (MQ=255)
            tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTa                                                                                                                                >  3:12410/1‑150 (MQ=255)
            tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTa                                                                                                                                >  3:83831/1‑150 (MQ=255)
            tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTa                                                                                                                                <  3:89431/150‑1 (MQ=255)
            tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCATCGAAGTGACGGGACGAAAAGCACAAAATAAGTTGAATTTCTAAAAACAACCACACGCTATGCCTCACCCGGACGCTa                                                                                                                                >  1:580758/1‑150 (MQ=255)
            tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACGTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTa                                                                                                                                <  2:1556528/150‑1 (MQ=255)
             gATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTAt                                                                                                                               <  4:564783/150‑1 (MQ=255)
                  gTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTacctacc                                                                                                                          >  4:373580/1‑144 (MQ=255)
                        cTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGACAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCaaaa                                                                                                                    >  1:1296190/1‑150 (MQ=255)
                           cacaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTa                                                                                                                 >  4:28244/1‑150 (MQ=255)
                            acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCaaa                                                                                                                     <  2:18629/145‑1 (MQ=255)
                            acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCaaa                                                                                                                     >  1:18629/1‑145 (MQ=255)
                                     gcgTCGTCTGCTAGTCACTTCAGGTAAATTGGGCAACCTGGCGTTCTTCGTCGTGACGGGCCGCATTGCACAGACTTTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCTGGACTATATGCAAATCAAAATTACCCGTCAGGa                                                                                                       <  4:67034/150‑1 (MQ=255)
                                     gcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAgaa                                                                                                       >  4:863149/1‑148 (MQ=255)
                                       gtcgtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGaaa                                                                                                     >  1:240846/1‑150 (MQ=255)
                                          gtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCTGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                                  <  1:989943/150‑1 (MQ=255)
                                               cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                             >  4:747769/1‑150 (MQ=255)
                                               cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                             <  4:479894/150‑1 (MQ=255)
                                               cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                             >  4:748316/1‑150 (MQ=255)
                                                aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                                                                                            >  1:617377/1‑150 (MQ=255)
                                                aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                                                                                            <  4:108771/150‑1 (MQ=255)
                                                  gTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGTAAATCaaa                                                                                                                     <  4:498939/123‑1 (MQ=255)
                                                  gTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGTAAATCaaa                                                                                                                     >  3:498939/1‑123 (MQ=255)
                                                    cACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                                        <  1:1414842/150‑1 (MQ=255)
                                                     aCTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTc                                                                                       <  3:715156/150‑1 (MQ=255)
                                                     aCTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTc                                                                                       >  3:795624/1‑150 (MQ=255)
                                                       ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                     >  3:914624/1‑150 (MQ=255)
                                                       ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                     >  3:114966/1‑150 (MQ=255)
                                                       ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                     >  1:1587550/1‑150 (MQ=255)
                                                       ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                     <  2:911722/150‑1 (MQ=255)
                                                       ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                     >  2:1104062/1‑150 (MQ=255)
                                                         cAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                                   <  2:1193068/150‑1 (MQ=255)
                                                            agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                     >  3:283329/1‑145 (MQ=255)
                                                            agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGtt                                                                                >  1:515126/1‑150 (MQ=255)
                                                              aaaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtct                                                                              <  2:312434/150‑1 (MQ=255)
                                                               aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAAt                                                                                                                         <  4:298724/106‑1 (MQ=255)
                                                               aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAAt                                                                                                                         >  3:298724/1‑106 (MQ=255)
                                                               aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTTGTCAGATTGTCGGCTGTtctc                                                                             <  2:1091126/150‑1 (MQ=255)
                                                               aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                             >  2:778707/1‑150 (MQ=255)
                                                               aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                             <  1:1200035/150‑1 (MQ=255)
                                                                aGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCg                                                                            >  2:249223/1‑150 (MQ=255)
                                                                 gTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                           <  4:139191/150‑1 (MQ=255)
                                                                 gTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                           >  1:1222742/1‑150 (MQ=255)
                                                                   gggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGa                                                                         >  2:1453682/1‑150 (MQ=255)
                                                                    ggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaa                                                                        >  3:716596/1‑150 (MQ=255)
                                                                     gtaaCCTGCCGGTCCTCGCCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaaa                                                                       <  2:1232323/148‑1 (MQ=255)
                                                                     gCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaaa                                                                       >  3:638771/1‑150 (MQ=255)
                                                                         ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg                                                                                      <  2:398700/131‑1 (MQ=255)
                                                                         ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg                                                                                      >  1:398700/1‑131 (MQ=255)
                                                                         ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                                                                   <  1:1310191/150‑1 (MQ=255)
