Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 3,397,657 Δ9 bp coding (131‑139/1470 nt) rng ← ribonuclease G

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 33976568 (0.060)96 (0.750) 71/264 1.3 93.0% coding (140/1470 nt) rng ribonuclease G
?NC_000913_3_KO_pgi 3397666 = 7 (0.050)coding (130/1470 nt) rng ribonuclease G

Only 100 of 110 total aligned reads displayed.

GCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGC  >  NC_000913_3_KO_pgi/3397523‑3397815
                                                                                                                                               |                                                                                                                                                     
cgagatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTa                                                                                                                                                 >  1:845779‑M2/144‑150 (MQ=255)
cgagatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTa                                                                                                                                                 <  1:1424700‑M2/7‑1 (MQ=255)
  agatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGa                                                                                                                                               >  1:741905‑M2/142‑150 (MQ=255)
    atgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGata                                                                                                                                             >  1:660093‑M2/140‑150 (MQ=255)
      gtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacggcCCTTGTAGATAtt                                                                                                                                           >  3:57029‑M2/138‑150 (MQ=255)
      cGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATAtt                                                                                                                                           >  3:587139/1‑150 (MQ=255)
        cgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGc                                                                                                                                         <  3:851093‑M2/15‑1 (MQ=255)
           gcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCcta                                                                                                                                      <  1:982176‑M2/18‑1 (MQ=255)
                 tgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTc                                                                                                                                <  1:1350440‑M2/24‑1 (MQ=255)
                      tgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGt                                                                                                                           <  3:55760‑M2/29‑1 (MQ=255)
                      tgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGt                                                                                                                           <  3:876452‑M2/29‑1 (MQ=255)
                       cttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTc                                                                                                                          >  2:655109/1‑150 (MQ=255)
                        cttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTcg                                                                                                                         >  2:1328830‑M2/120‑150 (MQ=255)
                         ttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTa                                                                                                                                                 >  3:17281‑M2/119‑125 (MQ=255)
                         ttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTa                                                                                                                                                 <  4:17281‑M2/7‑1 (MQ=255)
                          tttgttcttcacccgccacacattcggtgtgcggcatgatttcggatgcatgaagaaacgcggctttatcaagctcaatatctacaaaagccgcctgcataccgggaagtgcacgacCCTTGTAGCTATTGCCTACTATTCCGCCTcgcg                                                                                                                       >  2:666733‑M2/118‑150 (MQ=255)
                           ttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCgcgc                                                                                                                      <  4:203986‑M2/34‑1 (MQ=255)
                           ttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCgcgc                                                                                                                      <  1:526663‑M2/34‑1 (MQ=255)
                           ttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCgcgc                                                                                                                      <  3:559324‑M2/34‑1 (MQ=255)
                               tcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCa                                                                                                                  >  2:1204674‑M2/113‑150 (MQ=255)
                                 ttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACg                                                                                                                >  1:956951‑M2/111‑150 (MQ=255)
                                  tcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGt                                                                                                               <  1:1333125‑M2/41‑1 (MQ=255)
                                      ccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCaa                                                                                                           >  1:618743‑M2/106‑150 (MQ=255)
                                                ttcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTc                                                                                                 <  3:850487‑M2/55‑1 (MQ=255)
                                                ttcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTc                                                                                                 >  3:311791‑M2/96‑150 (MQ=255)
                                                 tcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTcc                                                                                                <  2:1440570‑M2/56‑1 (MQ=255)
                                                   ggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTg                                                                                              <  2:371079‑M2/58‑1 (MQ=255)
                                                   ggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTg                                                                                              <  2:874214‑M2/58‑1 (MQ=255)
                                                    gtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGc                                                                                             <  4:642710‑M2/59‑1 (MQ=255)
                                                    gtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGc                                                                                             >  1:984627‑M2/92‑150 (MQ=255)
                                                     tgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCa                                                                                            <  2:660093‑M2/60‑1 (MQ=255)
                                                       tgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTTCTATTCCGCGTCGCGCCTCACGTTCAATAGGAATTTTCTGCAGa                                                                                          >  1:410426‑M2/89‑150 (MQ=255)
                                                          ggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagtcgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATa                                                                                       >  3:253342‑M2/86‑150 (MQ=255)
                                                           gcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATAc                                                                                      >  3:545080‑M2/85‑150 (MQ=255)
                                                            catgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTTACGTTCAATATGAATTTCCTGCAGAATAcc                                                                                     <  2:741905‑M2/67‑1 (MQ=255)
                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTGCAATATGAATTTCCTGCAGAATACCg                                                                                    >  1:1390134/1‑150 (MQ=255)
                                                              tgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGc                                                                                   >  4:367762‑M2/82‑150 (MQ=255)
                                                                   tcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTTCTGCAGAATACCGCCATCa                                                                              >  3:730904‑M2/77‑150 (MQ=255)
                                                                   tcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCa                                                                              <  2:8797‑M2/74‑1 (MQ=255)
                                                                   tcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCa                                                                              >  2:453853‑M2/77‑150 (MQ=255)
                                                                   tcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCa                                                                              <  1:1403982‑M2/74‑1 (MQ=255)
                                                                      gatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAata                                                                           <  3:551119‑M2/77‑1 (MQ=255)
                                                                      gatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAata                                                                           >  1:735795‑M2/74‑150 (MQ=255)
                                                                      gatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAata                                                                           >  1:44508‑M2/74‑150 (MQ=255)
                                                                           atgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGc                                                                      >  4:779491‑M2/69‑150 (MQ=255)
                                                                           atgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGc                                                                      >  4:107352‑M2/69‑150 (MQ=255)
                                                                             gaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCa                                                                    >  1:872275‑M2/67‑150 (MQ=255)
                                                                             gaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCa                                                                    >  1:243357‑M2/67‑150 (MQ=255)
                                                                               agaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACt                                                                  <  2:587322‑M2/86‑1 (MQ=255)
                                                                               agaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACt                                                                  >  4:682955‑M2/65‑150 (MQ=255)
                                                                                    cgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGt                                                             >  2:377194‑M2/60‑150 (MQ=255)
                                                                                    aTCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGt                                                             <  1:655109/150‑1 (MQ=255)
                                                                                     gcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGtt                                                            >  2:798849‑M2/59‑150 (MQ=255)
                                                                                      ccAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGttt                                                           >  4:481632/1‑150 (MQ=255)
                                                                                          tttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCAt                                                                                <  4:671757‑M2/72‑1 (MQ=255)
                                                                                          tttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCAt                                                                                >  3:671757‑M2/54‑125 (MQ=255)
                                                                                          tttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGa                                                       >  2:1193525‑M2/54‑150 (MQ=255)
                                                                                          tttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGa                                                       >  3:824956‑M2/54‑150 (MQ=255)
                                                                                           ttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGaa                                                      <  4:79462‑M2/98‑1 (MQ=255)
                                                                                            tatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAg                                                     >  2:515836‑M2/52‑150 (MQ=255)
                                                                                             atccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAgg                                                    >  1:1056708‑M2/51‑150 (MQ=255)
                                                                                              tccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGc                                                   <  2:845779‑M2/101‑1 (MQ=255)
                                                                                              tccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGc                                                   >  2:319886‑M2/50‑150 (MQ=255)
                                                                                                   cccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCgcgc                                                                                                                      <  1:743465‑M2/34‑1 (MQ=255)
                                                                                                   cccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTAc                                              <  4:545080‑M2/106‑1 (MQ=255)
                                                                                                   cccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTAc                                              >  2:361891‑M2/45‑150 (MQ=255)
                                                                                                     caatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGTAGGCGTTACGt                                            >  1:525507‑M2/43‑150 (MQ=255)
                                                                                                     caatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGt                                            >  2:33505‑M2/43‑150 (MQ=255)
                                                                                                       atatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGttt                                          <  1:1328830‑M2/110‑1 (MQ=255)
                                                                                                       atatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGttt                                          >  4:601870‑M2/41‑150 (MQ=255)
                                                                                                        tatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCCCTCGCGTTTCCGAAGGCGTTACGTTTa                                         >  1:63309‑M2/40‑150 (MQ=255)
                                                                                                            tacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                                                                                                          <  1:380820‑M2/46‑1 (MQ=255)
                                                                                                            tacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                                                                                                          >  2:380820‑M2/36‑81 (MQ=255)
                                                                                                             acaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAAc                                    >  1:958271‑M2/35‑150 (MQ=255)
                                                                                                              caaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACa                                   >  4:503644‑M2/34‑150 (MQ=255)
                                                                                                               aaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACaa                                  >  1:373718‑M2/33‑150 (MQ=255)
                                                                                                                 gCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAAtt                                >  1:458603/1‑150 (MQ=255)
                                                                                                                 aagccgcctgcatacccggaagtacacgacCCTTGTAGat                                                                                                                                              <  1:456937‑M2/10‑1 (MQ=255)
                                                                                                                 aagccgcctgcatacccggaagtacacgacCCTTGTAGat                                                                                                                                              >  2:456937‑M2/31‑40 (MQ=255)
                                                                                                                 aagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAAtt                                >  2:361374‑M2/31‑150 (MQ=255)
                                                                                                                   gccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCa                              <  2:543755‑M2/122‑1 (MQ=255)
                                                                                                                      gcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGcc                           >  3:620482‑M2/26‑150 (MQ=255)
                                                                                                                      gcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGcc                           >  2:1347021‑M2/26‑150 (MQ=255)
                                                                                                                        ctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGt                         >  2:1108452‑M2/24‑150 (MQ=255)
                                                                                                                         tgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTc                        <  2:735795‑M2/128‑1 (MQ=255)
                                                                                                                           catacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt                      <  3:367762‑M2/130‑1 (MQ=255)
                                                                                                                           catacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt                      <  1:1204674‑M2/130‑1 (MQ=255)
                                                                                                                           catacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt                      <  2:547233‑M2/130‑1 (MQ=255)
                                                                                                                           aaGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt                      <  1:134761/150‑1 (MQ=255)
                                                                                                                              acccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGtt                   <  3:682955‑M2/133‑1 (MQ=255)
                                                                                                                               cccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGttt                  <  3:253090‑M2/134‑1 (MQ=255)
                                                                                                                                gcggaattacacgacCGTTGTAGATATTGACTACTATTCCGCGTCGCGCCTCACGTGCAATATGAAGGTCGGGCAGAATACCGCCAGCAATATAGGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAGTTCAGCCGTCATGTTTa                 >  2:497088‑M2/16‑150 (MQ=255)
                                                                                                                                  ggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCCCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATc               >  2:1232879‑M2/14‑150 (MQ=255)
                                                                                                                                      gtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCtt           <  3:779491‑M2/141‑1 (MQ=255)
                                                                                                                                      gtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCtt           <  2:872275‑M2/141‑1 (MQ=255)
                                                                                                                                       tacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCttt          <  1:798849‑M2/142‑1 (MQ=255)
                                                                                                                                       tacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCttt          >  1:117340‑M2/9‑150 (MQ=255)
                                                                                                                                           cgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTtctc      <  3:503644‑M2/146‑1 (MQ=255)
                                                                                                                                           cgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTtctc      <  1:584724‑M2/146‑1 (MQ=255)
                                                                                                                                            gacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCa                                                                              <  3:799710‑M2/74‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGC  >  NC_000913_3_KO_pgi/3397523‑3397815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.