Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 2,977,325 T→C C243R (TGT→CGT)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi2,977,3250TC95.7% 232.8 / 0.2 71C243R (TGT→CGT) galRgalactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (36/31);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCGTGCGT  >  NC_000913_3_KO_pgi/2977180‑2977465
                                                                                                                                                 |                                                                                                                                            
tCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTta                                                                                                                                          >  3:446/1‑150 (MQ=255)
      aGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGa                                                                                                                                    >  4:300634/1‑150 (MQ=255)
            ttACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAAt                                                                                                                              <  4:446/150‑1 (MQ=255)
            ttACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAAt                                                                                                                              >  1:353528/1‑150 (MQ=255)
               cGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATggc                                                                                                                           >  1:480775/1‑150 (MQ=255)
                gATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATGTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATggcg                                                                                                                          >  2:405708/1‑150 (MQ=255)
                           tGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTc                                                                                                                                 <  2:500229/132‑1 (MQ=255)
                           tGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTc                                                                                                                                 >  1:500229/1‑132 (MQ=255)
                           tGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGAt                                                                                                               >  4:228056/1‑150 (MQ=255)
                           tGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGAt                                                                                                               >  1:638989/1‑150 (MQ=255)
                               aGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGc                                                                                                           <  3:300634/150‑1 (MQ=255)
                                    tATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTtct                                                                                                      <  4:418416/150‑1 (MQ=255)
                                      ttGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGt                                                                                                                    >  2:592461/1‑134 (MQ=255)
                                      ttGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGt                                                                                                                    <  1:592461/134‑1 (MQ=255)
                                       tGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCaa                                                                                                   <  3:228056/150‑1 (MQ=255)
                                        gCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAAt                                                                                                  <  2:684979/150‑1 (MQ=255)
                                        gCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAAt                                                                                                  >  2:155807/1‑150 (MQ=255)
                                        gCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAAt                                                                                                  >  1:288075/1‑150 (MQ=255)
                                                    cGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATgg                                                                                                             >  1:344193/1‑127 (MQ=255)
                                                    cGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATgg                                                                                                             <  2:344193/127‑1 (MQ=255)
                                                          gTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGa                                                                                                                                    <  2:681263/98‑1 (MQ=255)
                                                          gTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGa                                                                                                                                    >  1:681263/1‑98 (MQ=255)
                                                           tGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTAc                                                                               >  1:365795/1‑150 (MQ=255)
                                                               aTTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTAAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCggg                                                                           >  1:135280/1‑150 (MQ=255)
                                                                tttGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGt                                                                          <  2:634581/150‑1 (MQ=255)
                                                                   ggCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGa                                                                                                                >  3:239234/1‑109 (MQ=255)
                                                                   ggCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGa                                                                                                                <  4:239234/109‑1 (MQ=255)
                                                                    gCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTgaga                                                                      >  1:159401/1‑150 (MQ=255)
                                                                     cGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGAt                                                                     >  1:111011/1‑150 (MQ=255)
                                                                      gAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGAtt                                                                    >  3:193497/1‑150 (MQ=255)
                                                                        aCCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTc                                                                  <  2:480775/150‑1 (MQ=255)
                                                                        aCCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTc                                                                  <  2:288075/150‑1 (MQ=255)
                                                                         ccAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTAAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCg                                                                 <  2:135280/150‑1 (MQ=255)
                                                                          cAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGt                                                                >  3:103937/1‑150 (MQ=255)
                                                                               gAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATggg                                                                                                            >  4:229582/1‑101 (MQ=255)
                                                                               gAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATggg                                                                                                            <  3:229582/101‑1 (MQ=255)
                                                                                      gcggcgAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATaa                                                                                             >  4:120412/1‑109 (MQ=255)
                                                                                      gcggcgAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATaa                                                                                             <  3:120412/109‑1 (MQ=255)
                                                                                         gcgAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgatg                                                 <  1:155807/150‑1 (MQ=255)
                                                                                               aGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCg                                                                 >  2:29984/1‑128 (MQ=255)
                                                                                               aGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCg                                                                 <  1:29984/128‑1 (MQ=255)
                                                                                                 gCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCtt                                                      <  3:133948/137‑1 (MQ=255)
                                                                                                 gCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCtt                                                      >  4:133948/1‑137 (MQ=255)
                                                                                                 gCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtga                                                   <  2:465329/140‑1 (MQ=255)
                                                                                                 gCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtga                                                   >  1:465329/1‑140 (MQ=255)
                                                                                                 gCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTg                                         >  2:274085/1‑150 (MQ=255)
                                                                                                   aaTGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGgt                                       <  2:111011/150‑1 (MQ=255)
                                                                                                       aCCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCa                                   <  1:274085/150‑1 (MQ=255)
                                                                                                         cGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTgatgat                                               <  3:403150/136‑1 (MQ=255)
                                                                                                         cGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAAGGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTgatgat                                               >  4:403150/1‑136 (MQ=255)
                                                                                                               tttGGGACGAGGAATAAATGTCACTGCGGTAGCCAGGTATAACGATTCAATGGCCGAAGGTGCGATGGGTGTCATCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGCGCTGGTGTCACGCTAtgt                           <  4:175794/150‑1 (MQ=255)
                                                                                                               tttGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTAtgt                           <  4:103937/150‑1 (MQ=255)
                                                                                                                ttGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTAtgtg                          >  4:58515/1‑150 (MQ=255)
                                                                                                                ttGGGACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTAtgtg                          >  1:59891/1‑150 (MQ=255)
                                                                                                                  gggACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAAtt                                                           <  4:366461/115‑1 (MQ=255)
                                                                                                                  gggACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAAtt                                                           >  3:366461/1‑115 (MQ=255)
                                                                                                                   ggACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATgg                                                                                                             >  3:198069/1‑64 (MQ=255)
                                                                                                                   ggACGAGGAAGAAATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATgg                                                                                                             <  4:198069/64‑1 (MQ=255)
                                                                                                                          gaagaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTgaga                                                                      <  3:226974/96‑1 (MQ=255)
                                                                                                                          gaagaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTgaga                                                                      >  4:226974/1‑96 (MQ=255)
                                                                                                                          gaagaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGcc                >  1:583910/1‑150 (MQ=255)
                                                                                                                            agaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGTGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTg              >  2:632580/1‑150 (MQ=255)
                                                                                                                            agaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat                                                  >  1:176790/1‑114 (MQ=255)
                                                                                                                            agaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat                                                  <  2:176790/114‑1 (MQ=255)
                                                                                                                             gaaATTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGa             <  4:193497/150‑1 (MQ=255)
                                                                                                                              aaaTTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGac            <  2:583910/150‑1 (MQ=255)
                                                                                                                              aaaTTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGac            <  2:638989/150‑1 (MQ=255)
                                                                                                                                aTTTCACTGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGacca          <  3:58515/150‑1 (MQ=255)
                                                                                                                                   tCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCg       >  2:113144/1‑150 (MQ=255)
                                                                                                                                       tGCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCGTGCg   >  2:340468/1‑150 (MQ=255)
                                                                                                                                        gCGGTAGCCCGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCGTGCGt  >  1:680528/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGACGAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTATGTGCGTCCGCGCCTGACCACCGTGCGT  >  NC_000913_3_KO_pgi/2977180‑2977465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: