Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,840 +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42338400 (0.000)44 (1.190)
+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA
33/164 0.2 100% intergenic (‑607/‑500) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233841 = NA (NA)intergenic (‑608/‑499) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTA  >  NC_000913_3_KO_pgi/4233705‑4233988
                                                                                                                                        |                                                                                                                                                   
gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGatat                                                                                                                                        >  2:5648‑M2/137‑150 (MQ=255)
gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGatat                                                                                                                                        >  1:345293‑M2/137‑150 (MQ=255)
  actacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                      <  2:9636‑M2/16‑1 (MQ=255)
    tacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAg                                                                                                                                    >  2:82728‑M2/133‑150 (MQ=255)
        gtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCg                                                                                                                                <  1:61426‑M2/22‑1 (MQ=255)
                   agctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                                     <  3:130589‑M2/33‑1 (MQ=255)
                           cgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                             <  2:345293‑M2/41‑1 (MQ=255)
                            gaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                            >  2:483981‑M2/109‑150 (MQ=255)
                             aagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                           >  1:63586‑M2/108‑150 (MQ=255)
                             aagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaaagctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                           >  3:327247‑M2/108‑150 (MQ=255)
                               gttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                         >  2:73831‑M2/106‑150 (MQ=255)
                                ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                        >  2:447876‑M2/105‑150 (MQ=255)
                                 tcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                       <  3:289324‑M2/47‑1 (MQ=255)
                                   ctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGa                                                                                                                              >  1:366575‑M2/102‑125 (MQ=255)
                                   ctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGa                                                                                                                              <  2:366575‑M2/24‑1 (MQ=255)
                                      tactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                                  >  3:59845‑M2/99‑150 (MQ=255)
                                        ctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                                <  2:63586‑M2/54‑1 (MQ=255)
                                               gagaataggaagttcgaactgcagggcgacggatccccggaatgctcgaccaatgttctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAACCGATGCCTGa                                                                                         >  4:359891‑M2/90‑150 (MQ=255)
                                               gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                         <  4:47004‑M2/61‑1 (MQ=255)
                                               gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                         <  4:378744‑M2/61‑1 (MQ=255)
                                                  aataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                                                                      >  2:3790‑M2/87‑150 (MQ=255)
                                                    taggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                          >  1:435509‑M2/85‑112 (MQ=255)
                                                    taggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                          <  2:435509‑M2/28‑1 (MQ=255)
                                                       gaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                                 >  2:458208‑M2/82‑150 (MQ=255)
                                                       gaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                                 >  4:79740‑M2/82‑150 (MQ=255)
                                                        aacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                                                                                <  2:662978‑M2/70‑1 (MQ=255)
                                                               aactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTgataagacgcgaccgcgt                                                                         >  2:601675‑M2/74‑143 (MQ=255)
                                                                   gcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                     <  2:440590‑M2/81‑1 (MQ=255)
                                                                      ggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCa                                                                  <  1:82728‑M2/84‑1 (MQ=255)
                                                                       gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAGGCCTGATAAGACGCGACCGCGTCGCATCAg                                                                 <  2:335737‑M2/85‑1 (MQ=255)
                                                                         ctcaccatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                               >  2:337742‑M2/64‑150 (MQ=255)
                                                                         cgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                               <  1:5648‑M2/87‑1 (MQ=255)
                                                                         cgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                               >  4:45478‑M2/64‑150 (MQ=255)
                                                                           acggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCt                                                             >  3:271794‑M2/62‑150 (MQ=255)
                                                                              gatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCAtcaggcatcgg                                                          <  1:601675‑M2/92‑6 (MQ=255)
                                                                                tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                    >  3:323034‑M2/57‑122 (MQ=255)
                                                                                tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                    <  4:322978‑M2/66‑1 (MQ=255)
                                                                                tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                    <  4:323034‑M2/66‑1 (MQ=255)
                                                                                tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                    >  3:322978‑M2/57‑122 (MQ=255)
                                                                                 ccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                       <  1:483981‑M2/95‑1 (MQ=255)
                                                                                   ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                      >  2:453321‑M2/54‑85 (MQ=255)
                                                                                   ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                      <  1:453321‑M2/32‑1 (MQ=255)
                                                                                   ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                     >  2:502712‑M2/54‑150 (MQ=255)
                                                                                    cggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                                    >  2:279687‑M2/53‑150 (MQ=255)
                                                                                        taatccgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                <  2:38088‑M2/102‑1 (MQ=255)
                                                                                          gctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                              <  1:73831‑M2/104‑1 (MQ=255)
                                                                                                 caatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                >  1:356503‑M2/40‑77 (MQ=255)
                                                                                                 caatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                <  2:356503‑M2/38‑1 (MQ=255)
                                                                                                     ggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                                   <  1:502712‑M2/115‑1 (MQ=255)
                                                                                                     ggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                                   <  3:45478‑M2/115‑1 (MQ=255)
                                                                                                       tctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa                                 >  3:173508‑M2/34‑150 (MQ=255)
                                                                                                          gattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                              <  1:57872‑M2/120‑1 (MQ=255)
                                                                                                            ttaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                            <  1:279687‑M2/122‑1 (MQ=255)
                                                                                                                 cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                       <  1:447876‑M2/127‑1 (MQ=255)
                                                                                                                  gctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTa                      >  1:149089‑M2/23‑150 (MQ=255)
                                                                                                                     ataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa                   <  1:458208‑M2/131‑1 (MQ=255)
                                                                                                                       aaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                          <  4:157568‑M2/92‑1 (MQ=255)
                                                                                                                       aaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                          >  3:157568‑M2/18‑109 (MQ=255)
                                                                                                                          gcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                   >  1:173199‑M2/15‑65 (MQ=255)
                                                                                                                          gcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                   <  2:173199‑M2/51‑1 (MQ=255)
                                                                                                                                   ggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattga     <  3:79740‑M2/145‑1 (MQ=255)
                                                                                                                                   ggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattga     <  1:337742‑M2/145‑1 (MQ=255)
                                                                                                                                    gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                        <  4:188973‑M2/126‑1 (MQ=255)
                                                                                                                                    gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                        >  3:188973‑M2/5‑130 (MQ=255)
                                                                                                                                      taATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTa  <  1:622716‑M2/148‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                   
TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTA  >  NC_000913_3_KO_pgi/4233705‑4233988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.