New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 4518917 =53 (0.920)11 (0.220) 11/222 4.4 19.8% intergenic (‑159/+188) yjhU/yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
?NC_000913_3_KO_pgi 4518956 = 43 (0.860)intergenic (‑198/+149) yjhU/yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGA  >  NC_000913_3_KO_pgi/4518810‑4519104
                                                                                                                                                   |                                                                                                                                                    
ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACt                                                                                                                                                   >  4:342779/1‑150 (MQ=255)
           aTGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                                        >  3:57815/1‑150 (MQ=255)
                gATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGCGACATGCTCCTGCTCTACAGTATTACTGAGCATACACCCATCa                                                                                                                                   >  4:15093/1‑150 (MQ=255)
                     ccAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCAt                                                                                                                              >  1:50754/1‑150 (MQ=255)
                       aGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATaa                                                                                                                            >  4:78294/1‑150 (MQ=255)
                        gTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAt                                                                                                                           <  2:448908/150‑1 (MQ=255)
                                            tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAGCAAGCATAATCGAGGGTTCAATATCGcccc                                                                                                       <  2:362579/150‑1 (MQ=255)
                                                    tCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGGGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                                                                                               >  4:246571/1‑150 (MQ=255)
                                                           cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                                        >  4:374710/1‑150 (MQ=255)
                                                               aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGGGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                    <  3:246571/150‑1 (MQ=255)
                                                                aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                                                   <  3:374710/150‑1 (MQ=255)
                                                                aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                                                   <  3:145388/150‑1 (MQ=255)
                                                                  tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt                                                                                 >  1:539065/1‑150 (MQ=255)
                                                                   aaaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc                                                                                <  3:400380/150‑1 (MQ=255)
                                                                        aGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                                                   <  2:612377/142‑1 (MQ=255)
                                                                        aGCATACCATTTGAGCGATCGCCCGCTGTCCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                                                   >  1:612377/1‑142 (MQ=255)
                                                                            tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAg                                                                       >  3:258679/1‑150 (MQ=255)
                                                                             aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGa                                                                      >  2:125902/1‑150 (MQ=255)
                                                                                  aacaGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                                                 <  2:119461/147‑1 (MQ=255)
                                                                                       cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGt                                                            <  4:57815/150‑1 (MQ=255)
                                                                                         atagctcGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                                                          <  3:302955/144‑1 (MQ=255)
                                                                                            gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcactc                                                       <  2:50754/150‑1 (MQ=255)
                                                                                             cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                                                                                                   <  2:306538/89‑1 (MQ=255)
                                                                                             cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                                                                                                   >  1:306538/1‑89 (MQ=255)
                                                                                                       ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                            <  2:182427/150‑1 (MQ=255)
                                                                                                       ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                            >  4:176540/1‑150 (MQ=255)
                                                                                                       ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATACGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                            >  1:182427/1‑150 (MQ=255)
                                                                                                         taaTACTGTAAAGCCGGAGCATGTCTCCGGCTTTACGGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggg                                          >  2:65536/3‑150 (MQ=39)
                                                                                                         ccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggg                                          >  4:97439/1‑150 (MQ=255)
                                                                                                            tACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt                                                                                 >  1:297310/1‑108 (MQ=255)
                                                                                                            tACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt                                                                                 <  2:297310/108‑1 (MQ=255)
                                                                                                            tACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa                                       >  2:162365/1‑150 (MQ=255)
                                                                                                              cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa                                     >  2:379307/1‑150 (MQ=255)
                                                                                                               tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                            >  3:13722/1‑142 (MQ=255)
                                                                                                               tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                            <  4:13722/142‑1 (MQ=255)
                                                                                                               tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAg                                    <  1:125902/150‑1 (MQ=255)
                                                                                                                    aGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaac                               >  4:415985/1‑150 (MQ=255)
                                                                                                                     gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcac                                                         <  1:385876/123‑1 (MQ=255)
                                                                                                                     gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcac                                                         >  2:385876/1‑123 (MQ=255)
                                                                                                                            gcatgtcTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAAGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGt                        >  1:529545/7‑150 (MQ=255)
                                                                                                                             catgtcTCCGGCTTTACGGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGtt                       <  1:65536/145‑1 (MQ=255)
                                                                                                                             cATGCTCCGGCTTTACAGTATTACTGCGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTg                      <  3:78294/150‑1 (MQ=255)
                                                                                                                              aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                                                                                                >  1:478276/1‑43 (MQ=255)
                                                                                                                              aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                                                                                                <  2:478276/43‑1 (MQ=255)
                                                                                                                              aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                         <  3:76040/82‑1 (MQ=255)
                                                                                                                              aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                         >  4:76040/1‑82 (MQ=255)
                                                                                                                              aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTgg                     >  4:371502/1‑150 (MQ=255)
                                                                                                                               tGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGc                    >  1:391769/1‑150 (MQ=255)
                                                                                                                                       gCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcact                                                        >  4:172751/1‑107 (MQ=255)
                                                                                                                                       gCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcact                                                        <  3:172751/107‑1 (MQ=255)
                                                                                                                                        cTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGc            <  4:262370/150‑1 (MQ=255)
                                                                                                                                            aCAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGt                                                            >  2:363077/1‑98 (MQ=255)
                                                                                                                                            aCAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGt                                                            <  1:363077/98‑1 (MQ=255)
                                                                                                                                            aCAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGa        <  3:342779/150‑1 (MQ=255)
                                                                                                                                              aGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                                                                                                  <  1:369855/58‑1 (MQ=255)
                                                                                                                                              aGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                                                                                                  >  2:369855/1‑58 (MQ=255)
                                                                                                                                                tATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCa           >  3:58516/1‑143 (MQ=255)
                                                                                                                                                tATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCa           <  4:58516/143‑1 (MQ=255)
                                                                                                                                                  ttACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGa  <  2:671018/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGA  >  NC_000913_3_KO_pgi/4518810‑4519104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.