                                                                          cTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                  <  3:1018492/150‑1 (MQ=255)
                                                                          cTGGCGTTCCACGACGTGACGGGCCGCATTGCACAGACTCTGCAGAATCTGGAAAAACAAACAAACGCTATCACTCACCCAGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                  <  1:68620/150‑1 (MQ=255)
                                                                           tGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                 <  2:1299784/150‑1 (MQ=255)
                                                                                ttCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGc                                                            <  2:1282149/150‑1 (MQ=255)
                                                                                   cTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAtt                                                         <  2:262677/150‑1 (MQ=255)
                                                                                    tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCg                                                                    >  2:1255635/1‑138 (MQ=255)
                                                                                    tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCg                                                                    <  1:1255635/138‑1 (MQ=255)
                                                                                       aCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTCCCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                  >  3:1042447/1‑137 (MQ=255)
                                                                                       aCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                  <  4:1042447/137‑1 (MQ=255)
                                                                                       aCGCGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAAACAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCACTcgta                                                     <  4:579393/150‑4 (MQ=255)
                                                                                           gACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAt                                                 <  1:1330469/150‑1 (MQ=255)
                                                                                             cGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGc                                               >  2:234021/1‑150 (MQ=255)
                                                                                              gggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaaa                                                                       >  3:933919/1‑125 (MQ=255)
                                                                                              gggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaaa                                                                       <  4:933919/125‑1 (MQ=255)
                                                                                              gggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                                              >  2:118675/1‑150 (MQ=255)
                                                                                               ggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                             <  1:898572/150‑1 (MQ=255)
                                                                                                gCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTgg                                            <  2:1310215/150‑1 (MQ=255)
                                                                                                 ccGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                           >  3:207580/1‑150 (MQ=255)
                                                                                                      ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                      <  4:1567/150‑1 (MQ=255)
                                                                                                      ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                      <  2:1573257/150‑1 (MQ=255)
                                                                                                      ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATCACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                      <  1:468983/150‑1 (MQ=255)
                                                                                                         cagggACTCTTCTGAATCTGGTAAAACAACCAGACGCTATGCCTCTCCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTTTCTGCTGTGCTCGTGAAACCGTGGGACGCATGCTGAAGATGCTGGAAGATCAGa                                   <  2:580758/146‑1 (MQ=255)
                                                                                                         cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGa                                   >  4:849064/1‑150 (MQ=255)
                                                                                                                   gctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                                              <  2:1605577/129‑1 (MQ=255)
                                                                                                                    ctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc                        >  2:572773/1‑150 (MQ=255)
                                                                                                                    ctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGCCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc                        >  1:902868/1‑150 (MQ=255)
                                                                                                                        aTCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcac                    >  2:628970/1‑150 (MQ=255)
                                                                                                                         tCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca                   >  4:671447/1‑150 (MQ=255)
                                                                                                                          cTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCacac                  <  1:234021/150‑1 (MQ=255)
                                                                                                                            ggCAAAACAACCCGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACgg                <  1:1168411/150‑1 (MQ=255)
                                                                                                                             gCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                       <  2:285288/110‑1 (MQ=255)
                                                                                                                             gCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                       >  1:285288/1‑110 (MQ=255)
                                                                                                                                 aacaacCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaaaa           >  3:539559/1‑150 (MQ=255)
                                                                                                                                   caacCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAcc         <  3:748316/150‑1 (MQ=255)
                                                                                                                                    aacCAGACGCTATGCCTCACCCGGACGGTATGCAAATc                                                                                                                        >  3:489418/1‑38 (MQ=255)
                                                                                                                                    aacCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCa        >  1:1666635/1‑150 (MQ=255)
                                                                                                                                      ccAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCTGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtc      >  2:191432/1‑150 (MQ=255)
                                                                                                                                      ccAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                                  >  3:418445/1‑58 (MQ=255)
                                                                                                                                      ccAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtc      >  2:263782/1‑150 (MQ=255)
                                                                                                                                      ccAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtc      >  1:1449214/1‑150 (MQ=255)
                                                                                                                                       cAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGa                                                               <  2:1333952/92‑1 (MQ=255)
                                                                                                                                       cAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCAGATCTCCGCACACGGTAAAACCAtcg     >  4:799350/1‑150 (MQ=255)
                                                                                                                                        aGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgt    >  3:674588/1‑150 (MQ=255)
                                                                                                                                         gACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgtc   <  1:249223/150‑1 (MQ=255)
                                                                                                                                          aCGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgtcg  >  3:616839/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
ACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCG  >  NC_000913_3_KO_pgi/3486448‑3486735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: